Team:Davidson-MissouriW/Tools

From 2010.igem.org

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         <div id="mission_box"> <h2> iGEM Davidson – Missouri Western 2010: Tools </h2>
         <div id="mission_box"> <h2> iGEM Davidson – Missouri Western 2010: Tools </h2>
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             <h3>The team has created several tools in conjunction with our iGem project.</h3>
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             <h3>The team has designed several tools to guide and support our iGEM project.</h3>
         </div>
         </div>
         <br><br><br><br><br><br>
         <br><br><br><br><br><br>
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         <div id="Oligator">
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         <div id="Veripart">
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             <center>
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             <a href="http://72.22.219.205/sequence">
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                <a href="http://gcat.davidson.edu/igem10/index.html"></a>
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                 <h3>VeriPart</h3>
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            </center>
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             </a><a NAME="veripart">
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            <a href="http://gcat.davidson.edu/igem10/index.html">
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                 <h3>The Oligator</h3>
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             </a><a NAME="Oligator">
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             <table cellpadding=0 cellspacing=0>
             <table cellpadding=0 cellspacing=0>
                 <tbody>
                 <tbody>
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                         </td>
                         </td>
                         <td width=580>
                         <td width=580>
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                             <p>Designing and building DNA sequences is a fundamental tool for anyone who works in synthetic biologyThe Oligator is designed to choose oligos to create any desired sequence between 20 and 20,000 base pairs.  The program allows the user to choose oligo lengths between 20 and 160 bp and overlap lengths of between 20 and 80 bp. 
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                             <p>Verifying BioBrick parts can be difficult and tedious by hand.  To solve this problem, we have developed a tool to identify BioBrick parts within a given DNA sequenceWhen a sequence is entered, the program checks the last archived version of the BioBrick Parts Registry and returns the best matched part. If your desired part is not found, you can attempt to align the sequence you entered with the part you desire. When a device is found in your sequence, you can click to view the sequences of individual parts. An alignment of the sequence with the part is presented to the user allowing for easier sequence analysis.</p>  
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The program will also check for and remove BioBrick restriction sites at the request of the user. Choosing the first checkbox will tell the user if BioBrick restriction sites exist anywhere in the entered DNA sequence and embolden where they occur. The second checkbox will remove the sites, leaving the amino acid sequence intact. Since keeping the amino acid sequence the same involves knowing the reading frame, a radio button must be filled in indicating where the reading frame begins.
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The final option is to add BioBrick ends to your sequence. This is helpful when cloning your sequence into a BioBrick plasmid. Simply choose which site you want to add to the 5' end. The options are a prefix digested with XbaI, a prefix digested with EcoRI or the entire prefix. You then choose which site you want to add to the 3' end. The options are a suffix digested with SpeI, a suffix digested with PstI or the entire suffix. However, if you choose to add a prefix, you must choose a suffix, and vice-versa. Furthermore, you also have the ability to add a custom prefix and suffix.</p>
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                         </td>
                         </td>
                         <td align=center width=600>
                         <td align=center width=600>
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                            <img src="http://2010.igem.org/wiki/images/3/33/Davidson-MoWOligator.png" width=400 height=305 alt="gator"/>
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                <a href="http://72.22.219.205/sequence"><img src="http://2010.igem.org/wiki/images/3/32/Davidson-MissouriWveripart.png" alt="Veri" width=400/></a>
                         </td>
                         </td>
                     </tr>
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         </div>
         </div>
         <br><br>
         <br><br>
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         <div id="Optimoose">  
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         <div id="Oligator">
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             <center>
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             <a href="http://gcat.davidson.edu/igem10/index.html">
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                <a href="http://gcat.davidson.edu/igem10/opt/opt_index.html"></a>
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                 <h3>The Oligator</h3>
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            </center>
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             </a><a NAME="Oligator">
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            <a href="http://gcat.davidson.edu/igem10/opt/opt_index.html">
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                 <h3>The Optimoose</h3>
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             </a><a NAME="optimoose">
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             <table cellpadding=0 cellspacing=0>
             <table cellpadding=0 cellspacing=0>
                 <tbody>
                 <tbody>
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                         </td>
                         </td>
                         <td width=580>
                         <td width=580>
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                             <p>The Optimoose is a tool designed to allow the user to evaluate the expression level of a sequence from ecoli using either RCBS-PC or the CAI formula.  The user has then the option to either optimized or deoptimized the sequence by using one of those two formulas. If the optimize or deoptimize option is selected the user will be given a new sequence in which the codons have been changed to reflect the best, or worst, codons as determined by the selected formula. However, the amino acid sequence is preserved.
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                             <p>Designing and building DNA sequences is a fundamental tool for anyone who works in synthetic biology.  The Oligator is designed to choose oligos to build any desired sequence between 20 and 20,000 base pairs.  The program allows the user to choose oligo lengths between 20 and 160 bp and overlap lengths of between 20 and 80 bp.
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The Optimoose was designed to allow us to assign a given weight to the items to fill up the knapsack. Based on how optimized or deoptimized the sequence is , and based on the capacity of the knapsack; we could give every items a weight to satisfy our goals.</p>
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The program will optionally check for and remove BioBrick restriction sites. Choosing the first checkbox will tell the user if BioBrick restriction sites exist anywhere in the entered DNA sequence and highlight where they occur. The second checkbox will remove the sites, leaving the amino acid sequence intact, based on the user-input reading frame. Since keeping the amino acid sequence the same involves knowing the reading frame, a radio button must be filled in indicating where the reading frame begins.
 +
The final option is to add BioBrick ends to your sequence. This is helpful when cloning your sequence into a BioBrick plasmid. Simply choose which site you want to add to the 5' end. The options are a prefix digested with XbaI, a prefix digested with EcoRI or the entire prefix. You then choose which site you want to add to the 3' end. The options are a suffix digested with SpeI, a suffix digested with PstI or the entire suffix. However, if you choose to add a prefix, you must choose a suffix, and vice-versa. Furthermore, you also have the ability to add a custom prefix and suffix.</p>
                         </td>
                         </td>
                         <td align=center width=600>
                         <td align=center width=600>
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                             <img src="http://2010.igem.org/wiki/images/f/ff/Davidson-MissouriWoptimoose.png" alt="Tools" width=280/>
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                             <a href="http://gcat.davidson.edu/igem10/index.html"><img src="http://2010.igem.org/wiki/images/3/33/Davidson-MoWOligator.png" width=400 height=305 alt="gator"/></a>
                         </td>
                         </td>
                     </tr>
                     </tr>
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         </div>
         </div>
         <br><br>
         <br><br>
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         <div id="simulation">
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         <div id="Optimoose">
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             <center>
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             <a href="http://gcat.davidson.edu/igem10/opt/opt_index.html">
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                <a href=""></a>
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                 <h3>Optimus</h3>
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            </center>
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             </a><a NAME="optimoose">
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            <a NAME="Simulator">
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            <a href="">
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                 <h3>Construct Simulation</h3>
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             </a>
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             <table cellpadding=0 cellspacing=0>
             <table cellpadding=0 cellspacing=0>
                 <tbody>
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                         </td>
                         </td>
                         <td width=580>
                         <td width=580>
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                             <p> In order to be able to better understand how to build our constructs to give us the best system for solving the knapsack problem we created a simulation of the cre-lox system. This simulation allows the user to test several pre-determined constructs that out team came up with.  In addition, one can create their own custom construct from any number of promoters, lox sites, fluorescent proteins, essential genes, and/or terminatorsThe program will then show either a single, animated simulation that will allow the user to see how the lox sites interact, or it will run many simulations and then create a histogram that shows the distribution of what fluorescent proteins were expressed. In addition, if the user chooses to include weights for the "items" and a capacity for the knapsack the program will tell you whether or not your construct has exceeded the capacity. In order to run the program, <a href="http://gcat.davidson.edu/igem10/opt/opt_index.html">download the jar file</a> .  To see the actual percentages behind the histogram, the program needs to be run through a command line tool, but other than that simply double clicking the jar file to run it should be sufficient.</p>
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                             <p>Optimus is a tool designed to allow the user to evaluate the expression level of a gene sequence in <i>E. coli</i> using either RCBS-PC or the CAI formulaAdditionally, the user has the option to optimize or deoptimize the sequence by using one of those two formulas. If the optimized or deoptimized option is selected, the user will be given a new sequence whose codons have been changed to reflect the best, or worst, codons as determined by the selected formula. Assuming the entered sequence is in frame, the amino acid sequence is preserved.
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Optimus serves as a mechanism by which we can engineer optimized and deoptimized sequences which represent a given "weight" to the items in a knapsack based on their protein production.</p>
                         </td>
                         </td>
                         <td align=center width=600>
                         <td align=center width=600>
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                             <img src="http://2010.igem.org/wiki/images/f/fe/Davidson-MissouriWsteph.png" alt="sim" width=355/>
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                             <a href="http://gcat.davidson.edu/igem10/opt/opt_index.html"><img src="http://2010.igem.org/wiki/images/2/25/Davidson-MissouriWoptimus.png" alt="Tools" width=280/></a>
                         </td>
                         </td>
                     </tr>
                     </tr>
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         </div>
         </div>
         <br><br>
         <br><br>
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         <div id="Veripart">
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         <div id="simulation">
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             <center>
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             <a NAME="Simulator">
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                <a href="http://72.22.219.205/sequence"></a>
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            <a href="http://2010.igem.org/wiki/images/4/4a/ConstructSim.zip">
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            </center>
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                 <h3>SimuLox</h3>
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            <a href="http://72.22.219.205/sequence">
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             </a>
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                 <h3>VeriPart</h3>
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             </a><a NAME="veripart">
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             <table cellpadding=0 cellspacing=0>
             <table cellpadding=0 cellspacing=0>
                 <tbody>
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                         </td>
                         </td>
                         <td width=580>
                         <td width=580>
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                             <p>Verifying BioBrick parts can be difficult and tedious by hand.  To solve this problem, we have developed a tool to identify BioBrick parts and devices in a given DNA sequenceWhen a sequence is entered, the program checks the last archived version of the BioBrick Parts RegistryIf your desired part is not found, you can attempt to align the sequence you entered with the part you desireSince this search uses a real-time version of the registry, it can potentially find additional parts. When a device is found in your sequence, you can click to view the sequences of individual parts.</p>  
+
                             <p> In order to better understand our fluorescent protein - TetA constructs, we designed a simulation of the cre-lox system.  This simulation allows the user to test our pre-determined constructs as well as custom constructs from any number of promoters, lox sites, fluorescent proteins, essential genes, and/or terminatorsThe program will then show either a single, animated simulation that will allow the user to see how the lox sites interact, or it will run many simulations and then generate a histogram that shows the distribution of what fluorescent proteins were expressedIn addition, if the user chooses to include weights for the "items" and a capacity for the knapsack the program will tell you whether or not your construct has exceeded the capacityIn order to run the program, <a href="http://2010.igem.org/wiki/images/4/4a/ConstructSim.zip">download the jar file</a>.  To see the actual percentages behind the histogram, the program needs to be run through a command line tool, but other than that simply double clicking the jar file to run it should be sufficient.</p>
                         </td>
                         </td>
                         <td align=center width=600>
                         <td align=center width=600>
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                             <img src="http://2010.igem.org/wiki/images/3/32/Davidson-MissouriWveripart.png" alt="Veri" width=400/>
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                             <a href="http://2010.igem.org/wiki/images/4/4a/ConstructSim.zip"><img src="http://2010.igem.org/wiki/images/8/87/Davidson-MissouriWconsim.png" alt="sim" width=355/></a>
                         </td>
                         </td>
                     </tr>
                     </tr>
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         </div>
         </div>
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        <div id="game">
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         <center><a href="#main_wrapper">Top</a></center>
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            <center>
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                <a href="http://72.22.219.205/knapsack"></a>
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            </center>
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            <a href="http://72.22.219.205/knapsack">
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                <h3>Knapsack Game</h3>
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            </a><A NAME="Knapsack">
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                        <td width=580>
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                            <p>In order to aid in the understanding of the Knapsack Problem, we created a game.  The game has two modes: tutorial and challenge, each of which asks the player to fill a knapsack of a certain capacity with given weighted items.  The tutorial gives tips about the type of problem and only uses six weighted items.  The challenge offers no help and asks the player to choose from nine weighted items. </p>
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                        <td align=center width=600>
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                            <img src="http://2010.igem.org/wiki/images/d/da/Davidson-MissouriWknapsack.png" alt="" width=300/>
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         <center><a href="#menu">Top</a></center>
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Latest revision as of 22:19, 22 October 2010

iGEM Davidson – Missouri Western 2010: Tools

The team has designed several tools to guide and support our iGEM project.













SimuLox

In order to better understand our fluorescent protein - TetA constructs, we designed a simulation of the cre-lox system. This simulation allows the user to test our pre-determined constructs as well as custom constructs from any number of promoters, lox sites, fluorescent proteins, essential genes, and/or terminators. The program will then show either a single, animated simulation that will allow the user to see how the lox sites interact, or it will run many simulations and then generate a histogram that shows the distribution of what fluorescent proteins were expressed. In addition, if the user chooses to include weights for the "items" and a capacity for the knapsack the program will tell you whether or not your construct has exceeded the capacity. In order to run the program, download the jar file. To see the actual percentages behind the histogram, the program needs to be run through a command line tool, but other than that simply double clicking the jar file to run it should be sufficient.

sim


Top