Team:Freiburg Bioware


Revision as of 18:16, 26 October 2010 by Timon (Talk | contribs)

Gene delivery using viral vectors holds great promise for the treatment of acquired and inherited diseases. The human Adeno-Associated Virus (AAV) is a small, non-pathogenic, single-stranded DNA virus gaining increasing attention being both versatile and effective. Taking current knowledge into account, we generated a recombinant, modularized, BioBrick-compatible AAV ‘Virus Construction Kit’. We provide parts for modified capsid proteins, targeting modules, tumor-specific promoters, and prodrug-activating enzymes as well as readily assembled vectors for gene delivery and production of non-replicative virus particles. The viral tropism is altered by N-terminal fusion or by loop replacement of the capsid proteins. Functionality of viruses constructed from our kit was demonstrated by fluorescent protein expression in infected cells and by prodrug-induced killing of tumor cells upon viral delivery of a thymidine kinase. Incorporating multiple layers of safety, we provide a general tool to the growing field of personalized medicine and demonstrate its use in tumor therapy.

Project Results

This Site is in Construction, later you will find more right here...[more]

This Site is in Construction, later on you will find more right here...[Klick for more]

This Site is in Construction, later you will find more right here...[Klick for more]

Virus Construction Kit - The Manual

This site is under construction, later you will find more right here...[more]

Statistics - In a Minute

  • What happened in the lab during iGEM?

  • Longest Wikipage: Longest Wikipage with 222,795 bytes
  • Number of Biobricks: 116 Number of BioBricks
  • Number of Ordered Oligos: 193 Number of ordered Oligo-Nucleotides
  • Mini-Preps total: 1085 Minipreps total
  • Midi-Preps total: 106 Midipreps total
  • Highest Midi-Prep Concentration:
    5045,6 ng/µL Highest Prep concentration (Midi)
  • Number of Glycerolstocks: 763 Number of Glycerolstocks
  • Number of Team members: 15 Number of Team members
  • Days in the lab: 170 Days in the lab
  • Longest Labday: 18h Longest Labday
  • Coffee Consumed During iGEM : 2238 Cups Coffee consumed during iGEM


Modularization and modification of the viral capsids for retargeting approaches and directed gene delivery of suicide genes resulted in many different BioBricks.
Click here to explore them.


The aspect of biological safety was considered well and a risk assessment and security profile for the Adeno-associated Virus were created. To hear about this click here.


Only cloning and cell culture?

No! Besides BioBrick assembly, flow cytometry analysis, quantification based methos via ELISA or Western Blot and cytotoxicity assays have been performed
(go to Methods)


Fun and Science at the same time?

Yes! The iGEM team Freiburg did not only share the lab bench, but as well some nice days canoeing and initiated the photo contest Cuckoo Clock Competition.


Uni Potsdam Uni Freiburg Bioss kuk.lab daad frias Roche Qiagen Starlab peqlab Eppendorf Zeiss Gilson Fermentas aigilent Avidity Hiss Biozym GATC Geneart ATG purimex dkfz Mathworks

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