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Team:Edinburgh/Notebook/Red light sensor
From 2010.igem.org
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== Red Light Sensor == | == Red Light Sensor == | ||
| + | <br><br> | ||
| + | <b>04/10/10</b> | ||
<br> | <br> | ||
| + | Chris has managed to construct what we hope is a working red light sensor. It is being sent in for sequencing today. <br> | ||
| + | Minor characterisation experiment: | ||
| + | -Red light sensor constructed with blue-white lacZ reporter | ||
| + | -If activated, the red light sensor transformants should produce blue colonies | ||
| + | -Red light sensor containing-colonies from transformant plate streaked onto two plates | ||
| + | -One plate left in the light, the other left in the dark | ||
| + | -If the one in the light grows blue and the one in the dark doesn't, it is a good indication that the construct is working<br><br> | ||
Revision as of 11:57, 4 October 2010
Red Light Sensor
04/10/10
Chris has managed to construct what we hope is a working red light sensor. It is being sent in for sequencing today.
Minor characterisation experiment:
-Red light sensor constructed with blue-white lacZ reporter
-If activated, the red light sensor transformants should produce blue colonies
-Red light sensor containing-colonies from transformant plate streaked onto two plates
-One plate left in the light, the other left in the dark
-If the one in the light grows blue and the one in the dark doesn't, it is a good indication that the construct is working
