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Team:ETHZ Basel/Modeling/Interworking
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== Tasks for our models== | == Tasks for our models== | ||
Revision as of 14:58, 22 September 2010
Evaluation for Wet Laboratory
Tasks for our models
1. Estimate CheYp for no tumbling: we already know from the literature that the tumbling frequency of the bacterium is a function of the concentration of CheY phosphorylated. Therefore, our first task was to determine the concentration of CheYp (defined as the ‘Threshold’) necessary to get a bias (= time not tumbling/total time) of 1 or close enough to 1 so that we could consider that the bacterium exhibits no tumbling (for a given input).
2. Construct the light input: Given the value of CheYp from 1), the second task would be to construct this light input which induces the effect of ‘no tumbling’ (or ‘running straight’). This is to be done by fusing PIF3 (for example) with any of the Che proteins (CheB, CheR, CheZ). The best light input signal is the one for which the bias is close enough to 1 for the highest amount of time (in other words, CheYp stays the most beyond the Threshold).
3. Repeat the previous tasks for different values of the input concentrations (different input values trigger different effects on the bias of the system).
