Team:Freiburg Bioware/NoteBook/Labjournal/March

From 2010.igem.org

(Difference between revisions)
(Sequence analysis)
(Sequence analysis)
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'''To do:'''
'''To do:'''
-
*download and compare further AAV serotypes. (In Geneious)
+
*download and compare further AAV serotypes. (in Geneious)
*compare literature
*compare literature
-
*find out possible ways to modify the Capsid
+
*find out possible ways to modify the capsid
<center>[https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/April '''=> Go to Labjournal April (labday 2 - 5)''']</center>
<center>[https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/April '''=> Go to Labjournal April (labday 2 - 5)''']</center>

Revision as of 17:51, 21 October 2010

=> Back to Notebook overview

1. Labday 31.03.2010

Sequence analysis

Investigators: Christian W., Bea, Kira, Achim, Kerstin, Hanna, Christian L. (Tobias, Kristian)


Theoretical cloning


Sequence analysis (Alignement) of Stratagene Vector with

  • AAV2
  • AAV5
  • AAV7
  • AAV8

result: Media:AAV_alignment.pdf
Notably, the alignment showed differences in the various AAV2 sequences, some of which are leading to frameshifts. The sequences date from 1983 and might not be completely accurate. We therefore rely on the Stratagene sequence from now on.

To do:

  • download and compare further AAV serotypes. (in Geneious)
  • compare literature
  • find out possible ways to modify the capsid
=> Go to Labjournal April (labday 2 - 5)