Team:Davidson-MissouriW

From 2010.igem.org

(Difference between revisions)
Line 39: Line 39:
     <div class="clear">
     <div class="clear">
     </div>
     </div>
-
     <div id="parts_box"> <center><a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2010&group=Davidson-MissouriW"><img src="http://2010.igem.org/wiki/images/e/e8/Parts.jpg" alt="Parts"/></a></center>
+
     <div id="parts_box"> <center><a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2010&group=Davidson-MissouriW"><img src="http://2010.igem.org/wiki/images/2/26/Davidson-MissouriWParts.jpg" alt="Parts"/></a></center>
         <h3>Parts </h3>
         <h3>Parts </h3>
         <p>Our team <a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2010&group=Davidson-MissouriW">parts</a>.</p>  
         <p>Our team <a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2010&group=Davidson-MissouriW">parts</a>.</p>  

Revision as of 18:13, 27 July 2010

Optimizing Codons

Details

Details

Characterizing Cre/Lox

Details

Details

Measuring Gene Expression

Details

Details

iGEM Davidson – MWSU 2010: Knapsack

The Davidson/Missouri Western multidisciplinary team is using synthetic biology to address a mathematical problem in ''Escherichia coli''. Specifically, we are addressing the Knapsack Problem, an NP-complete problem that asks, “Given a finite number of weighted items, can one find a subset of these items that completely fills a knapsack of fixed capacity?”

In our design, weighted items are represented by versions of ''TetA'' genes that confer measurably distinct levels of tetracycline resistance. We have altered the codons of the wild type ''TetA'' gene, optimizing and de-optimizing several segments of the coding sequence. Each ''TetA'' variant is coupled with a distinctive fluorescent gene, and each pair of genes is flanked by ''lox'' sites. In the presence of Cre protein, the ''lox'' mechanism either inverts or excises the coding sequence, yielding different combinations of expressed ''TetA'' variants. An expressed variant corresponds to an item being placed in the knapsack. Over-expression of ''TetA'' results in cell death, which represents exceeding the capacity of the knapsack. Under-expression of ''TetA'' causes the cells to stop growing due to tetracycline in the growth medium, which represents not completely filling the knapsack. Surviving cells correspond to cells within a certain range of ''TetA'' production and the fluorescence tag allows for comparative measurement within this range.

The team is also working to develop software tools relevant to the specific project and applicable to projects in the wider synthetic biology community. Go to Project Abstract

Team

Team

View Team page

Project

Projects

View the work done by Davidson and Missouri Western undergrads.

Notebook

Notebook

View Notebook.

Parts

Parts

Our team parts.

Sponsors

Sponsors

To look at our sponsors go here.