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Team:ETHZ Basel/Biology/Status
From 2010.igem.org
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= generation of biobricks = | = generation of biobricks = | ||
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| + | == testing of che-fusions == | ||
| + | We're currently trying to establish an assay to test for the chemotactic behavour of ''E. coli''. A first try with a "syringe test" failed. We'll redo the test and are looking for other possibilites. Maybe a swarm test? | ||
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| + | == testing of localicer-fusions == | ||
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| + | == testing of couplers == | ||
= testing of biobricks = | = testing of biobricks = | ||
Revision as of 14:54, 6 August 2010
generation of subparts
| Protein | primers | PCR product | library of transformants | single colonies | storage vector | insert | |
|---|---|---|---|---|---|---|---|
| che proteins | CheY-A | recieved | purified on stock | on agar plate | not available | purified on stock | on stock |
| CheY-C | recieved | purified in stock | on agar plate | not available | purified on stock | on stock | |
| CheB-A | recieved | purified in stock | on agar plate | 3 on agar plate | purified on stock | on stock | |
| CheB-C | recieved | purified in stock | on agar plate | 3 on agar plate | purified on stock | on stock | |
| CheR-A | recieved | purified in stock | on agar plate | 3 on agar plate | digest has to be redone | ||
| CheR-C | recieved | purified in stock | on agar plate | 3 on agar plate | digest has to be redone | ||
| localizers | TrigF-A | recieved | purified in stock | on agar plate | 3 on agar plate | purified on stock | |
| MreB-C | recieved | purified in stock | in process | ||||
| tetR-A | recieved | purified in stock | on agar plate | 3 on agar plate | purified on stock | on stock | |
| couplers | PIF3-A | recieved | purified in stock | on agar plate | 3 on agar plate | purified on stock | |
| PIF3-C | recieved | purified in stock | on agar plate | 3 on agar plate | purified on stock | ||
| PhyB-C | recieved | purified in stock | on agar plate | 3 on agar plate | purified on stock | ||
| reporters | cyFP-A | recieved | purified in stock | on agar plate | 3 on agar plate | purified on stock | on stock |
| cyFP-C | recieved | purified in stock | on agar plate | 3 on agar plate | purified on stock |
generation of biobricks
testing of che-fusions
We're currently trying to establish an assay to test for the chemotactic behavour of E. coli. A first try with a "syringe test" failed. We'll redo the test and are looking for other possibilites. Maybe a swarm test?
testing of localicer-fusions
testing of couplers
testing of biobricks
bringing E. lemming alive
timetable
