Here's a list of papers from which we got some ideas / inspiration and useful information

Asaia related

• Bacteria of the Genus Asaia: A Potential Paratransgenic Weapon Against Malaria

• Bacteria of the genus Asaia stably associate with Anopheles stephensi, an Asian malarial mosquito vector isolation of Asaia from mosquitoes and expression of GFP

• Paternal transmission of symbiotic bacteria in malaria vectors Asaia transmission and stable Asaia mCherry strain

• Cloning of Escherichia coli lacZ and lacY Genes and Their Expression in Gluconobacter oxydans and Acetobacter liquefaciens Asaia plasmid

Blocking Plasmodium falciparum transmission

Bacteria expressing single-chain immunotoxin inhibit malaria parasite development in mosquitoes Immunotoxin against malaria parasite, composed of Shiva-1 and mouse single-chain antibody fragment against Pbs21. A single-chain antibody fragment specific for the Plasmodium berghei ookinete protein Pbs21 confers transmission blockade in the mosquito midgut 13.1 scFv single-chain antibody fragment against ookinete protein Pbs21 In vitro cytocidal effect of novel lytic peptides on Plasmodium falciparum and Trypanosoma cruzi Shiva-1 Ajay K. Saxena, Yimin Wu, and David N. Garboczi, Plasmodium P25 and P28 Surface Proteins: Potential Transmission-Blocking Vaccines, Eukaryot. Cell, Aug 2007; 6: 1260 - 1265 Link. Using antibodies to bind both ookinete surface proteins P25 and P28 could prevent the epithelial invasion.

Ideas

1. Engineering BioBrick vectors from BioBrick parts This might help us to make an Asaia plasmid?