Team:Freiburg Bioware/NoteBook/Labjournal/March

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<li><a href="http://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/March">March (labday 1)</a></li>
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<li><a href="http://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/April">April (labday 2 - 5)</a></li>
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<center><a href="http://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal" ><img src="http://2010.igem.org/wiki/images/6/66/Freiburg10_Labjournal-Nav.png"  width="855px" style="border-bottom:1px solid #fff" /></a>
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<li><a href="http://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/May">May (labday 6 - 17)</a></li>
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<a href="http://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/March" ><img src="http://2010.igem.org/wiki/images/2/21/Freiburg10_Labjournal-March.png"  width="210px" margin: 5px 5px 5px 5px/></a>
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<li><a href="http://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/June">June (labday 18 - 45)</a></li>
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<a href="http://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/April" ><img src="http://2010.igem.org/wiki/images/6/6b/Freiburg10_Labjournal-April.png"  width="210px" margin: 5px 5px 5px 5px/></a>
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<li><a href="http://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/July">July (labday 46 - 75)</a></li>
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<a href="http://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/May" ><img src="http://2010.igem.org/wiki/images/6/61/Freiburg10_Labjournal-May.png"  width="210px" margin: 5px 5px 5px 5px/></a>
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<li><a href="http://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/August">August part 1 (labday 76 - 92)</a></li>
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<a href="http://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/June" ><img src="http://2010.igem.org/wiki/images/4/48/Freiburg10_Labjournal-June.png"  width="210px" margin: 5px 5px 5px 5px/></a>
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<li><a href="http://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/August2">August part 2 (labday 93 - 106)</a></li>
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<a href="http://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/July" ><img src="http://2010.igem.org/wiki/images/d/dc/Freiburg10_Labjournal-July.png"  width="210px" margin: 5px 5px 5px 5px/></a>
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<li><a href="http://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/September">September part 1 (labday 107 - 123)</a></li>
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<a href="http://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/August" ><img src="http://2010.igem.org/wiki/images/0/06/Freiburg10_Labjournal-AugustI.png"  width="210px" margin: 5px 5px 5px 5px/></a>
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<li><a href="http://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/September2">September part 2 (labday 124 - 135)</a></li>
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<a href="http://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/August2" ><img src="http://2010.igem.org/wiki/images/b/b1/Freiburg10_Labjournal-AugustII.png"  width="210px" margin: 5px 5px 5px 5px/></a>
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<li><a href="http://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/October">October part 1 (labday 136 - 149 )</a></li>
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<a href="http://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/September" ><img src="http://2010.igem.org/wiki/images/1/1e/Freiburg10_Labjournal-SeptemberI.png"  width="210px" margin: 5px 5px 5px 5px/></a>
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<li><a href="http://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/October2">October part 2 (labday 150 - 166 )</a></li>
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<a href="http://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/September2" ><img src="http://2010.igem.org/wiki/images/2/2e/Freiburg10_Labjournal-SeptemberII.png"  width="210px" margin: 5px 5px 5px 5px/></a>
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<li><a href="http://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/November">November  (labday 167 - 170 )</a></li>
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<a href="http://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/October" ><img src="http://2010.igem.org/wiki/images/6/65/Freiburg10_Labjournal-OctoberI.png"  width="210px" margin: 5px 5px 5px 5px/></a>
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<li><a href="http://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/Cellculture">Cellculture</a></li>
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<a href="http://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/October2" ><img src="http://2010.igem.org/wiki/images/4/48/Freiburg10_Labjournal-June.png"  width="210px" margin: 5px 5px 5px 5px/></a>
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===<p style="font-size:17px; background-color:#00dd77;">1. Labday 31.03.2010</p>===
===<p style="font-size:17px; background-color:#00dd77;">1. Labday 31.03.2010</p>===

Revision as of 21:04, 27 October 2010

1. Labday 31.03.2010

Sequence analysis

Investigators: Christian W., Bea, Kira, Achim, Kerstin, Hanna, Christian L. (Tobias, Kristian)


Theoretical cloning

Sequence analysis (Alignement) of Stratagene Vector with

  • AAV2
  • AAV5
  • AAV7
  • AAV8

Result:

Media:AAV_alignment.pdf
Notably, the alignment showed differences in the various AAV2 sequences, some of which are leading to frameshifts. The sequences date from 1983 and might not be completely accurate. We therefore rely on the Stratagene sequence from now on.

To do:

  • download and compare further AAV serotypes. (in Geneious)
  • compare literature
  • find out possible ways to modify the capsid
=> Go to Labjournal April (labday 2 - 5)