http://2010.igem.org/wiki/index.php?title=Team:Freiburg_Bioware/BioBricks/vector_plasmid&feed=atom&action=historyTeam:Freiburg Bioware/BioBricks/vector plasmid - Revision history2024-03-29T11:12:41ZRevision history for this page on the wikiMediaWiki 1.16.5http://2010.igem.org/wiki/index.php?title=Team:Freiburg_Bioware/BioBricks/vector_plasmid&diff=203794&oldid=prevNiklas at 01:16, 28 October 20102010-10-28T01:16:56Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><h1>Assembled Vectorplasmid - ready for transfection</h1></center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><h1>Assembled Vectorplasmid - ready for transfection</h1></center></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="width:935px; height:300px; border: 1px solid black;"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="width:935px; height:300px; border: 1px solid black;"></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For the development of retargeted viral vectors the vectorplasmid often functions as generator for different read-outs that can be measured in several assays. For this purpose it is often desired to use fully assembled vectorplasmids that are confirmed working and provieded in our Virus Construction Kit.<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For the development of retargeted viral vectors the vectorplasmid often functions as generator for different read-outs that can be measured in several assays. For this purpose it is often desired to use fully assembled vectorplasmids that are confirmed working and provieded in our Virus Construction Kit.<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>There for we assembled vectorplasmids with either fluorescent proteins like mCerulean(CFP), mVenus(YFP) and mCherry or produrg convertases like the cytosine deaminase or the tymindine kinase. <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>There for we assembled vectorplasmids with either fluorescent proteins like mCerulean(CFP), mVenus(YFP) and mCherry or produrg convertases like the cytosine deaminase or the tymindine kinase. <br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Detection of productive infections is possible by measuring the fluorescence of the fluorescent proteins or the number of killed cells where as the detection of the vectorplasmid is in any step of the infection possible by performing a qPCR reaction with primers for the CMV promoter.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Detection of productive infections is possible by measuring the fluorescence of the fluorescent proteins or the number of killed cells where as the detection of the vectorplasmid is in any step of the infection possible by performing a qPCR reaction with primers for the CMV promoter.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div style="float:right; width:400px; height:auto; /*border: 1px solid black;*/"><img src="http://partsregistry.org/wiki/images/2/2c/Freiburg10_Ue_Vector_plasmid.png" width="400" /></div></div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div style="float:right; width:400px; height:auto<ins class="diffchange diffchange-inline">; margin-top: 80px</ins>;/*border: 1px solid black;*/"><img src="http://partsregistry.org/wiki/images/2/2c/Freiburg10_Ue_Vector_plasmid.png" width="400" /></div></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K404119" target="_blank"><img src="http://partsregistry.org/wiki/images/0/03/Freiburg10_Vectorplasmid_composite_1.png" height="117px"/></a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K404119" target="_blank"><img src="http://partsregistry.org/wiki/images/0/03/Freiburg10_Vectorplasmid_composite_1.png" height="117px"/></a></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K404120" target="_blank"><img src="http://partsregistry.org/wiki/images/a/ad/Freiburg10_Vectorplasmid_composite_2.png" height="117px"/></a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K404120" target="_blank"><img src="http://partsregistry.org/wiki/images/a/ad/Freiburg10_Vectorplasmid_composite_2.png" height="117px"/></a></div></td></tr>
</table>Niklashttp://2010.igem.org/wiki/index.php?title=Team:Freiburg_Bioware/BioBricks/vector_plasmid&diff=203712&oldid=prevNiklas at 01:14, 28 October 20102010-10-28T01:14:37Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><h1>Create your own Vectorplasmid with the PrecursorBricks</h1></center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><h1>Create your own Vectorplasmid with the PrecursorBricks</h1></center></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For several applications users of the Virus Construction Kit will only want to insert their own GOI and not change the promoter and regulatory elements in a first experiment. For these applications we provide precursors of our vectorplasmid that reduce the required cloning on two steps. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For several applications users of the Virus Construction Kit will only want to insert their own GOI and not change the promoter and regulatory elements in a first experiment. For these applications we provide precursors of our vectorplasmid that reduce the required cloning on two steps. </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div style="float:right; width:400px; /*border: 1px solid black;*/"><img src="http://partsregistry.org/wiki/images/1/1c/Freiburg10_Vectorplasmid_cloning.png" width="400" /></div></div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div style="float:right; width:400px<ins class="diffchange diffchange-inline">; margin-top: 10px</ins>;/*border: 1px solid black;*/"><img src="http://partsregistry.org/wiki/images/1/1c/Freiburg10_Vectorplasmid_cloning.png" width="400" /></div></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K404114" target="_blank"><img src="http://partsregistry.org/wiki/images/2/2f/Freiburg10_Vectorplasmid_precursors_1.png" height="90px"/></a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K404114" target="_blank"><img src="http://partsregistry.org/wiki/images/2/2f/Freiburg10_Vectorplasmid_precursors_1.png" height="90px"/></a></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K404115" target="_blank"><img src="http://partsregistry.org/wiki/images/3/38/Freiburg10_Vectorplasmid_precursors_2.png" height="90px"/></a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K404115" target="_blank"><img src="http://partsregistry.org/wiki/images/3/38/Freiburg10_Vectorplasmid_precursors_2.png" height="90px"/></a></div></td></tr>
</table>Niklashttp://2010.igem.org/wiki/index.php?title=Team:Freiburg_Bioware/BioBricks/vector_plasmid&diff=203580&oldid=prevNiklas at 01:10, 28 October 20102010-10-28T01:10:42Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><h1>Vectorplasmid BioBricks</h1></center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><h1>Vectorplasmid BioBricks</h1></center></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="width:935px; height:310px; border: 1px solid black;"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="width:935px; height:310px; border: 1px solid black;"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div style="float:left; width:500px; margin: <del class="diffchange diffchange-inline">0px </del>10px 0px 10px; text-align:justify;"> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div style="float:left; width:500px; margin: <ins class="diffchange diffchange-inline">50px </ins>10px 0px 10px; text-align:justify;"> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Vectorplasmid BioBricks allow it to reconstitute a vectorplasmid of your choice. Either the provided parts are assembled or an other Gene of Interest (GOI) can be integrated into an vectorplasmid. Beside the packaging capacity of 4.7 kbb, the possiblilities are virtually endless. <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Vectorplasmid BioBricks allow it to reconstitute a vectorplasmid of your choice. Either the provided parts are assembled or an other Gene of Interest (GOI) can be integrated into an vectorplasmid. Beside the packaging capacity of 4.7 kbb, the possiblilities are virtually endless. <br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The Inverted Terminal Repeats (ITRs) are constructs form highly stable secondary structures that are recognized by the Rep proteins and function as a packiging signal. There for the ITRs have to flank the later vectorplasmid. <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The Inverted Terminal Repeats (ITRs) are constructs form highly stable secondary structures that are recognized by the Rep proteins and function as a packiging signal. There for the ITRs have to flank the later vectorplasmid. <br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The Beta-globin and the human growth hormon polyadenylation sequence (hGH)are regulatory sequences that are ment to enhace the expression of the used GOI.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The Beta-globin and the human growth hormon polyadenylation sequence (hGH)are regulatory sequences that are ment to enhace the expression of the used GOI.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div style="float:right; width:400px; /*border: 1px solid black;*/"><img src="http://partsregistry.org/wiki/images/4/47/Freiburg10_Vectorplasmidassembly.png" width="400" </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div style="float:right; width:400px<ins class="diffchange diffchange-inline">; margin-top: 10px</ins>;/*border: 1px solid black;*/"><img src="http://partsregistry.org/wiki/images/4/47/Freiburg10_Vectorplasmidassembly.png" width="400" </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>height="auto"/></div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>height="auto"/></div></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a href="http://partsregistry.org/Part:BBa_K404100" target="_blank"><img src="http://partsregistry.org/wiki/images/b/ba/Freiburg10_VectorplasmidBrick_1.png" height="90px"/></a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a href="http://partsregistry.org/Part:BBa_K404100" target="_blank"><img src="http://partsregistry.org/wiki/images/b/ba/Freiburg10_VectorplasmidBrick_1.png" height="90px"/></a></div></td></tr>
</table>Niklashttp://2010.igem.org/wiki/index.php?title=Team:Freiburg_Bioware/BioBricks/vector_plasmid&diff=184486&oldid=prevTimon at 14:39, 27 October 20102010-10-27T14:39:24Z<p></p>
<a href="http://2010.igem.org/wiki/index.php?title=Team:Freiburg_Bioware/BioBricks/vector_plasmid&diff=184486&oldid=132807">Show changes</a>Timonhttp://2010.igem.org/wiki/index.php?title=Team:Freiburg_Bioware/BioBricks/vector_plasmid&diff=132807&oldid=prevVolkerMorath at 14:46, 24 October 20102010-10-24T14:46:49Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br><br><br><!---VectorplasmidBricks---></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br><br><br><!---VectorplasmidBricks---></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><h1>Vectorplasmid BioBricks</h1></center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><h1>Vectorplasmid BioBricks</h1></center></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div style="width:935px; height:<del class="diffchange diffchange-inline">320px</del>; border: 1px solid black;"></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div style="width:935px; height:<ins class="diffchange diffchange-inline">310px</ins>; border: 1px solid black;"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="float:left; width:500px; height:auto; margin: 0px 10px 0px 10px; text-align:justify;"> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="float:left; width:500px; height:auto; margin: 0px 10px 0px 10px; text-align:justify;"> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Vectorplasmid BioBricks allow it to reconstitute a vectorplasmid of your choice. Either the provided parts are assembled or an other Gene of Interest (GOI) can be integrated into an vectorplasmid. Beside the packaging capacity of 4.7 kbb, the possiblilities are virtually endless. <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Vectorplasmid BioBricks allow it to reconstitute a vectorplasmid of your choice. Either the provided parts are assembled or an other Gene of Interest (GOI) can be integrated into an vectorplasmid. Beside the packaging capacity of 4.7 kbb, the possiblilities are virtually endless. <br></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br><br><br><!---Vectorplasmid precursors---></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br><br><br><!---Vectorplasmid precursors---></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><h1>Create your own Vectorplasmid with the PrecursorBricks</h1></center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><h1>Create your own Vectorplasmid with the PrecursorBricks</h1></center></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div style="width:935px; height:<del class="diffchange diffchange-inline">320px</del>;border: 1px solid black; "></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div style="width:935px; height:<ins class="diffchange diffchange-inline">300px</ins>;border: 1px solid black; "></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="float:left; width:500px; height:auto; margin: 0px 10px 0px 10px; text-align:justify;"> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="float:left; width:500px; height:auto; margin: 0px 10px 0px 10px; text-align:justify;"> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For several applications users of the Virus Construction Kit will only want to insert their own GOI and not change the promoter and regulatory elements in a first experiment. For these applications we provide precursors of our vectorplasmid that reduce the required cloning on two steps. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For several applications users of the Virus Construction Kit will only want to insert their own GOI and not change the promoter and regulatory elements in a first experiment. For these applications we provide precursors of our vectorplasmid that reduce the required cloning on two steps. </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br><br><br><!---Vectorplasmids assembled---></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br><br><br><!---Vectorplasmids assembled---></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><h1>Assembled Vectorplasmid - ready for transfection</h1></center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><h1>Assembled Vectorplasmid - ready for transfection</h1></center></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div style="width:935px; height:<del class="diffchange diffchange-inline">320px</del>; border: 1px solid black;"></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div style="width:935px; height:<ins class="diffchange diffchange-inline">300px</ins>; border: 1px solid black;"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="float:left; width:500px; height:auto; margin: 0px 10px 0px 10px; text-align:justify;"> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="float:left; width:500px; height:auto; margin: 0px 10px 0px 10px; text-align:justify;"> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For the development of retargeted viral vectors the vectorplasmid often functions as generator for different read-outs that can be measured in several assays. For this purpose it is often desired to use fully assembled vectorplasmids that are confirmed working and provieded in our Virus Construction Kit.<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For the development of retargeted viral vectors the vectorplasmid often functions as generator for different read-outs that can be measured in several assays. For this purpose it is often desired to use fully assembled vectorplasmids that are confirmed working and provieded in our Virus Construction Kit.<br></div></td></tr>
</table>VolkerMorathhttp://2010.igem.org/wiki/index.php?title=Team:Freiburg_Bioware/BioBricks/vector_plasmid&diff=131746&oldid=prevVolkerMorath at 12:52, 24 October 20102010-10-24T12:52:17Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="float:left; width:500px; height:auto; margin: 0px 10px 0px 10px; text-align:justify;"> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="float:left; width:500px; height:auto; margin: 0px 10px 0px 10px; text-align:justify;"> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For the development of retargeted viral vectors the vectorplasmid often functions as generator for different read-outs that can be measured in several assays. For this purpose it is often desired to use fully assembled vectorplasmids that are confirmed working and provieded in our Virus Construction Kit.<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For the development of retargeted viral vectors the vectorplasmid often functions as generator for different read-outs that can be measured in several assays. For this purpose it is often desired to use fully assembled vectorplasmids that are confirmed working and provieded in our Virus Construction Kit.<br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>There for we assembled vectorplasmids with fluorescent proteins like <del class="diffchange diffchange-inline"> </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>There for we assembled vectorplasmids with <ins class="diffchange diffchange-inline">either </ins>fluorescent proteins like <ins class="diffchange diffchange-inline"> mCerulean(CFP), mVenus(YFP) and mCherry or produrg convertases like the cytosine deaminase or the tymindine kinase. <br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Detection of productive infections is possible by measuring the fluorescence of the fluorescent proteins or the number of killed cells where as the detection of the vectorplasmid is in any step of the infection possible by performing a qPCR reaction with primers for the CMV promoter.</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="float:right; width:400px; height:auto; /*border: 1px solid black;*/"><img src="http://partsregistry.org/wiki/images/2/2c/Freiburg10_Ue_Vector_plasmid.png" width="400" </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="float:right; width:400px; height:auto; /*border: 1px solid black;*/"><img src="http://partsregistry.org/wiki/images/2/2c/Freiburg10_Ue_Vector_plasmid.png" width="400" </div></td></tr>
</table>VolkerMorathhttp://2010.igem.org/wiki/index.php?title=Team:Freiburg_Bioware/BioBricks/vector_plasmid&diff=131711&oldid=prevVolkerMorath at 12:46, 24 October 20102010-10-24T12:46:18Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="width:935px; height:320px; border: 1px solid black;"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="width:935px; height:320px; border: 1px solid black;"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="float:left; width:500px; height:auto; margin: 0px 10px 0px 10px; text-align:justify;"> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="float:left; width:500px; height:auto; margin: 0px 10px 0px 10px; text-align:justify;"> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"><!</del>-<del class="diffchange diffchange-inline">--Insert Text </del>in <del class="diffchange diffchange-inline">here!!! ---</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">For the development of retargeted viral vectors the vectorplasmid often functions as generator for different read</ins>-<ins class="diffchange diffchange-inline">outs that can be measured </ins>in <ins class="diffchange diffchange-inline">several assays. For this purpose it is often desired to use fully assembled vectorplasmids that are confirmed working and provieded in our Virus Construction Kit.<br</ins>></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">There for we assembled vectorplasmids with fluorescent proteins like </ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="float:right; width:400px; height:auto; /*border: 1px solid black;*/"><img src="http://partsregistry.org/wiki/images/2/2c/Freiburg10_Ue_Vector_plasmid.png" width="400" </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="float:right; width:400px; height:auto; /*border: 1px solid black;*/"><img src="http://partsregistry.org/wiki/images/2/2c/Freiburg10_Ue_Vector_plasmid.png" width="400" </div></td></tr>
</table>VolkerMorathhttp://2010.igem.org/wiki/index.php?title=Team:Freiburg_Bioware/BioBricks/vector_plasmid&diff=131659&oldid=prevVolkerMorath at 12:39, 24 October 20102010-10-24T12:39:29Z<p></p>
<table style="background-color: white; color:black;">
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br><br><br><!---VectorplasmidBricks---></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br><br><br><!---VectorplasmidBricks---></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><h1>Vectorplasmid BioBricks</h1></center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><h1>Vectorplasmid BioBricks</h1></center></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div style="width:935px; height:320px; "></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div style="width:935px; height:320px<ins class="diffchange diffchange-inline">; border: 1px solid black</ins>;"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="float:left; width:500px; height:auto; margin: 0px 10px 0px 10px; text-align:justify;"> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="float:left; width:500px; height:auto; margin: 0px 10px 0px 10px; text-align:justify;"> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Vectorplasmid BioBricks allow it to reconstitute a vectorplasmid of your choice. Either the provided parts are assembled or an other Gene of Interest (GOI) can be integrated into an vectorplasmid. Beside the packaging capacity of 4.7 kbb, the possiblilities are virtually endless. <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Vectorplasmid BioBricks allow it to reconstitute a vectorplasmid of your choice. Either the provided parts are assembled or an other Gene of Interest (GOI) can be integrated into an vectorplasmid. Beside the packaging capacity of 4.7 kbb, the possiblilities are virtually endless. <br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The Inverted Terminal Repeats (ITRs) are constructs form highly stable secondary structures that are recognized by the Rep proteins and function as a packiging signal. There for the ITRs have to flank the later vectorplasmid. <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The Inverted Terminal Repeats (ITRs) are constructs form highly stable secondary structures that are recognized by the Rep proteins and function as a packiging signal. There for the ITRs have to flank the later vectorplasmid. <br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The Beta-globin and the human growth hormon polyadenylation sequence (hGH)are regulatory sequences that are ment to enhace the expression of the used GOI.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The Beta-globin and the human growth hormon polyadenylation sequence (hGH)are regulatory sequences that are ment to enhace the expression of the used GOI.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="float:right; width:400px; height:auto; /*border: 1px solid black;*/"><img src="http://partsregistry.org/wiki/images/4/47/Freiburg10_Vectorplasmidassembly.png" width="400" </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="float:right; width:400px; height:auto; /*border: 1px solid black;*/"><img src="http://partsregistry.org/wiki/images/4/47/Freiburg10_Vectorplasmidassembly.png" width="400" </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br><br><br><!---Vectorplasmid precursors---></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br><br><br><!---Vectorplasmid precursors---></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><h1>Create your own Vectorplasmid with the PrecursorBricks</h1></center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><h1>Create your own Vectorplasmid with the PrecursorBricks</h1></center></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div style="width:935px; height:320px; "></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div style="width:935px; height:320px<ins class="diffchange diffchange-inline">;border: 1px solid black</ins>; "></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="float:left; width:500px; height:auto; margin: 0px 10px 0px 10px; text-align:justify;"> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="float:left; width:500px; height:auto; margin: 0px 10px 0px 10px; text-align:justify;"> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For several applications users of the Virus Construction Kit will only want to insert their own GOI and not change the promoter and regulatory elements in a first experiment. For these applications we provide precursors of our vectorplasmid that reduce the required cloning on two steps. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For several applications users of the Virus Construction Kit will only want to insert their own GOI and not change the promoter and regulatory elements in a first experiment. For these applications we provide precursors of our vectorplasmid that reduce the required cloning on two steps. </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br><br><br><!---Vectorplasmids assembled---></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br><br><br><!---Vectorplasmids assembled---></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><h1>Assembled Vectorplasmid - ready for transfection</h1></center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><h1>Assembled Vectorplasmid - ready for transfection</h1></center></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div style="width:935px; height:320px; "></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div style="width:935px; height:320px<ins class="diffchange diffchange-inline">; border: 1px solid black</ins>;"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="float:left; width:500px; height:auto; margin: 0px 10px 0px 10px; text-align:justify;"> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="float:left; width:500px; height:auto; margin: 0px 10px 0px 10px; text-align:justify;"> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><!---Insert Text in here!!! ---></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><!---Insert Text in here!!! ---></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="float:right; width:400px; height:auto; /*border: 1px solid black;*/"><img src="http://partsregistry.org/wiki/images/2/2c/Freiburg10_Ue_Vector_plasmid.png" width="400" </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="float:right; width:400px; height:auto; /*border: 1px solid black;*/"><img src="http://partsregistry.org/wiki/images/2/2c/Freiburg10_Ue_Vector_plasmid.png" width="400" </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>height="auto"/></div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>height="auto"/></div></div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K404119" target="_blank"><img src="http://partsregistry.org/wiki/images/0/03/Freiburg10_Vectorplasmid_composite_1.png" width="auto" </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K404119" target="_blank"><img src="http://partsregistry.org/wiki/images/0/03/Freiburg10_Vectorplasmid_composite_1.png" width="auto" </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>height="117px"/></a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>height="117px"/></a></div></td></tr>
</table>VolkerMorathhttp://2010.igem.org/wiki/index.php?title=Team:Freiburg_Bioware/BioBricks/vector_plasmid&diff=131652&oldid=prevVolkerMorath at 12:38, 24 October 20102010-10-24T12:38:17Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For several applications users of the Virus Construction Kit will only want to insert their own GOI and not change the promoter and regulatory elements in a first experiment. For these applications we provide precursors of our vectorplasmid that reduce the required cloning on two steps. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For several applications users of the Virus Construction Kit will only want to insert their own GOI and not change the promoter and regulatory elements in a first experiment. For these applications we provide precursors of our vectorplasmid that reduce the required cloning on two steps. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div style="float:right; width:<del class="diffchange diffchange-inline">480px</del>; height:auto; /*border: 1px solid black;*/"><img src="http://partsregistry.org/wiki/images/1/1c/Freiburg10_Vectorplasmid_cloning.png" width="<del class="diffchange diffchange-inline">480</del>" </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div style="float:right; width:<ins class="diffchange diffchange-inline">400px</ins>; height:auto; /*border: 1px solid black;*/"><img src="http://partsregistry.org/wiki/images/1/1c/Freiburg10_Vectorplasmid_cloning.png" width="<ins class="diffchange diffchange-inline">400</ins>" </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>height="auto"/></div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>height="auto"/></div></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K404114" target="_blank"><img src="http://partsregistry.org/wiki/images/2/2f/Freiburg10_Vectorplasmid_precursors_1.png" width="auto" </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K404114" target="_blank"><img src="http://partsregistry.org/wiki/images/2/2f/Freiburg10_Vectorplasmid_precursors_1.png" width="auto" </div></td></tr>
</table>VolkerMorathhttp://2010.igem.org/wiki/index.php?title=Team:Freiburg_Bioware/BioBricks/vector_plasmid&diff=131647&oldid=prevVolkerMorath at 12:37, 24 October 20102010-10-24T12:37:57Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="width:935px; height:320px; "></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="width:935px; height:320px; "></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="float:left; width:500px; height:auto; margin: 0px 10px 0px 10px; text-align:justify;"> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="float:left; width:500px; height:auto; margin: 0px 10px 0px 10px; text-align:justify;"> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Vector plasmid </del>BioBricks allow it to reconstitute a <del class="diffchange diffchange-inline">vector plasmid </del>of your choice. Either the provided parts are assembled or an other Gene of Interest (GOI) can be integrated into an vectorplasmid. Beside the packaging capacity of 4.7 kbb, the possiblilities are virtually endless. <br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Vectorplasmid </ins>BioBricks allow it to reconstitute a <ins class="diffchange diffchange-inline">vectorplasmid </ins>of your choice. Either the provided parts are assembled or an other Gene of Interest (GOI) can be integrated into an vectorplasmid. Beside the packaging capacity of 4.7 kbb, the possiblilities are virtually endless. <br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The Inverted Terminal Repeats (ITRs) are constructs form highly stable secondary structures that are recognized by the Rep proteins and function as a packiging signal. There for the ITRs have to flank the later <del class="diffchange diffchange-inline">vector plasmid</del>. <br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The Inverted Terminal Repeats (ITRs) are constructs form highly stable secondary structures that are recognized by the Rep proteins and function as a packiging signal. There for the ITRs have to flank the later <ins class="diffchange diffchange-inline">vectorplasmid</ins>. <br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The Beta-globin and the human growth hormon polyadenylation sequence (hGH)are regulatory sequences that are ment to enhace the expression of the used GOI.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The Beta-globin and the human growth hormon polyadenylation sequence (hGH)are regulatory sequences that are ment to enhace the expression of the used GOI.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 49:</td>
<td colspan="2" class="diff-lineno">Line 49:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="width:935px; height:320px; "></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="width:935px; height:320px; "></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="float:left; width:500px; height:auto; margin: 0px 10px 0px 10px; text-align:justify;"> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="float:left; width:500px; height:auto; margin: 0px 10px 0px 10px; text-align:justify;"> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"><!---Insert Text </del>in <del class="diffchange diffchange-inline">here!!! ---></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">For several applications users of the Virus Construction Kit will only want to insert their own GOI and not change the promoter and regulatory elements </ins>in <ins class="diffchange diffchange-inline">a first experiment. For these applications we provide precursors of our vectorplasmid that reduce the required cloning on two steps. </ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="float:right; width:480px; height:auto; /*border: 1px solid black;*/"><img src="http://partsregistry.org/wiki/images/1/1c/Freiburg10_Vectorplasmid_cloning.png" width="480" </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="float:right; width:480px; height:auto; /*border: 1px solid black;*/"><img src="http://partsregistry.org/wiki/images/1/1c/Freiburg10_Vectorplasmid_cloning.png" width="480" </div></td></tr>
</table>VolkerMorath