Team:Edinburgh/Notebook/Blue light producer

From 2010.igem.org

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(Blue Light Producer)
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   <ul>
   <ul>
   <li><a href="http://2010.igem.org/Team:Edinburgh/Human/Aspects">human aspects</a></li>
   <li><a href="http://2010.igem.org/Team:Edinburgh/Human/Aspects">human aspects</a></li>
 +
  <li><a href="http://2010.igem.org/Team:Edinburgh/Human/Communication">communication of science</a></li>
 +
  <li><a href="http://2010.igem.org/Team:Edinburgh/Human/Terminology">terminology research</a></li>
 +
  <li><a href="http://2010.igem.org/Team:Edinburgh/Human/Wiki">wiki</a></li>
 +
  <li><a href="http://2010.igem.org/Team:Edinburgh/Human/Conversations">conversations</a></li>
 +
  <li><a href="http://2010.igem.org/Team:Edinburgh/Human/Identity">identity</a></li>
 +
  <li><a href="http://2010.igem.org/Team:Edinburgh/Human/Collaboration">collaboration</a></li>
 +
  <li><a href="http://2010.igem.org/Team:Edinburgh/Human/Branding">branding research</a></li>
 +
  <li><a href="http://2010.igem.org/Team:Edinburgh/Human/SciFi">science fiction writing</a></li>
 +
  <li><a href="http://2010.igem.org/Team:Edinburgh/Human/FutureApps">future applications</a></li>
 +
  <li><a href="http://2010.igem.org/Team:Edinburgh/Human/SelfReflection">self-reflection</a></li>
   <li><a href="http://2010.igem.org/Team:Edinburgh/Human/Epic">the epic</a></li>
   <li><a href="http://2010.igem.org/Team:Edinburgh/Human/Epic">the epic</a></li>
   <li><a href="http://2010.igem.org/Team:Edinburgh/Results#Human">results</a></li>
   <li><a href="http://2010.igem.org/Team:Edinburgh/Results#Human">results</a></li>

Revision as of 11:08, 12 October 2010





Blue Light Producer


13/7/2010

Empty edinbrick vector for control.

  • LuxAB + edinbrick ligation.

28/7/2010

an update on LuxAB:

  • The transformations of Edinbrick are fine but all the luxABs failed (grew blue, not white on Xgal and did not glow, as of 27/7)/
  • Still have some digested LuxAB-ediI so re-did ligation and transformation.

Gel Pic- minipreps

Lane 1&15 Lane 2 Others
Ladder EdiI LuxAB

GEL PIC

Lane 1 Lane 2 +3 Lanes 3-6
Ladder EdiI LuxAB
  • The ligation had the same gel characteristics as previous luxAb-ediI ligations, e.x. it isn't visivle- not hopeful of this transformation working

29/7/2010

12/8/2010 update on LumP:

  • still getting pink/red colonies from transformants
  • single digests give a band of about 3kb
  • double digests give a band of about 2.5 kb -highly confusing
  • and also, no sign on RFP despite red/pink growth.
  • Vector wa ssequenced and LumP is definately there.

gel pohot no x and y + description.