Team:Davidson-MissouriW/Notebook

From 2010.igem.org

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(Notebook)
(Notebook)
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'''June 3, 2010'''
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'''June 23, 2010'''
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<li>Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP </li>
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<li>Preparative NheI/BamHI digest performed on TetA.
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<li>Preparative EcoRI/NheI digest performed on TetA. </li>
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'''June 3, 2010'''
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'''June 24, 2010'''
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<ul>
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<li>Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP </li>
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<li> Annealed optimized and deoptimized segment 1 TetA ligated into vector.
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<li>Annealed optimized and deoptimized segment 2 TetA ligatied into vector.</li>
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'''June 27, 2010'''
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<hr>
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<ul>
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<li>Inducer curve experiment started on pLac and pLacI constructs.
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<li>Picked colonies from TetA segment 1 & 2 optimized & deoptimized plates. </li>
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Revision as of 14:38, 27 July 2010

Notebook

May 31, 2010


  • Tested to find lethal concentration of Tet in plated and liquid mediums


June 2, 2010


  • Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP


June 3, 2010


  • Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP


June 4, 2010


  • Mini prepped RBS-TetA-TT
  • RX Fragment digestion for RBS-TetA-TT


June 7, 2010


  • Ligate and plasmid check for pSB1A2 or pSB1AK3


June 8, 2010


  • Double digest on pBAD-RBS-RFP to make an RS fragment insert.


June 9, 2010


  • EcoRI and PstI digest on pLac and pBad colonies.
  • Arabinose and IPTG concentration experiments started.


June 10, 2010


  • Picked 14 colonies from pLac-RBS-RFP and pLac-RBS-RFP-RBS plates.
  • Started inducer curve experiments.


June 11, 2010


  • Ligated S03736 into pSB1A7 vector.
  • EcoRI/PstI digest on pLac-RBS-RFP-RBS colonies.


June 14, 2010


  • EcoRI/PstI diagnostic digest performed on pBad-RBS-RFP colonies.
  • Inducer curve experiment started on pBad and pLac constructs.


June 15, 2010


  • Ligated pLac-RBS-RFP + RBS-TetA.
  • IPTG experiment started on pLacI-RBS-RFP


June 16, 2010


  • EcoRI/PstI diagnostic digest performed on pLac-RBS-RFP in pSB1A7 vector.
  • IPTG experiment started on plates with pLac and pLacI constructs.


June 17, 2010


  • Flourimeter data collected from IPTG experiment.
  • Ligated pLacI-RBS-RFP + RBS-TetA.


June 18, 2010


  • Picked 4 colonies from pLacI-RBS-RFP-RBS-TetA plates.
  • IPTG experiment started using newly created IPTG.


June 21, 2010


  • Digested TetA with EcoRI/NheI.
  • Annealed oligos for optimized and deoptimized TetA segment 1.


June 22, 2010


  • Ligated annealed TetA oligos into digested TetA.
  • Inducer curve experiment started on pLac and pLacI constructs.


June 23, 2010


  • Preparative NheI/BamHI digest performed on TetA.
  • Preparative EcoRI/NheI digest performed on TetA.


June 24, 2010


  • Annealed optimized and deoptimized segment 1 TetA ligated into vector.
  • Annealed optimized and deoptimized segment 2 TetA ligatied into vector.


June 27, 2010


  • Inducer curve experiment started on pLac and pLacI constructs.
  • Picked colonies from TetA segment 1 & 2 optimized & deoptimized plates.


June 3, 2010


  • Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP


June 3, 2010


  • Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP


June 3, 2010


  • Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP


June 3, 2010


  • Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP


June 3, 2010


  • Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP


June 3, 2010


  • Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP


June 3, 2010


  • Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP


June 3, 2010


  • Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP


June 3, 2010


  • Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP


June 3, 2010


  • Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP


June 3, 2010


  • Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP


June 3, 2010


  • Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP


June 3, 2010


  • Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP


June 3, 2010


  • Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP


June 3, 2010


  • Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP


June 3, 2010


  • Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP


June 3, 2010


  • Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP


June 3, 2010


  • Mini prepped, diagnostic RP digest, preparative digest, ligation, pBAD+RBS-RFP