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Revision as of 15:04, 6 August 2010 (view source) Luzi (Talk | contribs) (→testing of couplers) ← Older edit		Revision as of 15:05, 6 August 2010 (view source) Luzi (Talk | contribs) (→generation of biobricks) Newer edit →
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	= generation of biobricks =		= generation of biobricks =
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-	== testing of che-fusions ==
-	We're currently trying to establish an assay to test for the chemotactic behavour of ''E. coli''. A first try with a "syringe test" failed. We'll redo the test and are looking for other possibilites. Maybe a swarm test?
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-	== testing of localicer-fusions ==
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-	We are planning to use a fluorescence microscope in order to visualize the distribution of localizer-cyFP fusions within a single ''E. coli''.
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-	== testing of couplers ==
-	We're planning to use the setup as for the testing of the localizers. In this case we're going to use the following biobricks:
-	*localizer-PhyB and PIF3-cyFP or
-	*localizer-PIF3 and PhyB-cyFP
-	Images should look as in testing of localizer-fusions. Preliminary clarificaiton of the suitability of the approach are to be done.
	= testing of biobricks =		= testing of biobricks =

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Revision as of 15:05, 6 August 2010

generation of subparts

subparts

	Protein	primers	PCR product	library of transformants	single colonies	storage vector	insert
che proteins	CheY-A	recieved	purified on stock	on agar plate	not available	purified on stock	on stock
	CheY-C	recieved	purified in stock	on agar plate	not available	purified on stock	on stock
	CheB-A	recieved	purified in stock	on agar plate	3 on agar plate	purified on stock	on stock
	CheB-C	recieved	purified in stock	on agar plate	3 on agar plate	purified on stock	on stock
	CheR-A	recieved	purified in stock	on agar plate	3 on agar plate	digest has to be redone
	CheR-C	recieved	purified in stock	on agar plate	3 on agar plate	digest has to be redone
localizers	TrigF-A	recieved	purified in stock	on agar plate	3 on agar plate	purified on stock
	MreB-C	recieved	purified in stock	in process
	tetR-A	recieved	purified in stock	on agar plate	3 on agar plate	purified on stock	on stock
couplers	PIF3-A	recieved	purified in stock	on agar plate	3 on agar plate	purified on stock
	PIF3-C	recieved	purified in stock	on agar plate	3 on agar plate	purified on stock
	PhyB-C	recieved	purified in stock	on agar plate	3 on agar plate	purified on stock
reporters	cyFP-A	recieved	purified in stock	on agar plate	3 on agar plate	purified on stock	on stock
	cyFP-C	recieved	purified in stock	on agar plate	3 on agar plate	purified on stock

generation of biobricks

testing of biobricks

bringing E. lemming alive

timetable