Team:Freiburg Bioware/NoteBook/Labjournal/April

From 2010.igem.org

(Difference between revisions)
(New page: <!-- Freiburg_bioware --> <html> <!-- Stylesheed --> <style> #content { width: 965px; - →background-color: #3300ee;: } #banner_top { opacity: 1; width: 965px; height: 200px; margin: 0px ...)
Line 121: Line 121:
</div>
</div>
<div class="box_right">
<div class="box_right">
 +
<ul>
 +
<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/March">March</a></li>
 +
<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/April">April</a></li>
 +
<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/May">May</a></li>
 +
<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/June">June</a></li>
 +
<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/July">July</a></li>
 +
<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/August">August</a></li>
 +
<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/September">September</a></li>
 +
<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/October">October</a></li>
 +
<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/November">November</a></li>
 +
</ul>
 +
</html>
</html>
===2. Labday 13.04.2010===
===2. Labday 13.04.2010===

Revision as of 12:07, 6 September 2010

Contents

2. Labday 13.04.2010

The Biobricks Foundation: RFC

Investigators: Chris W., Bea, Kira, Hanna, Julian, Anna, Jessica (Tobias,Sven, Kristian)



The BioBricks Foundation is dedicated to promoting and protecting the open development,
sharing, and reuse of BioBrick™ standard biological parts.
Homepage: [[1]]

Two important standarts:

BBF RFC-10:
This standard defines the required sequence properties for a Biobrick(tm) standard biological part.
[[2]]

BBF RFC-25: Fusion Protein (Freiburg) Biobrick assembly standard
This Request for Comments (RFC) describes an extension to the original BioBrick assembly standard (BBF RFC 10).
Media:Freiburg10 BBF RFC 25.pdf


Theoretical cloning
DNA- and protein-analyse:

  • Thymidin Kinase HSVI

Accesion: V00470 (Genebank) CDS without iGEM-restriction site, unkown sequence in front of CDS with an EcoRI-restriction site, sequence from 1980! (there are a lot of X-ray analysis, but just to be on the safe side we should check the sequence)

  • Cytosine Deaminase (see also FCY1) (S.cerevisae)

Accession number: AF005261 (Genebank) CDS without iGEM-restriction site, unkown in front of CDS with PstI-restriction site, sequence from 1997

  • pORF-CodA::upp (E.coli) [Vektor from InvivoGen]

CDS of the Cytosine Deaminase (E.coli) without iGEM-restriction site

  • Alignment of the amino acid-sequences of the cytosin deaminases of E.coli (Invivogen) and S.cerevisiae (Genebank)

no consensus found at the amino acid-sequence discussion at the next meeting

3. Labday 15.04.2010

Theoretical cloning

Investigators: Kira, Johannes, Bea



4. Labday 22.04.2010

Theoretical cloning

Investigators: Adrian, Chris W., Hanna, Bea



  • tomorrow we will obtain the vector sequence for the thymidinkinase (WT) from Amor
  • call at Stratagene: #240071 AAV-Helper Free System, 1412 € (netto), available until next week ; we asked for a discount (Bea got Infos per E-mail)
  • next step (tomorrow, 10 am): modification of the multiple cloning site


5. Labday 23.04.2010

Theoretical cloning

Investiators: Adrian, Chris W., Hanna, Kerstin, Anissa, (Kristian, Tobias, Sven)


we won't use this kinase...

Retrieved from "http://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/April"