Team:Edinburgh/BioBricks

From 2010.igem.org

(Difference between revisions)
Line 153: Line 153:
  <tr>
  <tr>
   <td align="left"><b>cat</b></td>
   <td align="left"><b>cat</b></td>
-
   <td>Protein Domain</td>
+
   <td>Translational Unit</td>
   <td><a href="http://partsregistry.org/Part:BBa_K322210">BBa_K322210</a></td>
   <td><a href="http://partsregistry.org/Part:BBa_K322210">BBa_K322210</a></td>
   <td>Chloramphenicol acetyltransferase (chloramphenicol resistance gene).</td>
   <td>Chloramphenicol acetyltransferase (chloramphenicol resistance gene).</td>
Line 206: Line 206:
  <tr>
  <tr>
   <td align="left"><b>luxAB</b></td>
   <td align="left"><b>luxAB</b></td>
-
   <td>Protein Domain</td>
+
   <td>Translational Unit</td>
   <td><a href="http://partsregistry.org/Part:BBa_K322139">BBa_K322139</a></td>
   <td><a href="http://partsregistry.org/Part:BBa_K322139">BBa_K322139</a></td>
-
   <td>Bacterial luciferase producing blue light.</td>
+
   <td>Bacterial luciferase producing blue light (from <i>X. luminescens</i>).</td>
  </tr>
  </tr>
  <tr>
  <tr>
Line 218: Line 218:
  <tr>
  <tr>
   <td align="left"><b>lumP</b></td>
   <td align="left"><b>lumP</b></td>
-
   <td>Protein Domain</td>
+
   <td>Translational Unit</td>
   <td><a href="http://partsregistry.org/Part:BBa_K322007">BBa_K322007</a></td>
   <td><a href="http://partsregistry.org/Part:BBa_K322007">BBa_K322007</a></td>
-
   <td>Lumazine protein to shift the wavelength of LuxAB.</td>
+
   <td>Lumazine protein to shift the wavelength of LuxAB to blue.</td>
  </tr>
  </tr>
  <tr>
  <tr>
-
   <td align="left"><b>Phycobilin synthesis operon</b></td>
+
   <td align="left"><b>Phycocyanobilin synthesis operon</b></td>
-
   <td>Protein Domain</td>
+
   <td>Translational Unit</td>
   <td><a href="http://partsregistry.org/Part:BBa_K322122">BBa_K322122</a></td>
   <td><a href="http://partsregistry.org/Part:BBa_K322122">BBa_K322122</a></td>
   <td>Harvard 2008's <a href="http://partsregistry.org/Part:BBa_K098010">BBa_K098010</a> in pSB1C3.</td>
   <td>Harvard 2008's <a href="http://partsregistry.org/Part:BBa_K098010">BBa_K098010</a> in pSB1C3.</td>
  </tr>
  </tr>
  <tr>
  <tr>
-
   <td align="left"><b>Phycobilin synthesis operon without terminator</b></td>
+
   <td align="left"><b>Phycocyanobilin synthesis operon without terminator</b></td>
-
   <td>Protein Domain</td>
+
   <td>Translational Unit</td>
   <td><a href="http://partsregistry.org/Part:BBa_K322123">BBa_K322123</a></td>
   <td><a href="http://partsregistry.org/Part:BBa_K322123">BBa_K322123</a></td>
   <td><a href="http://partsregistry.org/Part:BBa_K098010">BBa_K098010</a> without terminator, allowing addition of cph8.</td>
   <td><a href="http://partsregistry.org/Part:BBa_K098010">BBa_K098010</a> without terminator, allowing addition of cph8.</td>
  </tr>
  </tr>
  <tr>
  <tr>
-
   <td align="left"><b>cph8</b></td>
+
   <td align="left"><b>cph8 with RBS</b></td>
-
   <td>Protein Domain</td>
+
   <td>Translational Unit</td>
   <td><a href="http://partsregistry.org/Part:BBa_K322124">BBa_K322124</a></td>
   <td><a href="http://partsregistry.org/Part:BBa_K322124">BBa_K322124</a></td>
   <td>Light sensing protein (chimeric Cph1 light receptor / EnvZ protein) for red light.</td>
   <td>Light sensing protein (chimeric Cph1 light receptor / EnvZ protein) for red light.</td>
 +
</tr>
 +
<tr>
 +
  <td align="left"><b>cph8 and reporter system (lacZ)</b></td>
 +
  <td>Composite</td>
 +
  <td><a href="http://partsregistry.org/Part:BBa_K322125">BBa_K322125</a></td>
 +
  <td>Red light sensor with lacZ reporter system.</td>
 +
</tr>
 +
<tr>
 +
  <td align="left"><b>cph8 and reporter system (EYFP)</b></td>
 +
  <td>Composite</td>
 +
  <td><a href="http://partsregistry.org/Part:BBa_K322126">BBa_K322126</a></td>
 +
  <td>Red light sensor with EYFP reporter system.</td>
 +
</tr>
 +
<tr>
 +
  <td align="left"><b>Phycocyanobilin synthesis genes with cph8</b></td>
 +
  <td>Composite</td>
 +
  <td><a href="http://partsregistry.org/Part:BBa_K322127">BBa_K322127</a></td>
 +
  <td>The entire phycocyanobilin - cph8 system, without reporter system.</td>
 +
</tr>
 +
<tr>
 +
  <td align="left"><b>Phycocyanobilin synthesis genes with cph8 and EYFP reporter system</b></td>
 +
  <td>Composite</td>
 +
  <td><a href="http://partsregistry.org/Part:BBa_K322128">BBa_K322128</a></td>
 +
  <td>The entire phycocyanobilin - cph8 system, with EYFP reporter system.</td>
  </tr>
  </tr>
  <tr>
  <tr>
   <td align="left"><b>Firefly luciferase</b></td>
   <td align="left"><b>Firefly luciferase</b></td>
-
   <td>Protein Domain</td>
+
   <td>Coding</td>
-
   <td><a href="http://partsregistry.org/Part:BBa_K322124">BBa_K322124</a></td>
+
   <td><a href="http://partsregistry.org/Part:BBa_K322237">BBa_K322237</a></td>
-
   <td>Ljubljana 2007's <a href="http://partsregistry.org/Part:BBa_K322124">BBa_I712019</a> in pSB1C3.</td>
+
   <td>Ljubljana 2007's <a href="http://partsregistry.org/Part:BBa_I712019">BBa_I712019</a> in pSB1C3, from <i>Photinus pyralis</i>.</td>
  </tr>
  </tr>
  <tr>
  <tr>
   <td align="left"><b>Firefly luciferase S284T mutant</b></td>
   <td align="left"><b>Firefly luciferase S284T mutant</b></td>
-
   <td>Protein Domain</td>
+
   <td>Coding</td>
   <td><a href="http://partsregistry.org/Part:BBa_K322246">BBa_K322246</a></td>
   <td><a href="http://partsregistry.org/Part:BBa_K322246">BBa_K322246</a></td>
-
   <td>Firefly luciferase mutated towards the red spectrum.</td>
+
   <td>Firefly luciferase from <i>Photinus pyralis</i> mutated towards the red spectrum.</td>
  </tr>
  </tr>
  <tr>
  <tr>
   <td align="left"><b>Firefly luciferase 356K mutant</b></td>
   <td align="left"><b>Firefly luciferase 356K mutant</b></td>
-
   <td>Protein Domain</td>
+
   <td>Coding</td>
-
   <td><a href="http://partsregistry.org/Part:BBa_K322246">BBa_K322246</a></td>
+
   <td><a href="http://partsregistry.org/Part:BBa_K322211">BBa_K322211</a></td>
-
   <td>Firefly luciferase S284T mutant re-mutated towards the red spectrum.</td>
+
   <td>Firefly luciferase S284T mutant from <i>Photinus pyralis</i> re-mutated towards the red spectrum.</td>
  </tr>
  </tr>
  <tr>
  <tr>
Line 262: Line 286:
   <td>Coding</td>
   <td>Coding</td>
   <td><a href="http://partsregistry.org/Part:BBa_K322451">BBa_K322451</a></td>
   <td><a href="http://partsregistry.org/Part:BBa_K322451">BBa_K322451</a></td>
-
   <td>Photinus pyralis luciferase mutated for increased bioluminescence.</td>
+
   <td>Firefly luciferase from <i>Photinus pyralis</i> mutated for increased bioluminescence.</td>
  </tr>
  </tr>
</table>
</table>

Revision as of 16:36, 18 October 2010







BioBricks: Genomic BRIDGEs


For this part of the project we will have one main BioBrick, which will be the construct required for the two-step markerless insertion, encompassing cat (chloramphenicol resistance) and sacB (prevents growth on sucrose).

Chloramphenicol resistance has already been well characterised in the registry, so our characterisation has mainly focused on sacB.

In addition we will probably be submitting the construct with the up- and down- stream sequences of useful genes which can be removed, e.g. tnaA, which produces indole - when removed E. coli no longer smells!


Name Type Link Description
cat Translational Unit BBa_K322210 Chloramphenicol acetyltransferase (chloramphenicol resistance gene).
sacB Coding BBa_K322921 Bacillus subtilis levansucrase, lethal to E. coli in presence of sucrose.
tnaA upstream region Protein Domain BBa_K322705 Upstream region of E. coli tryptophanase locus, used for targetting genes to this locus using BRIDGE.
tnaA downstream region Protein Domain BBa_K322706 Downstream region of E. coli tryptophanase locus, used for targetting genes to this locus using BRIDGE.

Composite BioBricks in preparation.





BioBricks: Bacterial BRIDGEs


***


Name Type Link Description
luxAB Translational Unit BBa_K322139 Bacterial luciferase producing blue light (from X. luminescens).
luxCDE Protein Domain BBa_K322312 Codes for a fatty acid reductase complex that makes the fatty acids necessary for LuxAB.
lumP Translational Unit BBa_K322007 Lumazine protein to shift the wavelength of LuxAB to blue.
Phycocyanobilin synthesis operon Translational Unit BBa_K322122 Harvard 2008's BBa_K098010 in pSB1C3.
Phycocyanobilin synthesis operon without terminator Translational Unit BBa_K322123 BBa_K098010 without terminator, allowing addition of cph8.
cph8 with RBS Translational Unit BBa_K322124 Light sensing protein (chimeric Cph1 light receptor / EnvZ protein) for red light.
cph8 and reporter system (lacZ) Composite BBa_K322125 Red light sensor with lacZ reporter system.
cph8 and reporter system (EYFP) Composite BBa_K322126 Red light sensor with EYFP reporter system.
Phycocyanobilin synthesis genes with cph8 Composite BBa_K322127 The entire phycocyanobilin - cph8 system, without reporter system.
Phycocyanobilin synthesis genes with cph8 and EYFP reporter system Composite BBa_K322128 The entire phycocyanobilin - cph8 system, with EYFP reporter system.
Firefly luciferase Coding BBa_K322237 Ljubljana 2007's BBa_I712019 in pSB1C3, from Photinus pyralis.
Firefly luciferase S284T mutant Coding BBa_K322246 Firefly luciferase from Photinus pyralis mutated towards the red spectrum.
Firefly luciferase 356K mutant Coding BBa_K322211 Firefly luciferase S284T mutant from Photinus pyralis re-mutated towards the red spectrum.
Codon optimised bright firefly luciferase Coding BBa_K322451 Firefly luciferase from Photinus pyralis mutated for increased bioluminescence.

Composite BioBricks in preparation.