Team:Debrecen-Hungary/protocols

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(Transformation of competent cells)
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=== Transformation of competent cells ===
=== Transformation of competent cells ===
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Transformation is the process of introducing foreign DNA (e.g plasmids, BAC) into a bacterium. Bacterial cells into which foreign DNA can be transformed are called competent. Some bacteria are naturally competent (e.g B. subtilis), whereas others such as E. coli are not naturally competent. Non-competent cells can be made competent and then transformed via one of two main approaches; chemical transformation and electroporation. It is important to note we have tested transformations of the distribution kit with this protocol. We have found that it is the best protocol. This protocol may be particularly useful if you are finding that your transformations are not working or yiedling few colonies.[http://www.openwetware.org/wiki/IGEM:University_of_Debrecen:Transformation_of_competent_cells Read more...]
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Transformation is the process of introducing foreign DNA (e.g plasmids, BAC) into a bacterium. Bacterial cells into which foreign DNA can be transformed are called competent. Some bacteria are naturally competent (e.g B. subtilis), whereas others such as E. coli are not naturally competent. Non-competent cells can be made competent and then transformed via one of two main approaches; chemical transformation and electroporation. It is important to note we have tested transformations of the distribution kit with this protocol.[http://www.openwetware.org/wiki/IGEM:University_of_Debrecen:Transformation_of_competent_cells Read more...]
=== Mini Prep ===
=== Mini Prep ===

Revision as of 15:15, 28 September 2010

Welcome To Our Notebook

"Science is nothing else but the art of proper reproducibility. "


The iGEM experience is not merely a project or a conference, but it was the way that most of our students got acquainted with the world of biological research laboratory. Pipettes, solutions, gels, electrodes, dishes and other scary machinery quickly filled our lives. From day one we saw the vast importance of teaching our students to keep a proper laboratory journal. As time passed and our project grew., and with growth sprouted the idea of keeping an electronic laboratory journal with texts and video’s depicting the proper way of doing our niche of science. And so, we present to you our combined effort a text and video version

Contents

Notebook protocols utilized by the bacterial work subteam

Making Lurea's Broth - Transformation of competent cells - Mini Prep

Notebook protocols utilized by the molecular tools subteam

Restriction enzyme digestion - PCR Purification - Gel electrophoresis - Gel purification

Notebook protocols utilized by the tissue culture subteam

Cell Passaging - Media PEI Preparation - Transfection - Ligand Treatment

Notebook protocols utilized by the Luciferase subteam

Measuring Luciferase activity with the Victor plate reader

Notebook protocols utilized by the bacterial work subteam

Making Lurea's Broth

Read more...

Transformation of competent cells

Transformation is the process of introducing foreign DNA (e.g plasmids, BAC) into a bacterium. Bacterial cells into which foreign DNA can be transformed are called competent. Some bacteria are naturally competent (e.g B. subtilis), whereas others such as E. coli are not naturally competent. Non-competent cells can be made competent and then transformed via one of two main approaches; chemical transformation and electroporation. It is important to note we have tested transformations of the distribution kit with this protocol.Read more...

Mini Prep

Read more...

Notebook protocols utilized by the molecular tools subteam

Restriction enzyme digestion

PCR product purification

Gel electrophoresis

Gel purification

Notebook protocols utilized by the tissue culture subteam

Cell Passaging

Media PEI Preparation

Transfection

Ligand Treatment

Notebook protocols utilized by the Luciferase subteam

Measuring Luciferase activity with the Victor plate reader