Team:Davidson-MissouriW

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     <div id="greenBox"><h3>Characterizing Cre/Lox</h3><br>
     <div id="greenBox"><h3>Characterizing Cre/Lox</h3><br>
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     <p>Randomly choosing objects to place in the knapsack using Cre/Lox recombination</p><br>
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     <p>Foundational Advances were made as 11 novel lox sites for Cre recombination were built for randomly choosing Knapsack objects
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     <a href="http://2010.igem.org/Team:Davidson-MissouriW/CreLox">Details</a>
     <a href="http://2010.igem.org/Team:Davidson-MissouriW/CreLox">Details</a>
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Revision as of 18:45, 28 July 2010

Optimizing Codons


Building weighted items for the Knapsack through codon variation of the TetA gene led to Foundational Advances


Details

Characterizing Cre/Lox


Foundational Advances were made as 11 novel lox sites for Cre recombination were built for randomly choosing Knapsack objects


Details

Measuring Gene Expression


Visualizing the knapsack problem through controlling environmental variables and manipulating gene order


Details

iGEM Davidson – Missouri Western 2010:
Foundational Advances in Biology and the Knapsack Problem

The Davidson/Missouri Western multidisciplinary team is using synthetic biology to address a mathematical problem in Escherichia coli. Specifically, we are addressing the Knapsack Problem, an NP-complete problem that asks, “Given a finite number of weighted items, can one find a subset of these items that completely fills a knapsack of fixed capacity?”

In our design, weighted items are represented by versions of TetA genes that confer measurably distinct levels of tetracycline resistance. We have altered the codons of the wild type TetA gene, optimizing and de-optimizing several segments of the coding sequence. Each TetA variant is coupled with a distinctive fluorescent gene, and each pair of genes is flanked by lox sites. In the presence of Cre protein, the lox mechanism either inverts or excises the coding sequence, yielding different combinations of expressed TetA variants. An expressed variant corresponds to an item being placed in the knapsack. Over-expression of TetA results in cell death, which represents exceeding the capacity of the knapsack. Under-expression of TetA causes the cells to stop growing due to tetracycline in the growth medium, which represents not completely filling the knapsack. Surviving cells correspond to cells within a certain range of TetA production and the fluorescence tag allows for comparative measurement within this range.

The team is also working to develop software tools relevant to the specific project and applicable to projects in the wider synthetic biology community.


Team

Team

View the Davidson-Missouri Westernteampage.

Project

Project

View the work done by Davidson and Missouri Western undergrads.

Notebook

Notebook

View the project's progress via the lab Notebook.

Parts

Parts

View the parts created by our team.

Sponsors

Sponsors

Thank you to our sponsors.

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