Team:Edinburgh/Notebook/Red light sensor

From 2010.igem.org

(Difference between revisions)
(Red Light Sensor)
(Red Light Sensor)
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Chris has managed to construct what we hope is a working red light sensor. It is being sent in for sequencing today. <br>
Chris has managed to construct what we hope is a working red light sensor. It is being sent in for sequencing today. <br>
-
Minor characterisation experiment:
+
Minor characterisation experiment:<br>
-
-Red light sensor constructed with blue-white lacZ reporter
+
-Red light sensor constructed with blue-white lacZ reporter<br>
-
-If activated, the red light sensor transformants should produce blue colonies
+
-If activated, the red light sensor transformants should produce blue colonies<br>
-
-Red light sensor containing-colonies from transformant plate streaked onto two plates
+
-Red light sensor containing-colonies from transformant plate streaked onto two plates<br>
-
-One plate left in the light, the other left in the dark
+
-One plate left in the light, the other left in the dark<br>
-If the one in the light grows blue and the one in the dark doesn't, it is a good indication that the construct is working<br><br>
-If the one in the light grows blue and the one in the dark doesn't, it is a good indication that the construct is working<br><br>

Revision as of 11:57, 4 October 2010





Red Light Sensor



04/10/10
Chris has managed to construct what we hope is a working red light sensor. It is being sent in for sequencing today.
Minor characterisation experiment:
-Red light sensor constructed with blue-white lacZ reporter
-If activated, the red light sensor transformants should produce blue colonies
-Red light sensor containing-colonies from transformant plate streaked onto two plates
-One plate left in the light, the other left in the dark
-If the one in the light grows blue and the one in the dark doesn't, it is a good indication that the construct is working