Team:ESBS-Strasbourg/Results/Characterization

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<div class="heading">PIF6-linker-GFP</div>
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In the course of our experiments, we were led to express PIF-linker-GFP to check if this fusion protein was fluorescent before going into further characterization of our system.<br>
In the course of our experiments, we were led to express PIF-linker-GFP to check if this fusion protein was fluorescent before going into further characterization of our system.<br>
We successfully expressed PIF6-linker-GFP under constitutive (promotor J23100 and RBS B0034) control (PIF3-linker-GFP is still in progress). As expected, the assembly of the parts works as predicted and neither the PIF6 nor the linker interfere with the GFP. It shows that those two factors could be assembled with any protein that would be targeted for degradation.<br>
We successfully expressed PIF6-linker-GFP under constitutive (promotor J23100 and RBS B0034) control (PIF3-linker-GFP is still in progress). As expected, the assembly of the parts works as predicted and neither the PIF6 nor the linker interfere with the GFP. It shows that those two factors could be assembled with any protein that would be targeted for degradation.<br>
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One really surprising result was that colonies expressing PIF6-linker-GFP were from far more fluorescent than those expressing GFP only. This came to a surprise since the expression of both proteins are controlled by the same promoter/RBS and that GFP-fusion proteins are more likely to be less fluorescent than GFP alone!</div>
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One really surprising result was that colonies expressing PIF6-linker-GFP were from far more fluorescent than those expressing GFP only. This came to a surprise since the expression of both proteins are controlled by the same promoter/RBS and that GFP-fusion proteins are more likely to be less fluorescent than GFP alone!
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Revision as of 22:02, 27 October 2010

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ESBS - Strasbourg



  

  
Let me guide you
PIF6-linker-GFP

In the course of our experiments, we were led to express PIF-linker-GFP to check if this fusion protein was fluorescent before going into further characterization of our system.
We successfully expressed PIF6-linker-GFP under constitutive (promotor J23100 and RBS B0034) control (PIF3-linker-GFP is still in progress). As expected, the assembly of the parts works as predicted and neither the PIF6 nor the linker interfere with the GFP. It shows that those two factors could be assembled with any protein that would be targeted for degradation.
One really surprising result was that colonies expressing PIF6-linker-GFP were from far more fluorescent than those expressing GFP only. This came to a surprise since the expression of both proteins are controlled by the same promoter/RBS and that GFP-fusion proteins are more likely to be less fluorescent than GFP alone!




  


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