Team:GeorgiaTech/Biobricks

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     <td bgcolor="#964141" width="800"><font color="#FFFFFF"><p>The construct above is a fusion of the HybB cold shock promoter, the Ompa signal peptide, and AOXa.</p>
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     <td bgcolor="#964141" width="800"><font color="#FFFFFF"><p>The construct above is a fusion of the HybB cold shock promoter, the Ompa signal peptide, and AOXa. Aoxa is an alternative oxidase found in Sacred Lotus and shuffles electrons through an alternative path, thereby producing a thermogenesis event in Sacred Lotus. Our construct aims to produce an increase of heat above ambient temperature when cells expressing the construct are cold shocked (20c). The HybB promoter will express at lower temperatures (20 to 30c) but not at higher temperatures (37c).
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<p>More information can be found at the construct page: </p>
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<a href="http://partsregistry.org/Part:BBa_K410000" title="Bba_K140000"> Part:BBa_K410000 </a>
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     <td bgcolor="#964141" width="800"><font color="#FFFFFF"><p>The construct above contains with mRFP (red fluorescent protein) at the c-terminus of AOX will allow for imaging in the bacterial periplasm.</p>
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     <td bgcolor="#964141" width="800"><font color="#FFFFFF"><p>The construct above containing mRFP (red fluorescent protein) at the c-terminus of AOX will allow for imaging in the bacterial periplasm.</p>
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     <td bgcolor="#964141" width="800"><font color="#FFFFFF"><p>To quantify the activity of the HyBb promoter at different temperatures, we will express mRFP using HyBb in construct above. Note that the final BioBricks will be in the pSB1C3 assembly plasmid.</p>
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     <td bgcolor="#964141" width="800"><font color="#FFFFFF"><p>To quantify the activity of the HyBb promoter at different temperatures, we will express mRFP using HyBb in the construct above. Note that the final BioBricks will be in the pSB1C3 assembly plasmid.</p>
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<center><img src="https://static.igem.org/mediawiki/2010/7/77/ControlIGEM_7_2010.png" alt="construct" width="500" height="" img style="border: 2px solid black" img style="border: 2px solid white"></center>
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    <td bgcolor="#964141" width="800"><font color="#FFFFFF"><p>CONTROL: Picture shows nonlocalized mRFP expression throughout E Coli.  They carry the biobrick sequence for mRFP expression (which is why they are red.) in psb1a3. They were grown overnight at 37C.</p>
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<center><img src="https://static.igem.org/mediawiki/2010/5/51/ExpIGEM_8_2010.png" alt="construct" width="500" height="" img style="border: 2px solid black" img style="border: 2px solid white"></center>
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    <td bgcolor="#964141" width="800"><font color="#FFFFFF"><p>EXPERIMENTAL: Picture shows localized periplasmic mRFP expression in E coli.  These cells carry the coldshock promoter hybB, the targeting sequence for periplasmic expression ompA, and the red flourescent protein mRFP. This shows that we can direct the sequence to the periplasm. </p></center>
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<hr>
<hr>
<img src="https://static.igem.org/mediawiki/2010/a/a2/Sponserslogo.jpg" alt="sponsors" width="970" border="0" usemap="#Map3" />
<img src="https://static.igem.org/mediawiki/2010/a/a2/Sponserslogo.jpg" alt="sponsors" width="970" border="0" usemap="#Map3" />

Latest revision as of 02:48, 28 October 2010

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