Revision as of 13:47, 28 September 2010

Welcome To Our Notebook

"Science is nothing else but the art of proper reproducibility. "
The iGEM experience is not merely a project or a conference, but it was the way that most of our students got acquainted with the world of biological research laboratory. Pipettes, solutions, gels, electrodes, dishes and other scary machinery quickly filled our lives. From day one we saw the vast importance of teaching our students to keep a proper laboratory journal. As time passed and our project grew., and with growth sprouted the idea of keeping an electronic laboratory journal with texts and video’s depicting the proper way of doing our niche of science. And so, we present to you our combined effort a text and video version

Notebook protocols utilized by the bacterial work subteam

Making Lurea's Broth

Transformation of competent cells

Mini Prep

Notebook protocols utilized by the molecular tools subteam

Restriction enzyme digestion

PCR product purification

Gel electrophoresis

Gel purification

Notebook protocols utilized by the tissue culture subteam

Cell Passaging

Media PEI Preparation

Transfection

Ligand_Treatment

@@ Line 32: / Line 32: @@
+==  Notebook protocols utilized by the bacterial work subteam  ==
+=== Making Lurea's Broth===
+=== Transformation of competent cells ===
+=== Mini Prep ===
+== Notebook protocols utilized by the molecular tools subteam ==
+=== Restriction enzyme digestion ===
+=== PCR product purification ===
+=== Gel electrophoresis ===
+=== Gel purification ===
+== Notebook protocols utilized by the tissue culture subteam ==
+=== Cell Passaging ===
+=== Media PEI Preparation ===
+=== Transfection ===
+=== Ligand_Treatment ===
 </div>

Team:Debrecen-Hungary/protocols

From 2010.igem.org