"
Team:Edinburgh/Bacterial/Red light producer
From 2010.igem.org
(Difference between revisions)
(New page: {{:Team:Edinburgh/defaultcss}} {{:Team:Edinburgh/defaultadvancedcss}} {{:Team:Edinburgh/dropdowncss}} <html> <head> <style type="text/css"> #content{ background-color: darkslategray; ...) |
|||
| Line 104: | Line 104: | ||
<br> | <br> | ||
| - | + | <p>Firefly luciferase (EC <a href="http://www.expasy.org/cgi-bin/nicezyme.pl?1.13.12.7">1.13.12.7</a>) from <a href="http://www.itis.gov/servlet/SingleRpt/SingleRpt?search_topic=TSN&search_value=722476" >Photinus pyralis</a> is one of the most efficient bioluminescent proteins known. Its emission peak is about 557nm at pH 7.8 (this is the ordinary internal pH of E. coli during growth). We attempted to produce a mutant luciferase which would produce red light, in order to activate the red light sensor part (which responds optimally to 660nm light).</p> | |
| + | |||
| + | <p>We used SDM on the wild type to produce different mutants:</p> | ||
| + | |||
| + | <ul> | ||
| + | <li>Substitution mutant S248T</li> | ||
| + | <li>Insertion mutant 356K</li> | ||
| + | <li>Insertion mutant 356R</li> | ||
| + | </ul> | ||
| + | |||
| + | <center><br><p><img src="https://static.igem.org/mediawiki/2010/d/d9/REDlucS248Tspectrum.jpg" width="442" height="368" border="0" /></p><br></center> | ||
| + | |||
| + | <center><br><p><img src="https://static.igem.org/mediawiki/2010/8/8e/REDluc356ins.jpg" width="450" height="323" border="0" /></p><br></center> | ||
<br> | <br> | ||
Revision as of 15:22, 27 July 2010
Firefly luciferase (EC 1.13.12.7) from Photinus pyralis is one of the most efficient bioluminescent proteins known. Its emission peak is about 557nm at pH 7.8 (this is the ordinary internal pH of E. coli during growth). We attempted to produce a mutant luciferase which would produce red light, in order to activate the red light sensor part (which responds optimally to 660nm light).
We used SDM on the wild type to produce different mutants:
- Substitution mutant S248T
- Insertion mutant 356K
- Insertion mutant 356R
