Team:ESBS-Strasbourg/proteolux

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<span style="color:ivory;">Words written in <b><font color="#E9AF03">Yellow</font></b>
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The aim of our <b><i><a href="http://2010.igem.org/Team:ESBS-Strasbourg/Project"><font color="#E9AF03">
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<div class="heading">ProteOlux...</div>
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project</font></a></i></b> is to engineer a light-inducible degradation system as a new fundamental component that can be easily used to build more complex biological circuits inside chassis organisms. This new component consists of the bacterial protease ClpXP from Escherichia Coli fused to the photoreceptor protein phytochrome B of Arabidopsis thaliana. The degradation system is universally applicable to any given protein by addition of a specially designed Biobrick containing the phytochrome interacting factor (PIF3/6) and a specific degradation sequence (DAS-tag). This Biobrick can be added to the C-terminal of the target protein by standard assembly methods. Illumination of red light (660nm) induces a conformational change in phytochrome B and activates the system, an impulse of far-red light (730nm) leads to disruption of the degradation. This allows a tight control over the catalytic activity, enabling the modulation of protein function in a general fashion with the combined characteristics of specificity, high temporal precision and rapid reversibility.
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ESBS-Strasbourg Team
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<br><br>
 
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We are a student-run team from the trinational graduate school <i><font color="#8888FF">
 
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&quot;École supérieur de Biotechnologie Strasbourg&quot;</font></i> <a href="http://www-esbs.u-strasbg.fr"><font color="#E9AF03">
 
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(ESBS)</font></a> located in the capital of Europe - <b>Strasbourg, France.</b>
 
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The ESBS is a joint-venture of the Universities of Freiburg and Karlsruhe in
 
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Germany, the University of Bale in Switzerland and the University of Strasbourg
 
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in France. Students come from all four partner universities and furthermore from
 
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all around the three countries. </p>
 
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This is the second year that the <b>ESBS-Strasbourg</b> participates in the iGEM
 
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competition. Our team consists of <i>12 students</i> coming from different
 
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scientific backgrounds prior to the ESBS. This will help us to deal with the
 
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difficulties coming up during this interdisciplinary project. Our shared
 
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education at the ESBS however enables us to communicate more easily with each
 
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other. An overview of the people involved and our competences can be found on
 
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team</font></a></i></b> page.
 
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iGEM - INTERNATIONAL GENETICALLY ENGINEERED MACHINES
 
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<div class="desc">
 
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<b>iGEM</b> (international Genetically Engineered Machines) is a competition
 
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organized by MIT (Massachusetts Institute of Technology) in <b>Boston, USA</b>,
 
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since 2004 and has become one of the largest international competitions in the
 
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field of science. This year 128 teams of undergraduate students compete against
 
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each other, and for the second time <b>ESBS-Strasbourg</b> team is joining this
 
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competition.
 
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<br><br>
 
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<b>Synthetic biology</b> is a new area of biological research that combines <i>
 
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science</i> and <i>engineering</i>. Synthetic biology encompasses a variety of
 
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different approaches, methodologies and disciplines. We can define the synthetic
 
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biology as the <u>design</u> and<u> (re)construction</u> of new <u>biological
 
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functions</u> and <u>systems</u> not found in nature.
 
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Similar to the construction of a <b><i><font color="#8888FF">car</font></i></b>,
 
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Synthetic Biology uses simple <b><i> <font color="#8888FF">gene building blocks</font></i></b>
 
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for the construction of new complex <b> systems</b> with distinct functions.
 
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These gene building blocks are collected in a database by <b> iGEM</b> and can
 
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be used by all participants of the competition. So far, the collection contains
 
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13,000 gene building blocks</font></a></i>, due to the continuous development of
 
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“Jamboree” in Boston. Several prizes in different categories will be awarded.
 
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Revision as of 17:53, 29 November 2010

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