"
Team:Freiburg Bioware/BioBricks/vector plasmid
From 2010.igem.org
(Difference between revisions)
VolkerMorath (Talk | contribs) |
VolkerMorath (Talk | contribs) |
||
| Line 14: | Line 14: | ||
<div style="float:left; width:500px; height:auto; margin: 0px 10px 0px 10px; text-align:justify;"> | <div style="float:left; width:500px; height:auto; margin: 0px 10px 0px 10px; text-align:justify;"> | ||
Vector plasmid BioBricks allow it to reconstitute a vector plasmid of your choice. Either the provided parts are assembled or an other Gene of Interest (GOI) can be integrated into an vectorplasmid. Beside the packaging capacity of 4.7 kbb, the possiblilities are virtually endless. <br> | Vector plasmid BioBricks allow it to reconstitute a vector plasmid of your choice. Either the provided parts are assembled or an other Gene of Interest (GOI) can be integrated into an vectorplasmid. Beside the packaging capacity of 4.7 kbb, the possiblilities are virtually endless. <br> | ||
| - | The Inverted Terminal Repeats (ITRs) are constructs | + | The Inverted Terminal Repeats (ITRs) are constructs form highly stable secondary structures that are recognized by the Rep proteins and function as a packiging signal. There for the ITRs have to flank the later vector plasmid. <br> |
| + | The Beta-globin and the human growth hormon polyadenylation sequence (hGH)are regulatory sequences that are ment to enhace the expression of the used GOI. | ||
</div> | </div> | ||
Revision as of 12:30, 24 October 2010
Vectorplasmid BioBricks
Vector plasmid BioBricks allow it to reconstitute a vector plasmid of your choice. Either the provided parts are assembled or an other Gene of Interest (GOI) can be integrated into an vectorplasmid. Beside the packaging capacity of 4.7 kbb, the possiblilities are virtually endless.
The Inverted Terminal Repeats (ITRs) are constructs form highly stable secondary structures that are recognized by the Rep proteins and function as a packiging signal. There for the ITRs have to flank the later vector plasmid.
The Beta-globin and the human growth hormon polyadenylation sequence (hGH)are regulatory sequences that are ment to enhace the expression of the used GOI.
The Inverted Terminal Repeats (ITRs) are constructs form highly stable secondary structures that are recognized by the Rep proteins and function as a packiging signal. There for the ITRs have to flank the later vector plasmid.
The Beta-globin and the human growth hormon polyadenylation sequence (hGH)are regulatory sequences that are ment to enhace the expression of the used GOI.
Create your own Vectorplasmid with the PrecursorBricks
Assembled Vectorplasmid - ready for transfection
