Team:ESBS-Strasbourg/Project/Acknowledgment

From 2010.igem.org

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ESBS - Strasbourg
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</li>
</li>
<li>
<li>
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<p><br/><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Team">&nbsp;&nbsp;TEAM</a></p>
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<p><br/><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Team">
 +
&nbsp;&nbsp;TEAM</a></p>
<ul>
<ul>
-
<li><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Team">Overview</a></li>
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<li><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Team">
 +
Overview</a></li>
<li><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Team#under">
<li><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Team#under">
Students</a></li>
Students</a></li>
-
<li><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Team#instructors">
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<li><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Team#advisors">
Advisors</a></li>
Advisors</a></li>
 +
<li><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Team#instructors">
 +
Instructors</a></li>
<li><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Team#uni">
<li><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Team#uni">
Strasbourg</a></li>
Strasbourg</a></li>
 +
<li><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Team#collaboration">
 +
Collaboration</a></li>
                                
                                
</ul>
</ul>
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<li>
<li>
<p><br />
<p><br />
-
<a href="https://2010.igem.org/Team:ESBS-Strasbourg/Project">&nbsp;&nbsp;PROJECT</a></p>
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<a href="https://2010.igem.org/Team:ESBS-Strasbourg/Project">
 +
&nbsp;&nbsp;PROJECT</a></p>
<ul>
<ul>
-
<li><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Project">Overview</a></li>
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<li><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Project">
 +
Overview</a></li>
                                 <li><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Project/Strategy">
                                 <li><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Project/Strategy">
Strategy</a></li>
Strategy</a></li>
 +
                                <li><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Project/visual">
 +
Visual Description</a></li>
                                 <li><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Project/Application">
                                 <li><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Project/Application">
Application</a></li>
Application</a></li>
-
<li><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Project/Acknowledgment">Acknowledgment</a></li>
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<li><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Project/Acknowledgment">
 +
Acknowledgment</a></li>
<li><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Project/Reference">
<li><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Project/Reference">
Reference</a></li>
Reference</a></li>
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</li>
</li>
<li>
<li>
-
<p><br/><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Results">&nbsp;&nbsp;RESULTS</a></p>                 
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<p><br/><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Results/Biobricks">
 +
&nbsp;&nbsp;RESULTS</a></p>                 
                         <ul>
                         <ul>
-
<li><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Results/Biobricks">Biobricks</a></li>
+
<li><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Results/Biobricks">
 +
Biobricks</a></li>
 +
<li><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Results/Assembly">
 +
<font size="3">Biobrick Assembly Technique</font></a></li>
                                 <li><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Results/Characterization">
                                 <li><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Results/Characterization">
Characterization</a></li>
Characterization</a></li>
-
<li><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Results/Modelling">Modelling</a></li>
+
<li><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Results/Modelling">
 +
Modeling</a></li>
 +
 
</ul>
</ul>
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</li>
</li>
<li>
<li>
-
<p><br/><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Notebook">&nbsp;&nbsp;NOTEBOOK</a></p>
+
<p><br/><a>
 +
&nbsp;&nbsp;NOTEBOOK</a></p>
                         <ul>
                         <ul>
-
<li><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Notebook/Syntethic">Synthetic Photoreceptors</a></li>
+
<li><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Notebook/Syntethic">
 +
Synthetic Photoreceptors</a></li>
                                 <li><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Notebook/Microfluidics">
                                 <li><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Notebook/Microfluidics">
Microfluidics</a></li>
Microfluidics</a></li>
-
<li><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Notebook/Labbook">Lab-book</a></li>
+
                                <li><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Results/Device">Lighting device</a></li>
 +
<li><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Notebook/Labbook">
 +
Lab-book</a></li>
</ul>
</ul>
</li>
</li>
 +
 +
<li>
 +
<p><br/><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Humanpractice">
 +
HUMAN PRACTICE</a></p>
 +
                        <ul>
 +
<li><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Humanpractice#organisation">
 +
Organisation</a></li>
 +
<li><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Humanpractice#survey">
 +
Survey</a></li>
 +
                                <li><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Humanpractice#video">
 +
The ClpX video</a></li>
 +
                                <li><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Humanpractice#game">
 +
The ClpX game</a></li>
 +
<li><a href="https://2010.igem.org/Team:ESBS-Strasbourg/Humanpractice#safety">
 +
Project Safety</a></li>
 +
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</ul>
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</li>
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                 <li class="last">
<p><br />
<p><br />
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<a href="https://2010.igem.org/Team:ESBS-Strasbourg/Sponsors">&nbsp;&nbsp;SPONSORS</a></p>
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<a href="https://2010.igem.org/Team:ESBS-Strasbourg/Sponsors">
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;SPONSORS</a></p>
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<br>
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<div class="heading">ACKNOWLEDGEMENTS</div>
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<div class="heading">ACKNOWLEDGMENT</div>
<div class="desc">
<div class="desc">
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<br><br>
+
 
-
<a href="https://2010.igem.org/Team:ESBS-Strasbourg/Project/Strategy#degradation">1. Degradation system</a>
+
<b>Thank you:</b></a><br>
 +
<ul>
 +
<li><a href="#sibler">Pr. Annie-Paule Sibler</a></li>
 +
<li><a href="#bruno">Pr. Bruno Chatton</a></li>
 +
<li><a href="#kedinger">Pr. Claude Kedinger</a></li>
 +
<li><a href="#weiss">Pr. Etienne Weiss</a></li>
 +
<li><a href="#georges">Pr. Georges Orfanoudakis</a></li
 +
<li><a href="#peter">Pr. Peter Philippsen</a></li>
 +
<li><a href="#kircher">Pr. Stephan Kircher</a></li>
 +
<li><a href="#baker">Pr. Tania Baker</a></li>
 +
<li><a href="#weber">Pr. Wilfried Weber</a></li>
 +
</ul>
<br>
<br>
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<a href="https://2010.igem.org/Team:ESBS-Strasbourg/Project/Strategy#light">2. Light detection system</a>
+
 
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<td width="10" rowspan=65 bgcolor="#800000">
 +
</div>
 +
 
 +
<div id="windowbox" style="position:fixed; top:50%; left:20px; width:11%;">
 +
<span style="color:ivory;">
 +
&nbsp;&nbsp;
 +
<a href="https://2010.igem.org/Team:ESBS-Strasbourg/science">
 +
<img border="0" src="https://static.igem.org/mediawiki/2010/d/da/ESBS-Strasbourg-Clpx.gif" width="70" height="85" ></a>
<br>
<br>
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<a href="https://2010.igem.org/Team:ESBS-Strasbourg/Project/Strategy#tagging">3. Protein Tagging</a>
+
Let me guide you</span>
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<tr>
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<td width="750" bgcolor="#414141" height="100">
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<div class="heading">
 +
ESBS-Strasbourg would like to thank all the people who have generously provided their support:
 +
</div>
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</td>
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</tr>
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 +
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<td width="750" bgcolor="#800000" height="10">
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</td>
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<tr>
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<td width="750" height="220" bgcolor="#414141">
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<div class="name">
 +
<a name="sibler"></a>
 +
Pr. Annie-Paule Sibler
 +
</div>
 +
<div class="profiles">
 +
<img src="-" height="150" width="107">
 +
</div>
 +
 +
 +
<div class="desc">
 +
We thank Assistant-Professor Annie-Paule Sibler very much for her invaluable help regarding adjustments of protocols and troubleshooting. Special thank goes to her, for her extraordinary effort in rising up our lab and in supplying us with lab material.
<br>
<br>
-
<a href="https://2010.igem.org/Team:ESBS-Strasbourg/Project/Strategy#system">4. Light controllable protease</a>
+
<a href="http://immunobiotechnologie.u-strasbg.fr/fr/index.php">Visit Pr. Annie-Paule Sibler Lab's WebSite</font></a>
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<br><br>
+
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<a href="https://2010.igem.org/Team:ESBS-Strasbourg/notebook">Notebook</a>
+
</div>
</div>
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<td width="750" height="220" bgcolor="#414141">
 +
<div class="name">
 +
<a name="bruno"></a>
 +
Pr. Bruno Chatton
</div>
</div>
-
 
+
<div class="profiles">
-
<td width="750" bgcolor="#414141">
+
<img src="https://static.igem.org/mediawiki/2010/2/25/ESBS-Strasbourg-Bruno-Chatton.jpg" height="150" width="107">
 +
</div>
 +
 +
<div class="desc">
<div class="desc">
-
<div class="heading">ESBS-Strasbourg would like to thank all the people who have generously provided their support:</div>
+
We would like to thank Professor Bruno Chatton <a href="http://publicationslist.org/bchatton">(http://publicationslist.org/bchatton)</font></a> for his technical support in our project, his advises on our cloning strategy and general lab tricks. Your great help has been really appreciated.
<br>
<br>
-
<br><br>
+
<a href="http://transcription.u-strasbg.fr/">Visit Pr. Bruno Chatton Lab's WebSite</font></a>
-
<b><a href="http://web.mit.edu/bakerlab/pages/people.html"<font size="2">Dr. Tania Baker</a></b> for her kind donation of ClpX (-) E.Coli strains and her help in resolving some problems.
+
</div>
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<br><br>
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blabla</div>
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<tr>
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<td width="750" height="220" bgcolor="#414141">
 +
<div class="name">
 +
<a name="kedinger"></a>
 +
Pr. Claude Kedinger
 +
</div>
 +
<div class="profiles">
 +
<img src="https://static.igem.org/mediawiki/2010/f/fa/ESBS-Strasbourg-Claude-Kedinger.jpg" height="150" width="107">
 +
</div>
 +
 +
 +
<div class="desc">
 +
We are thankful to Professor Claude Kedinger, Dean of the ESBS, for standing firmly behind the team concerning all project aspects and to the ESBS administration (Lydie Habillon, Brigitte Daul, Clair Guth and Monique Maurer) for their considerable help.
<br>
<br>
 +
<a href="http://www-esbs.u-strasbg.fr/esbs/?page_id=216&lang=en">Visit Pr. Claude Kedinger Lab's WebSite</font></a>
 +
</div>
</td>
</td>
</tr>
</tr>
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 +
<tr>
<tr>
-
<td width="750" height="10" bgcolor="#222222">
+
<td width="750" height="10" bgcolor="#222222"></td>
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</tr>
 +
 +
<tr>
 +
<td width="750" height="220" bgcolor="#414141">
 +
<div class="name">
 +
<a name="weiss"></a>
 +
Pr. Etienne Weiss
 +
</div>
 +
<div class="profiles">
 +
<img src="-" height="150" width="107">
 +
</div>
 +
 
 +
 +
<div class="desc">
 +
We have been appreciative for your advise and technical support for the characterization of PIF6-Linker-GFP.
 +
<br>
 +
<a href="http://immunobiotechnologie.u-strasbg.fr/fr/index.php">Visit Pr. Etienne Weiss Lab' WebSite</font></a>
 +
</div>
</td>
</td>
</tr>
</tr>
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 +
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<tr>
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<td width="750" height="10" bgcolor="#222222"></td>
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</tr>
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 +
<tr>
 +
<td width="750" height="220" bgcolor="#414141">
 +
<div class="name">
 +
<a name="georges"></a>
 +
Pr. Georges Orfanoudakis
 +
</div>
 +
<div class="profiles">
 +
<img src="https://static.igem.org/mediawiki/2010/3/36/ESBS-Strasbourg-orfanoudakis.jpg" height="150" width="107">
 +
</div>
 +
 +
 +
<div class="desc">
 +
We are grateful to Professor Georges Orfanoudakis for his advises on protein expression and purification experiments and for his material support concerning this matter.
 +
<br>
 +
<a href="http://oncoproteines.u-strasbg.fr/fr/index.php">Visit Pr. Georges Orfanoudakis Lab's WebSite</font></a>
 +
</div>
 +
</td>
 +
</tr>
 +
 +
<tr>
<tr>
-
<td width="750" bgcolor="#414141"">
+
<td width="750" height="10" bgcolor="#222222"></td>
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</tr>
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 +
<tr>
 +
<td width="750" height="220" bgcolor="#414141">
 +
<div class="name">
 +
<a name="peter"></a>
 +
Pr. Peter Philippsen
 +
</div>
 +
<div class="profiles">
 +
<img src="https://static.igem.org/mediawiki/2010/0/0c/ESBS-Strasbourg-Philippsen.jpg" height="150" width="107">
 +
</div>
 +
 
 +
 +
<div class="desc">
 +
We thank Professor Peter Philippsen for his constant support to ESBS iGEM Teams. We appreciated your lab material gift as well as the Swiss chocolate.
<br>
<br>
-
<div class="heading">
+
<a href="http://www.biozentrum.unibas.ch/philippsen/index.html">Visit Pr. Peter Philippsen Lab's WebSite</font></a>
-
Abstract
+
</div>
</div>
 +
</td>
 +
</tr>
 +
 +
 +
 +
<tr>
 +
<td width="750" height="10" bgcolor="#222222"></td>
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</tr>
 +
 +
<tr>
 +
<td width="750" height="220" bgcolor="#414141">
 +
<div class="name">
 +
<a name="kircher"></a>
 +
Pr. Stephan Kircher
 +
</div>
 +
<div class="profiles">
 +
<img src="-" height="150" width="107">
 +
</div>
 +
 +
<div class="desc">
<div class="desc">
 +
We are regardful to Professor Stephan Kircher (University of Freiburg, Germany) for lending a red and far red light LED interface to us for our characterization experiments and giving us the PIF3 gene.
<br>
<br>
-
<br>
+
<a href="http://www.biologie.uni-freiburg.de/data/bio2/schaefer/AG-Kircher/index.htm">Visit Pr. Stephan Kircher Lab's WebSite</font></a>
-
The aim of our project is to engineer a new fundamental component that could be universally used to build more complex or more controllable biological circuits inside chassis organisms. This new component consists of the E.coli protease ClpXP to which the phytochrome B of arabidopsis thaliana is fused. Any given protein can be degraded as long as it is tagged with our especially designed Biobrick containing a PIF sequence. This Biobrick can be added to the C-terminal end by standard assembly methods. The activity of this system is tightly controlled and reversible by light inducement.
+
-
<br><br>
+
-
The system can be constitutively expressed in the chassis but remains  inactive for the tagged protein. However it is expected to stay active for the background of naturally SsrA tagged proteins and so it will not interfere with the metabolism of E.coli. Instantly after the light inducement the system is turned on, due to the lack of transcriptional or translational delay and is expected to remain active until another light signal turns it off.
+
-
<br><br>
+
-
In our modeling part we will used this system for a genetic oscillator which could be used for multi step synthesis.
+
-
<br><br>
+
-
<p><b>Theoretical Background:</b></p>
+
-
<br>
+
-
Our system combines two main components: the ClpXP protease and the phytochrome B/ PIF system The ClpXP component is important for the specificity and the phytochrome B/ PIF system is important for the light sensitivity.
+
-
<br>
+
-
<br>
+
-
-Specificity : We based our system on the ClpXP protease, from E. coli. In wild type E. coli, ClpX forms a hexametric ring and binds to a double heptamer of ClpP. ClpX recognizes a specific C-terminal degradation tag called SsrA and starts to unfold the tagged protein. The denatured polypeptide is translocated into the ClpP subunit, which breaks down peptide bonds.
+
-
<br>
+
-
<br>
+
-
Note : In the native organism, the SsrA tag is added to incomplete proteins whose translation has been aborted. Thus, misfunctionnal proteins do not accumulate inside the cell.
+
-
<br>
+
-
<br>
+
-
-Light sensitivity : We used PhyB (phytochrome B) from A. thaliana and its natural chromophore PCB (phycocyanobilline). This system has two configurations. The Pf configuration which describes the inactive state of the PhyB and the Pfr configuration which describes the active state. The PCB of the holo phytochrome can absorb red light (pick at 660 nm) and through a structural transition it reaches its activated Pfr state. Under far red light (730 nm) the holo phytochrome goes back to its fundamental inactive Pr state. PIF-3 and PIF-6 (Phytochrome Interacting Factor 3 and 6) are natural partners of PhyB in A. thaliana and bind to PhyB in the Pfr state but not in the Pr state. This is the basis of the controllable feature of our system.
+
-
<br>
+
-
<br><br>
+
-
<p><b>How does the native ClpX system work?</b></p>
+
-
<br>
+
-
There are different variant of SsrA tags which are more or less efficiently recognized by ClpX. The DAS tag (T.Baker et al.) has a higher Kd value than wild type SsrA tag, and thus degradation of DAS-tagged proteins is not significant.
+
-
<br>
+
-
<br>
+
-
In vivo though, DAS-tagged protein can still be significantly degraded within the range of physiological concentrations under the action of an adaptator protein (SspB) which help tethering the tagged protein to the protease.
+
-
<br>
+
-
<br>
+
-
Our idea is to replace this adaptator system with a light sensitive tethering system.
+
-
<br>
+
-
<br><br>
+
-
<p><b>How did we proceed?</b></p>
+
-
<br>
+
-
For the protease, we fused either the 642 or 908 first amino acids of PhyB to a trimer of the last 364 amino acids of ClpX. Indeed, the N terminal end of ClpX contains a domain, which is responsible for interaction with its natural adaptator protein (SspB) that we would avoid. As this N-terminal domain is also partially responsible for ClpX subunits complexation into an hexamer, fusing three C-terminal end of ClpX together with appropriate linkers increases the stability of the system (T. Baker et al.) in the absence of this N-terminal domain.
+
-
<br>
+
-
<br>
+
-
The target protein will be tagged with both DAS at C-terminal end, and with the Active Phytochrome Binding (APB) motif (the first hundred amino acids of PIF-3 or PIF-6), either at N terminal end or just upstream the DAS tag.
+
-
<br>
+
-
<br>
+
-
Under red light, the APB motif of the target protein binds to PhyB and facilitate recognition of DAS tag by ClpX, thus promoting degradation.
+
-
Far red light instantly inhibits degradation of further tagged proteins.
+
-
<br>
+
-
<br>
+
</div>
</div>
</td>
</td>
</tr>
</tr>
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 +
<tr>
 +
<td width="750" height="10" bgcolor="#222222"></td>
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 +
<tr>
 +
<td width="750" height="220" bgcolor="#414141">
 +
<div class="name">
 +
<a name="baker"></a>
 +
Dr. Tania Baker
 +
</div>
 +
<div class="profiles">
 +
<img src="https://static.igem.org/mediawiki/2010/5/58/Tania_baker.jpg" height="150" width="107">
 +
</div>
 +
 +
 +
<div class="desc">
 +
The interactions with Professor Tania A. Baker (MIT, USA) on the light inducible system have been very beneficial. We would like to thank for her multiple responses and her experimental support by sending to us two different E. coli ClpX knock-out strains.
 +
<br>
 +
<a href="http://web.mit.edu/bakerlab/index.html">Visit The Baker's Lab WebSite</font></a>
 +
 +
</div>
 +
</td>
 +
</tr>
 +
 +
<tr>
 +
<td width="750" height="10" bgcolor="#222222"></td>
 +
</tr>
 +
 +
<tr>
 +
<td width="750" height="220" bgcolor="#414141">
 +
<div class="name">
 +
<a name="weber"></a>
 +
Dr. Wilfried Weber
 +
</div>
 +
<div class="profiles">
 +
<img src="https://static.igem.org/mediawiki/2010/b/bd/Wilfried_Weber.jpg" height="150" width="107">
 +
</div>
 +
 +
 +
<div class="desc">
 +
We like to thank Professor Wilfried Weber (BIOSS Freiburg, Germany) for taking the time to meet with us, for giving us advices on our project and for supporting us with the PIF6 and the phytochrome genes and the PCB chromophore extract.
 +
<br>
 +
<a href="http://www.bioss.uni-freiburg.de/cms/weber.html">Visit Pr. Wilfried Weber Lab's WebSite</font></a>
 +
</div>
 +
</td>
 +
</tr>
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Latest revision as of 17:02, 27 October 2010

{|

ESBS - Strasbourg



ACKNOWLEDGMENT
Thank you:
  
Let me guide you
ESBS-Strasbourg would like to thank all the people who have generously provided their support:
Pr. Annie-Paule Sibler
We thank Assistant-Professor Annie-Paule Sibler very much for her invaluable help regarding adjustments of protocols and troubleshooting. Special thank goes to her, for her extraordinary effort in rising up our lab and in supplying us with lab material.
Visit Pr. Annie-Paule Sibler Lab's WebSite
Pr. Bruno Chatton
We would like to thank Professor Bruno Chatton (http://publicationslist.org/bchatton) for his technical support in our project, his advises on our cloning strategy and general lab tricks. Your great help has been really appreciated.
Visit Pr. Bruno Chatton Lab's WebSite
Pr. Claude Kedinger
We are thankful to Professor Claude Kedinger, Dean of the ESBS, for standing firmly behind the team concerning all project aspects and to the ESBS administration (Lydie Habillon, Brigitte Daul, Clair Guth and Monique Maurer) for their considerable help.
Visit Pr. Claude Kedinger Lab's WebSite
Pr. Etienne Weiss
We have been appreciative for your advise and technical support for the characterization of PIF6-Linker-GFP.
Visit Pr. Etienne Weiss Lab' WebSite
Pr. Georges Orfanoudakis
We are grateful to Professor Georges Orfanoudakis for his advises on protein expression and purification experiments and for his material support concerning this matter.
Visit Pr. Georges Orfanoudakis Lab's WebSite
Pr. Peter Philippsen
We thank Professor Peter Philippsen for his constant support to ESBS iGEM Teams. We appreciated your lab material gift as well as the Swiss chocolate.
Visit Pr. Peter Philippsen Lab's WebSite
Pr. Stephan Kircher
We are regardful to Professor Stephan Kircher (University of Freiburg, Germany) for lending a red and far red light LED interface to us for our characterization experiments and giving us the PIF3 gene.
Visit Pr. Stephan Kircher Lab's WebSite
Dr. Tania Baker
The interactions with Professor Tania A. Baker (MIT, USA) on the light inducible system have been very beneficial. We would like to thank for her multiple responses and her experimental support by sending to us two different E. coli ClpX knock-out strains.
Visit The Baker's Lab WebSite
Dr. Wilfried Weber
We like to thank Professor Wilfried Weber (BIOSS Freiburg, Germany) for taking the time to meet with us, for giving us advices on our project and for supporting us with the PIF6 and the phytochrome genes and the PCB chromophore extract.
Visit Pr. Wilfried Weber Lab's WebSite

Retrieved from "http://2010.igem.org/Team:ESBS-Strasbourg/Project/Acknowledgment"