Team:Debrecen-Hungary/protocols
From 2010.igem.org
Welcome To Our Notebook"Science is nothing else but the art of proper reproducibility. " ContentsNotebook protocols utilized by the bacterial work subteam Making Lurea's Broth - Transformation of competent cells - Mini Prep Notebook protocols utilized by the molecular tools subteam Restriction enzyme digestion - PCR Purification - Gel electrophoresis - Gel purification Notebook protocols utilized by the tissue culture subteam Cell Passaging - Media PEI Preparation - Transfection - Ligand Treatment Notebook protocols utilized by the Luciferase subteam Measuring Luciferase activity with the Victor plate reader Notebook protocols utilized by the bacterial work subteamMaking Lurea's BrothTransformation of competent cellsMini PrepNotebook protocols utilized by the molecular tools subteamRestriction enzyme digestionPCR product purificationGel electrophoresisGel purificationNotebook protocols utilized by the tissue culture subteamCell PassagingMedia PEI PreparationTransfectionLigand Treatment |