"
Team:ETHZ Basel/Biology/Molecular Mechanism
From 2010.igem.org
Contents |
Relevant properties of the chemotaxis pathway
Localization Che proteins
In [1] CheA, CheY and CheZ proteins were fused to YFP in E. coli mutants to determine their localization inside the cell. All three proteins tend to co-localize with methyl accepting chemotaxis proteins (MCPs, the receptor proteins of the chemotaxis pathway) at the membrane, but with different strengths.
- CheA and CheZ nearly only localize at the MCPs.
- CheY has a higher concentration around the MCPs, but is present in significant concentrations in the cytoplasm.
Consistent with common knowledge, CheA was not localized inside the cell in mutants lacking MCPs. CheZ showed no localization in ΔcheA mutants. CheY did not show any significant localization in ΔcheA mutants nor in mutants lacking a significant amount of receptor proteins.
