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Team:Davidson-MissouriW/Notebook
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Revision as of 14:29, 27 July 2010 by Dwagnermwsu (Talk | contribs)
Notebook
May 31, 2010
- Tested to find lethal concentration of Tet in plated and liquid mediums
June 2, 2010
- Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP
June 3, 2010
- Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP
June 4, 2010
- Mini prepped RBS-TetA-TT
- RX Fragment digestion for RBS-TetA-TT
June 7, 2010
- Ligate and plasmid check for pSB1A2 or pSB1AK3
June 8, 2010
- Double digest on pBAD-RBS-RFP to make an RS fragment insert.
June 9, 2010
- EcoRI and PstI digest on pLac and pBad colonies.
- Arabinose and IPTG concentration experiments started.
June 10, 2010
- Picked 14 colonies from pLac-RBS-RFP and pLac-RBS-RFP-RBS plates.
- Started inducer curve experiments.
June 11, 2010
- Ligated S03736 into pSB1A7 vector.
- EcoRI/PstI digest on pLac-RBS-RFP-RBS colonies.
June 14, 2010
- Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP
June 3, 2010
- Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP
June 3, 2010
- Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP
June 3, 2010
- Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP
June 3, 2010
- Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP
June 3, 2010
- Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP
June 3, 2010
- Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP
June 3, 2010
- Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP
June 3, 2010
- Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP
June 3, 2010
- Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP
June 3, 2010
- Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP
June 3, 2010
- Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP
June 3, 2010
- Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP
June 3, 2010
- Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP
June 3, 2010
- Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP
June 3, 2010
- Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP
June 3, 2010
- Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP
June 3, 2010
- Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP
June 3, 2010
- Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP
June 3, 2010
- Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP
June 3, 2010
- Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP
June 3, 2010
- Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP
June 3, 2010
- Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP
June 3, 2010
- Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP
June 3, 2010
- Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP
June 3, 2010
- Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP
June 3, 2010
- Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP
June 3, 2010
- Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP
