Team:EPF Lausanne/Project literature
From 2010.igem.org
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# [http://linkinghub.elsevier.com/retrieve/pii/S016668510000387X Bacteria expressing single-chain immunotoxin inhibit malaria parasite development in mosquitoes] Immunotoxin against malaria parasite, composed of Shiva-1 and mouse single-chain antibody fragment against Pbs21. Expressed in E. coli, but in inclusion bodies (might be a problem for us!!!). Inclusion bodies were solubilized, refolded and purified and then shown to inhibit ookinete development. | # [http://linkinghub.elsevier.com/retrieve/pii/S016668510000387X Bacteria expressing single-chain immunotoxin inhibit malaria parasite development in mosquitoes] Immunotoxin against malaria parasite, composed of Shiva-1 and mouse single-chain antibody fragment against Pbs21. Expressed in E. coli, but in inclusion bodies (might be a problem for us!!!). Inclusion bodies were solubilized, refolded and purified and then shown to inhibit ookinete development. |
Revision as of 16:48, 25 October 2010
Here's a list of papers from which we got some ideas / inspiration and useful information
- Bacteria of the Genus Asaia: A Potential Paratransgenic Weapon Against Malaria I (Henrike) did not manage to get this paper... could anybody send it to me please?
- Bacteria of the genus Asaia stably associate with Anopheles stephensi, an Asian malarial mosquito vector isolation of Asaia from mosquitoes and expression of GFP
- Paternal transmission of symbiotic bacteria in malaria vectors Asaia transmission and stable Asaia mCherry strain
- Cloning of Escherichia coli lacZ and lacY Genes and Their Expression in Gluconobacter oxydans and Acetobacter liquefaciens Asaia plasmid
Blocking Plasmodium falciparum transmission
- Bacteria expressing single-chain immunotoxin inhibit malaria parasite development in mosquitoes Immunotoxin against malaria parasite, composed of Shiva-1 and mouse single-chain antibody fragment against Pbs21. Expressed in E. coli, but in inclusion bodies (might be a problem for us!!!). Inclusion bodies were solubilized, refolded and purified and then shown to inhibit ookinete development.
- A single-chain antibody fragment specific for the Plasmodium berghei ookinete protein Pbs21 confers transmission blockade in the mosquito midgut 13.1 scFv single-chain antibody fragment against ookinete protein Pbs21
- In vitro cytocidal effect of novel lytic peptides on Plasmodium falciparum and Trypanosoma cruzi Shiva-1
- Ajay K. Saxena, Yimin Wu, and David N. Garboczi, Plasmodium P25 and P28 Surface Proteins: Potential Transmission-Blocking Vaccines, Eukaryot. Cell, Aug 2007; 6: 1260 - 1265 Link. Using antibodies to bind both ookinete surface proteins P25 and P28 could prevent the epithelial invasion.
Ideas
- Engineering BioBrick vectors from BioBrick parts This might help us to make an Asaia plasmid?