"
Team:Freiburg Software/User/Robots/REBase
From 2010.igem.org
| Line 2: | Line 2: | ||
<html> | <html> | ||
| + | <!-- page special style --> | ||
| + | |||
| + | <style type="text/css"> | ||
| + | |||
| + | #subUserRobot a { | ||
| + | opacity: 0.92; | ||
| + | color: white; | ||
| + | } | ||
| + | |||
| + | </style> | ||
| + | |||
| + | <!-- Content starts here --> | ||
<div id=main_area> | <div id=main_area> | ||
| + | |||
<h1>REBase Robot</h1> | <h1>REBase Robot</h1> | ||
<div align="center" class="box_robot"> | <div align="center" class="box_robot"> | ||
| Line 14: | Line 27: | ||
Using REBase robot, user can find where in his sequence restriction sites attach to the sequence and also where the cut the sequence. REBase robot can be found at resenzymes-beta@appspot.com<br> | Using REBase robot, user can find where in his sequence restriction sites attach to the sequence and also where the cut the sequence. REBase robot can be found at resenzymes-beta@appspot.com<br> | ||
Before you start finding the details about the restriction sites using this robot, make sure that you have a sequence selected in the workspace.</p> | Before you start finding the details about the restriction sites using this robot, make sure that you have a sequence selected in the workspace.</p> | ||
| + | |||
<h2>Enzyme Selection</h2> | <h2>Enzyme Selection</h2> | ||
<p class="main"> | <p class="main"> | ||
| Line 33: | Line 47: | ||
In future, we plan to extend the functionality to let user select more than one Restriction enzymes at once. | In future, we plan to extend the functionality to let user select more than one Restriction enzymes at once. | ||
</p> | </p> | ||
| + | |||
</div> | </div> | ||
</html> | </html> | ||
Revision as of 11:03, 26 October 2010
REBase Robot
This page describes the use of the REBase Robot. If you would like to know how it works internally please click here.
Using REBase robot, user can find where in his sequence restriction sites attach to the sequence and also where the cut the sequence. REBase robot can be found at resenzymes-beta@appspot.com
Before you start finding the details about the restriction sites using this robot, make sure that you have a sequence selected in the workspace.
Enzyme Selection
This robot first identifies if the Restriction Enzymes Database is updated. If so it updates the local restriction enzymes database. Then it provides a list of enzymes to select from.
Restriction Enzymes identification
REBase robot searches for the entry pertaining the enzyme selected by the user in the local database. It then identifies the sequence and cut details of the enzyme. Then it goes through the sequence and finds all occurrences of the selected enzyme in the sequence. The output of the REBase robot is similar to the the following image. It displays the original sequence, followed by the sequence highlighted at Restriction Enzymes positions.
Note: The display may slightly differ from this picture due to ongoing development.
In future, we plan to extend the functionality to let user select more than one Restriction enzymes at once.
