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| - | {{ETHZ_Basel10}}
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| - | {{ETHZ_Basel10_Modeling}}
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| - | = Evaluation for wet laboratory =
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| - | [[Image:ETHZ_Basel_models_overview_comb.png|thumb|400px|'''Combined models.''' Coupled individual models for the simulation of the whole process and their interfaces. The concentration of CheYp determines the movement bias.]]
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| - | == Goals of the evaluation ==
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| - | To determine the best possible parts for the biological implementation of E. lemming, the combined molecular model (Light switch - Chemotaxis) has been used to answer specific questions:
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| - | # Which receptor (Che) protein should be attacked?
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| - | # To which light-sensitive protein (PhyB, PIF3) should the receptor protein be linked?
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| - | # What is a good aspartate concentration?
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| - | # In what quantity should the anchor and binding light-sensitive protein be chosen?
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| - | Answers to this questions were able to decrease the experimental expenses significantly.
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| - | == Evaluator ==
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| - | [[Image:ETHZ_Basel_models_evaluator_amplitude.png|thumb|400px|'''Determination of relative amplitude.''' Relative amplitude of free CheYp was maximized to determine optimal parts.]]
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| - | Evaluation of the results is a general optimization problem. The output variable in the case of E. lemming is the concentration of free and phosphorylated CheYp, because it is directly linked to the movement bias. To achieve a reasonable evaluation variable, the relative amplitude of free and phosphorylated CheYp was chosen to be maximized.
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| - | Because of the limited amount of different combination possibilities (48), it was chosen to evaluate all combinations. Parameter space of variable experimental implementation possibilities is shown in Table 1.
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| - | {| border="1" align="center"
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| - | |+ '''Table 1: Experimental parameters'''
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| - | ! Che? !! LSP? !! LSP?' !! [Asp] !! [AP] !! [anchor]
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| - | |-
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| - | | CheR || PhyB || PIF3 || 0 uM || 0 uM || 0 uM
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| - | |-
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| - | | CheB || PIF3 || PhyB || 10^-6 uM || 25 uM || 25 uM
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| - | |-
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| - | | CheY |||||| 10^-3 uM || 50 uM || 50 uM
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| - | |-
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| - | | CheZ |||||||| 75 uM || 75 uM
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| - | |-
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| - | ||||||||| 100 uM || 100 uM
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| - | |}
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| - | == Results ==
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| - | = Evaluation for information processing =
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| - | == Goal of the evaluation ==
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| - | Main goal of the evaluation for information processing was to determine time constants of E. lemming by using the combined molecular model (Light switch - Chemotaxis).
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