Team:ESBS-Strasbourg/Results/Characterization
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In order to find an answer to this question, we calculated specific fluorescence by measuring both fluorescence and amount of fluorescent protein. First we measured fluorescences of three different E.coli lysats from one GFP negative strain, one GFP expressing strain and one PIF6-linker-GFP expressing strain. The table below shows the tests made on the previews tubes. Cells were resuspended and sonicated and the lysate was analyzed with a fluorimetre (absorption wavelenght=490nm, emission wavelenght=510nm). The test was done 3 times to give an average measurement. We then calculated the specific fluorescence according to the OD600, the results are also shown in the table.<br> | In order to find an answer to this question, we calculated specific fluorescence by measuring both fluorescence and amount of fluorescent protein. First we measured fluorescences of three different E.coli lysats from one GFP negative strain, one GFP expressing strain and one PIF6-linker-GFP expressing strain. The table below shows the tests made on the previews tubes. Cells were resuspended and sonicated and the lysate was analyzed with a fluorimetre (absorption wavelenght=490nm, emission wavelenght=510nm). The test was done 3 times to give an average measurement. We then calculated the specific fluorescence according to the OD600, the results are also shown in the table.<br> | ||
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Revision as of 23:04, 27 October 2010
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