Team:Edinburgh/Notebook/Green light sensor

From 2010.igem.org

(Difference between revisions)
(New page: {{:Team:Edinburgh/defaultcss}} {{:Team:Edinburgh/defaultadvancedcss}} {{:Team:Edinburgh/dropdowncss}} <html> <head> <style> h2{ background-color: white; margin: 0 ! important; border-left...)
 
(6 intermediate revisions not shown)
Line 43: Line 43:
   <li><a href="https://2010.igem.org/Team:Edinburgh/BioBricks#Genomic">submitted parts</a></li>
   <li><a href="https://2010.igem.org/Team:Edinburgh/BioBricks#Genomic">submitted parts</a></li>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Results#Genomic">results</a></li>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Results#Genomic">results</a></li>
-
   <li><a href="https://2010.igem.org/Team:Edinburgh/Project/Future">future work</a></li>
+
   <li><a href="https://2010.igem.org/Team:Edinburgh/Project/Future">the future</a></li>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Project/References">references</a></li>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Project/References">references</a></li>
   </ul>
   </ul>
Line 50: Line 50:
  <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial" class="dir">bacterial BRIDGEs</a>
  <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial" class="dir">bacterial BRIDGEs</a>
   <ul>
   <ul>
-
   <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Core_repressilator">the repressilator</a></li>
+
   <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Core_repressilator">the project</a></li>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Red_light_producer">red light</a></li>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Red_light_producer">red light</a></li>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Red_light_sensor">red sensor</a></li>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Red_light_sensor">red sensor</a></li>
Line 59: Line 59:
   <li><a href="https://2010.igem.org/Team:Edinburgh/BioBricks#Bacterial">submitted parts</a></li>
   <li><a href="https://2010.igem.org/Team:Edinburgh/BioBricks#Bacterial">submitted parts</a></li>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Results#Bacterial">results</a></li>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Results#Bacterial">results</a></li>
-
   <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Future">future work</a></li>
+
   <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Future">the future</a></li>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/References">references</a></li>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/References">references</a></li>
   </ul>
   </ul>
Line 70: Line 70:
   <li><a href="https://2010.igem.org/Team:Edinburgh/Modelling/Bacterial">the bacterial model</a></li>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Modelling/Bacterial">the bacterial model</a></li>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Modelling/Signalling">the signalling model</a></li>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Modelling/Signalling">the signalling model</a></li>
 +
  <li><a href="https://2010.igem.org/Team:Edinburgh/Modelling/Tools">tools</a></li>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Results#Modelling">results</a></li>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Results#Modelling">results</a></li>
-
   <li><a href="https://2010.igem.org/Team:Edinburgh/Modelling/Future">future work</a></li>
+
   <li><a href="https://2010.igem.org/Team:Edinburgh/Modelling/Future">the future</a></li>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Modelling/References">references</a></li>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Modelling/References">references</a></li>
   </ul>
   </ul>
Line 78: Line 79:
  <li><a href="https://2010.igem.org/Team:Edinburgh/Human" class="dir">human BRIDGEs</a>
  <li><a href="https://2010.igem.org/Team:Edinburgh/Human" class="dir">human BRIDGEs</a>
   <ul>
   <ul>
-
  <li><a href="https://2010.igem.org/Team:Edinburgh/Human/Aspects">human aspects</a></li>
 
   <li><a href="https://2010.igem.org/Team:Edinburgh/Human/Communication">communication of science</a></li>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Human/Communication">communication of science</a></li>
-
   <li><a href="https://2010.igem.org/Team:Edinburgh/Human/Terminology">terminology research</a></li>
+
   <li><a href="https://2010.igem.org/Team:Edinburgh/Human/Branding">iGEM survey</a></li>
-
  <li><a href="https://2010.igem.org/Team:Edinburgh/Human/Wiki">wiki</a></li>
+
   <li><a href="https://2010.igem.org/Team:Edinburgh/Human/Conversations">conversations</a></li>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Human/Conversations">conversations</a></li>
-
  <li><a href="https://2010.igem.org/Team:Edinburgh/Human/Identity">identity</a></li>
 
-
  <li><a href="https://2010.igem.org/Team:Edinburgh/Human/Collaboration">collaboration</a></li>
 
-
  <li><a href="https://2010.igem.org/Team:Edinburgh/Human/Branding">branding research</a></li>
 
-
  <li><a href="https://2010.igem.org/Team:Edinburgh/Human/SciFi">science fiction writing</a></li>
 
-
  <li><a href="https://2010.igem.org/Team:Edinburgh/Human/FutureApps">future applications</a></li>
 
-
  <li><a href="https://2010.igem.org/Team:Edinburgh/Human/SelfReflection">self-reflection</a></li>
 
   <li><a href="https://2010.igem.org/Team:Edinburgh/Human/Epic">the epic</a></li>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Human/Epic">the epic</a></li>
-
   <li><a href="https://2010.igem.org/Team:Edinburgh/Results#Human">results</a></li>
+
   <li><a href="https://2010.igem.org/Team:Edinburgh/Human/FutureApps">future applications</a></li>
-
   <li><a href="https://2010.igem.org/Team:Edinburgh/Human/Future">future work</a></li>
+
   <li><a href="https://2010.igem.org/Team:Edinburgh/Results#Human">further thoughts</a></li>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Human/References">references</a></li>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Human/References">references</a></li>
   </ul>
   </ul>
Line 122: Line 115:
<br>
<br>
<br>
<br>
 +
 +
<div id="windowbox" style="border: .2em solid #660000; padding: 5px; position:fixed; top:50%; right:30px; width:8%;">
 +
<span style="color:ivory;">These <b>notes</b> correspond to the part of the <b>project</b> detailed <a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Green_light_sensor">here</a>.</span>
 +
 +
</div>
</html>
</html>
==Green Light Sensor==
==Green Light Sensor==
 +
<br>
 +
 +
 +
'''8/10/2010'''
 +
* running purified PCR: marker (lane 1), PhOR (Lane 2), Ccas (Lane 3).
 +
 +
[[Image:148.JPG]]
 +
 +
'''19/10/20010'''
 +
* Lane 1: marker
 +
* Lane 2: PheA (very faint band or primer smear?)
 +
* Lane 3: Ccas + PhoR ligation
 +
 +
[[Image:173.JPG]]
 +
 +
'''22/10/2010'''
 +
* PhoA + LacZ
 +
 +
[[Image:174.JPG]]
 +
 +
'''23/10/2010'''
 +
* Lane 1: marker
 +
* Lane 2: phA promoter + lac Z
 +
* rest- irrelevant
 +
 +
[[Image:175.JPG]]
 +
 +
'''25/10/2010'''
 +
* pCB + green light sensor
 +
 +
[[Image:176.JPG]]
 +
 +
'''26/10/2010'''
 +
* total green light sensor + reporter
 +
 +
[[Image:177.JPG]]
 +
 +
'''27/10/2010'''
 +
* green light sensor ligations into PSB1C3
 +
 +
[[Image:178.JPG]]
 +
<br>
<br>

Latest revision as of 02:12, 28 October 2010





These notes correspond to the part of the project detailed here.

Green Light Sensor



8/10/2010

  • running purified PCR: marker (lane 1), PhOR (Lane 2), Ccas (Lane 3).

148.JPG

19/10/20010

  • Lane 1: marker
  • Lane 2: PheA (very faint band or primer smear?)
  • Lane 3: Ccas + PhoR ligation

173.JPG

22/10/2010

  • PhoA + LacZ

174.JPG

23/10/2010

  • Lane 1: marker
  • Lane 2: phA promoter + lac Z
  • rest- irrelevant

175.JPG

25/10/2010

  • pCB + green light sensor

176.JPG

26/10/2010

  • total green light sensor + reporter

177.JPG

27/10/2010

  • green light sensor ligations into PSB1C3

178.JPG