Team:Freiburg Bioware/NoteBook/Labjournal/March

From 2010.igem.org

(Difference between revisions)
 
(One intermediate revision not shown)
Line 5: Line 5:
<!-- Freiburg_bioware -->
<!-- Freiburg_bioware -->
 +
[http://2010.igem.org/Team:Freiburg_Bioware/NoteBook => Back to Notebook overview]<br><br>
<html>
<html>
 +
<div class="box_right">
 +
<left><u1>NoteBook Navigator</u1></left>
 +
<br>
 +
<ul>
<ul>
<li><a href="http://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/March">March (labday 1)</a></li>
<li><a href="http://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/March">March (labday 1)</a></li>
Line 49: Line 54:
*find out possible ways to modify the capsid
*find out possible ways to modify the capsid
-
<center>[http://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/April '''=> Go to Labjournal April (labday 2 - 5)''']</center>
+
<center>[http://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/April '''=> Go to Labjournal April (labday 2 - 5)''']</center><br><br><br><br>
<!-- End of page-->
<!-- End of page-->

Latest revision as of 22:18, 27 October 2010

=> Back to Notebook overview

NoteBook Navigator

1. Labday 31.03.2010

Sequence analysis

Investigators: Christian W., Bea, Kira, Achim, Kerstin, Hanna, Christian L. (Tobias, Kristian)


Theoretical cloning

Sequence analysis (Alignement) of Stratagene Vector with

  • AAV2
  • AAV5
  • AAV7
  • AAV8

Result:

Media:AAV_alignment.pdf
Notably, the alignment showed differences in the various AAV2 sequences, some of which are leading to frameshifts. The sequences date from 1983 and might not be completely accurate. We therefore rely on the Stratagene sequence from now on.

To do:

  • download and compare further AAV serotypes. (in Geneious)
  • compare literature
  • find out possible ways to modify the capsid
=> Go to Labjournal April (labday 2 - 5)