Team:Freiburg Bioware/NoteBook/Labjournal/March

From 2010.igem.org

(Difference between revisions)
(1. Labday 31.03.2010)
(Sequence analysis)
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[[Media:AAV_alignment.pdf]]
[[Media:AAV_alignment.pdf]]
<br>
<br>
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Curiously there were some significant differences in the aligned sequences of several AAV genomes  
+
The alignment showed significant differences in the sequences of several AAV genomes,
resulting in frame shifts.
resulting in frame shifts.
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Maybe because the sequences are from 1983 and therefore incomplete.
+
We decided to rely on the Stratagene sequence
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In case of doubt we count on the Stratagene-sequence.
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'''To do:'''
'''To do:'''
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*download and compare of further AAV serotypes. (In Geneious)
+
*download and compare further AAV serotypes. (In Geneious)
*compare literature
*compare literature
*find out which possibilities are given to modify the Capsid
*find out which possibilities are given to modify the Capsid

Revision as of 14:37, 14 September 2010

1. Labday 31.03.2010

Sequence analysis

Investigators: Christian W., Bea, Kira, Achim, Kerstin, Hanna, Christian L. (Tobias, Kristian)


Theoretical cloning


Sequence analysis (Alignement) of the Stratagene Vector with

  • AAV2
  • AAV5
  • AAV7
  • AAV8

result: Media:AAV_alignment.pdf
The alignment showed significant differences in the sequences of several AAV genomes, resulting in frame shifts. We decided to rely on the Stratagene sequence

To do:

  • download and compare further AAV serotypes. (In Geneious)
  • compare literature
  • find out which possibilities are given to modify the Capsid