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Team:Edinburgh/Bacterial/Green light producer
From 2010.igem.org
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<p><a href="http://partsregistry.org/Part:BBa_K322238">BBa_K322238</a>: firefly luciferase from <i>Photinus pyralis</i> under <i>lac</i> promoter.</p> | <p><a href="http://partsregistry.org/Part:BBa_K322238">BBa_K322238</a>: firefly luciferase from <i>Photinus pyralis</i> under <i>lac</i> promoter.</p> | ||
<p><a href="http://partsregistry.org/Part:BBa_K322451">BBa_K322451</a>: firefly luciferase from <i>Photinus pyralis</i>, codon optimised and mutated for brighter bioluminescence.<br> | <p><a href="http://partsregistry.org/Part:BBa_K322451">BBa_K322451</a>: firefly luciferase from <i>Photinus pyralis</i>, codon optimised and mutated for brighter bioluminescence.<br> | ||
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| + | <a name="Characterisation" id="Characterisation"></a><h2>Characterisation</h2> | ||
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| + | <p>***</p> | ||
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Revision as of 16:34, 25 October 2010
Overview: The green light producer
The green luciferase will require the combination of luxAB with the production of another protein: YFP from ''Vibrio fischeri''. This will shift the wavelength of the light towards the yellow spectrum, making it the correct colour to activate our green light sensor. Mexico UNAM-Genomics have had this DNA sequence synthesised and sending it to us for characterisation and combination with luxAB. We also expect Mexico to BioBrick it as part of their submission to the registry.
Strategy
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Problems
You can't have problems with a piece of DNA you've never met.
BioBricks
Firefly luciferase was originally deposited in the Registry by Ljubljana 2007. We modified this slightly and re-submitted in pSB1C3, along with a codon-optimised mutant and a simple reporter system.
BBa_K322237: firefly luciferase from Photinus pyralis, modified BBa_I712019.
BBa_K322238: firefly luciferase from Photinus pyralis under lac promoter.
BBa_K322451: firefly luciferase from Photinus pyralis, codon optimised and mutated for brighter bioluminescence.
Characterisation
***
References
Fujii, H., Noda, K., Asami, Y., Kuroda, A., Sakata, M. & Tokida, A. (2007). Increase in bioluminescence intensity of firefly luciferase using genetic modification. Analytical Biochemistry 366, 131-136.
