Team:Freiburg Bioware/Project/Results

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<p><a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Results/Arming">Arming</a>The specifically targeted tumor cells were killed by prodrug activation approaches. Viral particles were charged with thymidine kinase and cytosine deaminase constructs to induce apoptosis in cancer cells upon delivery of ganciclovir or 5-Fluorocytosine, respectively.
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<p>The specifically targeted tumor cells were killed by prodrug activation approaches. Viral particles were charged with thymidine kinase and cytosine deaminase constructs to induce apoptosis in cancer cells upon delivery of ganciclovir or 5-Fluorocytosine, respectively.
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Revision as of 01:11, 28 October 2010

Results

Highlights

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Modularization

Gene of interest
The Virus Construction Kit enables researchers to encapsidate virtually any given DNA sequence into AAV-2 particles. As one example from therapeutic focus of our project, prodrug-activating enzymes are provided within the kit for efficient tumor cell killing. Additionally included fluorescent proteins allow monitoring of transduced cells by fluorescence microscopy and flow cytometry. Transgene expression can be fine-tuned using promoters of different specificity and enhancer elements also provided.
RepVP123
AAV-2 genes essential for the production of viral particles in a specialized cell line were identified by literature search, isolated on genetic level and modified to meet the requirements of BioBrick assembly. For this purpose, a specialized variant of the iGEM default backbone was created, proven functional and submitted.

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Targeting

One aim of our research is on the one hand to knock down the natural tropism of the Adeno-associates virus particles and on the other hand to specifically target tumor cells. This is achieved by genetic engineering of the virus surface. For this purpose, two different strategies were developed, including Targeting via Loops or fusion to the N-terminus of the viral protein VP2.

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Arming

The specifically targeted tumor cells were killed by prodrug activation approaches. Viral particles were charged with thymidine kinase and cytosine deaminase constructs to induce apoptosis in cancer cells upon delivery of ganciclovir or 5-Fluorocytosine, respectively.

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Tumor Killing

Lorem ipsum dolor sit amet, consectetuer adipiscing elit. Aenean commodo ligula eget dolor. Aenean massa. Cum sociis natoque penatibus et magnis dis parturient montes, nascetur ridiculus mus. Donec quam felis, ultricies nec, pellentesque eu, pretium quis, sem. Nulla consequat massa quis enim. Donec pede justo, fringilla vel, aliquet nec, vulputate eget, arcu. In enim justo, rhoncus ut, imperdiet a, venenatis vitae, justo. Nullam dictum felis eu pede mollis pretium. Integer tincidunt. Cras dapibus. Vivamus elementum semper nisi. Aenean vulputate eleifend tellus. Aenean leo ligula, porttitor eu, consequat vitae, eleifend ac, enim. Aliquam lorem ante, dapibus in, viverra quis, feugiat a, tellus. Phasellus viverra nulla ut metus varius laoreet. Quisque rutrum. Aenean imperdiet. Etiam ultricies nisi vel augue. Curabitur ullamcorper ultricies nisi. Nam eget dui. Etiam rhoncus. Maecenas tempus, tellus eget condimentum rhoncus, sem quam semper libero, sit amet adipiscing sem neque sed ipsum. Nam quam nunc, blandit vel, luctus pulvinar, hendrerit id, lorem. Maecenas nec odio et ante tincidunt tempus. Donec vitae sapien ut libero venenatis faucibus. Nullam quis ante. Etiam sit amet orci eget eros faucibus tincidunt. Duis leo. Sed fringilla mauris sit amet nibh. Donec sodales sagittis magna. Sed consequat, leo eget bibendum sodales, augue velit cursus nunc.

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