Team:Freiburg Bioware

From 2010.igem.org

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'''Short project description''': <br>
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In the last years the number of people affected by cancer increased significantly. About 25% cases of death are caused by malign tumors. Even though the industry is putting great efforts into developing effective strategies against cancer, innovative methods to fight tumors are needed. <br>
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<div class="virus"><img src="https://static.igem.org/mediawiki/2010/2/24/Freiburg10_rot250trans.gif"  title="virus (3.5MB)" id="animated_virus"/></div>
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This year’s aim of '''Freiburg Bioware team 2010''' is to create a universal virus construction kit which can be designed by submitted BioBricks. Our goal is to knockout the natural tropism of adeno-associated virus particles and to specifically target tumor cells by genetic engineering of the viral surface. The recombinant viruses will contain different genes coding for enzymes which convert harmless drugs into toxic molecules in targeted cell and kill the tumor cells. This approach will be a great advance in improving the growing field of personalized medicine.  
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Gene delivery using viral vectors holds great promise for the treatment of acquired and inherited diseases. The human Adeno-Associated Virus (AAV) is a small, non-pathogenic, single-stranded DNA virus gaining increasing attention being both versatile and effective. Taking current knowledge into account, we generated a recombinant, modularized, BioBrick-compatible AAV ‘Virus Construction Kit’. We provide parts for modified capsid proteins, targeting modules, tumor-specific promoters, and prodrug-activating enzymes as well as readily assembled vectors for gene delivery and production of non-replicative virus particles. The viral tropism is altered by N-terminal fusion or by loop replacement of the capsid proteins. Functionality of viruses constructed from our kit was demonstrated by fluorescent protein expression in infected cells and by prodrug-induced killing of tumor cells upon viral delivery of a thymidine kinase. Incorporating multiple layers of safety, we provide a general tool to the growing field of personalized medicine and demonstrate its use in tumor therapy.
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<!---Box on the upper left: Project Results--->
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<div class="box_home">
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<a href="https://2010.igem.org/Team:Freiburg_Bioware/Project"><img class="right" src="https://static.igem.org/mediawiki/2010/5/5f/Freiburg10_Virus_Logo_Small.png" id="ccc" />
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</a>
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<a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Results"><h2>Project Results</h2></a>
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<p><!--<a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Results#highlights"><h5>Highlights: </h5></a>-->
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Team Freiburg presents a functional, modular Virus Construction Kit for specifically targeting and killing tumor cells. <a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Results#highlights"><span class="bold">[more]</span></a></p>
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<p><!--<a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Results#highlights"><span class="bold"><h5>Heading:</h5></span></a>-->
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Structural engineering of the three dimensional viral capsid by introducing four distinct targeting sites led to successful differential transduction of tumor cells. <a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Results#targeting"><span class="bold">[more]</span></a></p>
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<p><!--<a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Virus_Construction_Kit"><span class="bold"><h5>Head-Line:</h5></span></a>-->
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Efficient gene-directed enzyme prodrug activation was demonstrated by BioBrick-compatible constructs introduced into tumor cells. <a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Results#arming"><span class="bold">[more]</span></a></p>
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</div>
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<!---Big box on the left: Virus Construction Kit - Manual--->
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<div class="box_long">
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<a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Virus_Construction_Kit">
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<img class="right" src="https://static.igem.org/mediawiki/2010/f/fb/Freiburg10_Manual_Logo_small.png" id="ccc" /></a>
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<a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Virus_Construction_Kit"><h2>Virus Construction Kit - The Manual</h2></a>
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<p><!--<a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Virus_Construction_Kit"><h5>Heading:</h5></a>-->
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Build your own therapeutical virus – 150 pages of knowledge. <a href="https://2010.igem.org/Team:Freiburg_Bioware/Project/Virus_Construction_Kit"><span class="bold">[more]</span></a></p>
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<!--- News Ticker --->
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<div class="box_long box_long_news news_ticker">
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<a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Statistics" style="z-index:20;position:relative;"><h2>Statistics - In a Minute</h2></a>
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<ul id="news" class="newsticker">
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  <li><div class="news"><img class="right" src="https://static.igem.org/mediawiki/2010/0/0b/Freiburg10_Number_of_Plasmids.png" id="ccc" /><p>
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What happened in the lab during iGEM? </p>
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</div></li>
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<li><div class="news"><img
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src="https://static.igem.org/mediawiki/2010/a/a5/Freiburg10_Longest_Wikipage.png"
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alt="Longest Wikipage" width="368"><p>Longest Wikipage:<br />with 222,795 bytes</p></div>
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</li>
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<li><div class="news"><img src="https://static.igem.org/mediawiki/2010/1/10/Freiburg10_Number_of_Biobricks.png"
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alt="Number of BioBricks" width="368"><p>Number of Biobricks:<br /> 116</p></div></li>
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<li><div class="news"><img heigth="100px" src="https://static.igem.org/mediawiki/2010/3/3f/Freiburg10_Number_of_ordered_Oligo-Nucleotides.png"
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alt="Number of ordered Oligo-Nucleotides" width="184"><p>Number of Ordered Oligos:<br /> 193</p></div></li>
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<li><div class="news"><img
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src="https://static.igem.org/mediawiki/2010/f/f3/Freiburg10_Minipreps_total.png"
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alt="Minipreps total" width="184"><p>Mini-Preps total:<br /> 1085</p></div></li>
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<li><div class="news"><img
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src="https://static.igem.org/mediawiki/2010/f/f7/Freiburg10_Midipreps_total.png"
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alt="Midipreps total" width="368"><p>Midi-Preps total:<br /> 106</p></div></li>
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<li><div class="news"><img
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src="https://static.igem.org/mediawiki/2010/d/d6/Freiburg10_Highest_Prep_concentration.png"
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alt="Highest Prep concentration (Midi)" width="368"><p>Highest Midi-Prep Concentration:<br />5045,6 ng/µL </p></div></li>
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<li><div class="news"><img
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src="https://static.igem.org/mediawiki/2010/a/af/Freiburg10_Number_of_Glyzerolstocks.png"
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alt="Number of Glycerolstocks" width="368"><p>Number of Glycerolstocks:<br /> 763 </p></div></li>
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<li><div class="news"><img
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src="https://static.igem.org/mediawiki/2010/6/60/Freiburg10_Number_of_Team_members.png"
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alt="Number of Team members" width="368"><p>Number of Team members:<br /> 15 </p></div></li>
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<li><div class="news"><img
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src="https://static.igem.org/mediawiki/2010/0/03/Freiburg10_Days_in_the_lab.png"
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alt="Days in the lab" width="368"><p>Days in the lab:<br /> 170 </p></div></li>
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<li><div class="news"><img
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src="https://static.igem.org/mediawiki/2010/d/d1/Freiburg10_Longest_Labday.png"
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alt="Longest Labday" width="368"><p>Longest Labday:<br />18h </p></div></</li>
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<li><div class="news"><img height="100px"
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src="https://static.igem.org/mediawiki/2010/b/b5/Freiburg10_Coffee_consumed_during_iGEM.png"
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alt="Coffee consumed during iGEM" width="184"><p>Coffee Consumed During iGEM:<br /> 2238 Cups</p></div></li>
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<li><div class="news"><img heigth="100px"class="right" src="https://static.igem.org/mediawiki/2010/5/5f/Freiburg10_Virus_Logo_Small.png" id="ccc" /><p><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Statistics"><h5>!Freiburg Bioware Statistics!</h5></a></p></div></li>
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</ul>
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</div>
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<div class="clearfix"></div>
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<html>
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<div height="290px">
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<a href="http://www2.clustrmaps.com/counter/maps.php?url=https://2010.igem.org/Team:Freiburg_Bioware"
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<!---Box on the upper right: BioBricks--->
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  id="clustrMapsLink">
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<div class="box_home_small">
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  <img src="http://www2.clustrmaps.com/counter/index2.php?url=https://2010.igem.org/Team:Freiburg_Bioware"  
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<a href="https://2010.igem.org/Team:Freiburg_Bioware/BioBricks"><img class="right" src="https://static.igem.org/mediawiki/2010/d/d1/Freiburg_10_BioBrick_icon_small.png" id="ccc" /></a>
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<a href="https://2010.igem.org/Team:Freiburg_Bioware/BioBricks"><h2>BioBricks</h2></a>
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  alt="Locations of visitors to this page" title="Locations of visitors to this page" id="clustrMapsImg"
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<p class="standard">Modularization and modification of the viral capsids for retargeting approaches and directed gene delivery of suicide genes resulted in many different BioBricks.<br> Click here to explore them. <a href="https://2010.igem.org/Team:Freiburg_Bioware/BioBricks"><span class="bold">[more]</a></span></p>
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  onerror="this.onerror=null; this.src='http://clustrmaps.com/images/clustrmaps-back-soon.jpg'; document.getElementById('clustrMapsLink').href='http://clustrmaps.com';" />
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</a>
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<!---Box on the lower left: Biosafety--->
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<div class="box_home_small">
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<a href="https://2010.igem.org/Team:Freiburg_Bioware/Safety"><img class="right" src="https://static.igem.org/mediawiki/2010/f/f9/Freiburg10_Danger_Virus_small.png" id="ccc" /></a>
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<a href="https://2010.igem.org/Team:Freiburg_Bioware/Safety"><h2>Biosafety</h2></a>
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<p class="standard">Aspects of biological safety were considered throughout and a risk assessment and security profile of the Adeno-associated Virus were created. Read more about Biosafety issues. <a href="https://2010.igem.org/Team:Freiburg_Bioware/Safety"><span class="bold">[more]</span></a></p>
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</div>
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<!---Box on the lower right: Notebook--->
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<div class="box_home_small">
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<a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook">
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<img class="right" src="https://static.igem.org/mediawiki/2010/9/95/Freiburg_10_Notebook_image_small.png" id="ccc" /></a>
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<a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook">
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<h2>Notebook</h2></a>
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<p>
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<a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook"><span class="bold">From DNA to virus particle to cell culture? </span></a><br />
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Yes! Details on cloning, cell culture, flow cytometry analyses, quantifications by ELISA or Western Blot, cytotoxicity assays, time resolved microscopy ... <a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Methods"><span class="bold">[more]</span></a></p>
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</div>
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{|align="justify"
 
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
 
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|[[Image:Freiburg_Bioware_logo.png|200px|right|frame]]
 
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''Tell us more about your project.  Give us background.  Use this as the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
 
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|[[Image:Freiburg_Bioware_team.png|right|frame|Your team picture]]
 
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|align="center"|[[Team:Freiburg_Bioware | Team Example]]
 
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<!--- The Mission, Experiments --->
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<!---Box on the lower right: Team--->
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<div class="box_home_small">
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<a href="https://2010.igem.org/Team:Freiburg_Bioware/Team/Team_2010">
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<img class="right" src="https://static.igem.org/mediawiki/2010/b/bd/Freiburg10_Virus_Logo_Small_small.png" id="ccc" /></a>
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<a href="https://2010.igem.org/Team:Freiburg_Bioware/Team/Team_2010">
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<h2>Team</h2></a>
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<p><a href="https://2010.igem.org/Team:Freiburg_Bioware/Team/Team_2010"><span class="bold">Fun and Science at the same time?</span></a></a><br />
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Yes! The iGEM team Freiburg did not only share the lab bench, but as well some nice days <a href="https://2010.igem.org/Team:Freiburg_Bioware/Team/Photo_Gallery"><span class="bold">canoeing</span></a> and initiated the photo contest Cuckoo Clock Competition. <a href="https://2010.igem.org/Team:Freiburg_Bioware/Team/Cuckoo_Clock"><span class="bold">[more]</span></a></p>
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</div>
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<div class="clearfix"></div>
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<br />
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<h1>Sponsors</h1>
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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<div class="map" height="1169px">
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!align="center"|[[Team:Freiburg_Bioware|Home]]
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<map name="Sponsoren_iGEM" id="Sponsoren_iGEM">
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!align="center"|[[Team:Freiburg_Bioware/Team|Team]]
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<area shape="circle" coords="705,369,65" href="http://www.uni-potsdam.de/" target="_blank" alt="Uni Potsdam" title="Uni Potsdam" />
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!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=Freiburg_Bioware Official Team Profile]
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<area shape="circle" coords="156,138,121" href="http://www.uni-freiburg.de/start-en.html?set_language=en" target="_blank" alt="Uni Freiburg" title="Uni Freiburg" />
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!align="center"|[[Team:Freiburg_Bioware/Project|Project]]
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<area shape="rect" coords="304,8,815,269" href="http://www.bioss.uni-freiburg.de/cms/index.php#" target="_blank" alt="Bioss" title="Bioss" />
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!align="center"|[[Team:Freiburg_Bioware/Parts|Parts Submitted to the Registry]]
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<area shape="rect" coords="501,311,611,434" href="http://www.syntbio.net/" target="_blank" alt="kuk.lab" title="kuk.lab" />
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!align="center"|[[Team:Freiburg_Bioware/Modeling|Modeling]]
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<area shape="rect" coords="274,329,480,412" href="http://www.daad.de/en/index.html" alt="daad" target="_blank" title="daad" />
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!align="center"|[[Team:Freiburg_Bioware/Notebook|Notebook]]
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<area shape="rect" coords="67,324,238,419" href="http://www.frias.uni-freiburg.de/" alt="frias" target="_blank" title="frias" />
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!align="center"|[[Team:Freiburg_Bioware/Safety|Safety]]
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<area shape="rect" coords="95,534,376,686" href="http://www.roche.com/index.htm" target="_blank" alt="Roche" title="Roche" />
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|}
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<area shape="rect" coords="510,458,677,606" href="http://www.qiagen.com/default_qs.aspx" target="_blank" alt="Qiagen" title="Qiagen" />
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<area shape="rect" coords="477,671,708,752" href="http://www.starlab.de/int/?l=2" target="_blank" alt="Starlab" title="Starlab" />
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<area shape="rect" coords="551,799,787,861" href="http://www.peqlab.com" target="_blank" alt="peqlab" title="peqlab" />
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<area shape="rect" coords="291,801,494,859" href="http://www.eppendorf.com/int/?l=1&amp;action=start" target="_blank" alt="Eppendorf" title="Eppendorf" />
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<area shape="rect" coords="43,801,226,859" href="http://www.gilson.com/en/" target="_blank" alt="Gilson" title="Gilson" />
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<area shape="rect" coords="44,872,218,936" href="http://www.fermentas.de/index.php?&amp;language=en" target="_blank" alt="Fermentas" title="Fermentas" />
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<area shape="rect" coords="42,942,259,1002" href="http://www.home.agilent.com" target="_blank" alt="aigilent" title="aigilent" />
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<area shape="rect" coords="289,958,388,1041" href="http://www.avidity.com/" target="_blank" alt="Avidity" title="Avidity" />
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<area shape="rect" coords="412,960,566,1048" href="http://www.hiss-dx.de/hiss/index.php?id=43&amp;L=1" target="_blank" alt="Hiss" title="Hiss" />
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<area shape="rect" coords="591,981,795,1043" href="http://www.biozym.com/site/21/Produkte.aspx" target="_blank" alt="Biozym" title="Biozym" />
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<area shape="rect" coords="592,876,750,956" href="http://www.gatc-biotech.com" target="_blank" alt="GATC" title="GATC" />
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<area shape="rect" coords="296,886,540,948" href="http://www.geneart.com/" target="_blank" alt="Geneart" title="Geneart" />
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<area shape="rect" coords="495,1070,808,1151" href="http://www.atg-biosynthetics.com/" target="_blank" alt="ATG" title="ATG" />
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<area shape="rect" coords="242,1086,469,1154" href="http://www.purimex.com/" alt="purimex" target="_blank" title="purimex" />
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<area shape="rect" coords="45,1084,219,1154" href="http://www.dkfz.de/index.html" alt="dkfz" target="_blank" title="dkfz" />
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<area shape="rect" coords="46,1021,264,1075" href="http://www.mathworks.com" target="_blank" alt="Mathworks" title="Mathworks" />
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</map>
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<img src="https://static.igem.org/mediawiki/2010/4/49/Freiburg10_Sponsoren_Image_Map.gif" width="826" height="1169" border="0" alt="" title="" usemap="#Sponsoren_iGEM" />
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</div>
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<center><a href="http://www2.clustrmaps.com/counter/maps.php?url=https://2010.igem.org/Team:Freiburg_Bioware" id="clustrMapsLink"><img src="http://www2.clustrmaps.com/counter/index2.php?url=https://2010.igem.org/Team:Freiburg_Bioware" style="border:0px;" alt="Locations of visitors to this page" title="Locations of visitors to this page" id="clustrMapsImg" onerror="this.onerror=null; this.src='http://clustrmaps.com/images/clustrmaps-back-soon.jpg'; document.getElementById('clustrMapsLink').href='http://clustrmaps.com';" />
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</a></center>
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<center><small>primary team contact: Kristian M. Müller; <a href="mailto:Kristian@syntbio.net">e-mail: Kristian@syntbio.net</a></small></center>
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</html>
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{{:Team:Freiburg_Bioware/Footer}}

Latest revision as of 15:23, 14 January 2011

Gene delivery using viral vectors holds great promise for the treatment of acquired and inherited diseases. The human Adeno-Associated Virus (AAV) is a small, non-pathogenic, single-stranded DNA virus gaining increasing attention being both versatile and effective. Taking current knowledge into account, we generated a recombinant, modularized, BioBrick-compatible AAV ‘Virus Construction Kit’. We provide parts for modified capsid proteins, targeting modules, tumor-specific promoters, and prodrug-activating enzymes as well as readily assembled vectors for gene delivery and production of non-replicative virus particles. The viral tropism is altered by N-terminal fusion or by loop replacement of the capsid proteins. Functionality of viruses constructed from our kit was demonstrated by fluorescent protein expression in infected cells and by prodrug-induced killing of tumor cells upon viral delivery of a thymidine kinase. Incorporating multiple layers of safety, we provide a general tool to the growing field of personalized medicine and demonstrate its use in tumor therapy.

Project Results

Team Freiburg presents a functional, modular Virus Construction Kit for specifically targeting and killing tumor cells. [more]

Structural engineering of the three dimensional viral capsid by introducing four distinct targeting sites led to successful differential transduction of tumor cells. [more]

Efficient gene-directed enzyme prodrug activation was demonstrated by BioBrick-compatible constructs introduced into tumor cells. [more]

Virus Construction Kit - The Manual

Build your own therapeutical virus – 150 pages of knowledge. [more]

Statistics - In a Minute

  • What happened in the lab during iGEM?

  • Longest Wikipage

    Longest Wikipage:
    with 222,795 bytes

  • Number of BioBricks

    Number of Biobricks:
    116

  • Number of ordered Oligo-Nucleotides

    Number of Ordered Oligos:
    193

  • Minipreps total

    Mini-Preps total:
    1085

  • Midipreps total

    Midi-Preps total:
    106

  • Highest Prep concentration (Midi)

    Highest Midi-Prep Concentration:
    5045,6 ng/µL

  • Number of Glycerolstocks

    Number of Glycerolstocks:
    763

  • Number of Team members

    Number of Team members:
    15

  • Days in the lab

    Days in the lab:
    170

  • Longest Labday

    Longest Labday:
    18h

  • Coffee consumed during iGEM

    Coffee Consumed During iGEM:
    2238 Cups

  • !Freiburg Bioware Statistics!

BioBricks

Modularization and modification of the viral capsids for retargeting approaches and directed gene delivery of suicide genes resulted in many different BioBricks.
Click here to explore them. [more]

Biosafety

Aspects of biological safety were considered throughout and a risk assessment and security profile of the Adeno-associated Virus were created. Read more about Biosafety issues. [more]

Notebook

From DNA to virus particle to cell culture?
Yes! Details on cloning, cell culture, flow cytometry analyses, quantifications by ELISA or Western Blot, cytotoxicity assays, time resolved microscopy ... [more]

Team

Fun and Science at the same time?
Yes! The iGEM team Freiburg did not only share the lab bench, but as well some nice days canoeing and initiated the photo contest Cuckoo Clock Competition. [more]


Sponsors

Uni Potsdam Uni Freiburg Bioss kuk.lab daad frias Roche Qiagen Starlab peqlab Eppendorf Gilson Fermentas aigilent Avidity Hiss Biozym GATC Geneart ATG purimex dkfz Mathworks


Locations of visitors to this page

primary team contact: Kristian M. Müller; e-mail: Kristian@syntbio.net

Retrieved from "http://2010.igem.org/Team:Freiburg_Bioware"