Team:Davidson-MissouriW

From 2010.igem.org

(Difference between revisions)
Line 3: Line 3:
<body id="home" onload="setPageSize()">
<body id="home" onload="setPageSize()">
   <div id="super_main_wrapper">
   <div id="super_main_wrapper">
-
     <div id="orangeBox"><h3>Codon Optimization</h3>
+
     <div id="orangeBox"><h3>Optimizing Codons</h3>
         <p>Details</p>
         <p>Details</p>
-
         <a href="">Details</a>
+
         <a href="http://2010.igem.org/Team:Davidson-MissouriW/OptimizingCodons">Details</a>
     </div>
     </div>
-
   <div id="greenBox"><h3>Cre</h3>
+
   <div id="greenBox"><h3>Characterizing Cre/Lox</h3>
     <p>Details</p>
     <p>Details</p>
-
     <a href="">Details</a>
+
     <a href="http://2010.igem.org/Team:Davidson-MissouriW/CreLox">Details</a>
   </div>
   </div>
-
   <div id="blueBox"><h3>TetA</h3>
+
   <div id="blueBox"><h3>Measuring Gene Expression</h3>
     <p>Details</p>
     <p>Details</p>
-
     <a href="">Details</a>
+
     <a href="http://2010.igem.org/Team:Davidson-MissouriW/MeasuringExpression">Details</a>
   </div>
   </div>
   <div class="clear"></div>
   <div class="clear"></div>

Revision as of 13:31, 27 July 2010

Optimizing Codons

Details

Details

Characterizing Cre/Lox

Details

Details

Measuring Gene Expression

Details

Details

iGEM Davidson – MWSU 2010: Knapsack

The Davidson/Missouri Western multidisciplinary team is using synthetic biology to address a mathematical problem in ''Escherichia coli''. Specifically, we are addressing the Knapsack Problem, an NP-complete problem that asks, “Given a finite number of weighted items, can one find a subset of these items that completely fills a knapsack of fixed capacity?”

In our design, weighted items are represented by versions of ''TetA'' genes that confer measurably distinct levels of tetracycline resistance. We have altered the codons of the wild type ''TetA'' gene, optimizing and de-optimizing several segments of the coding sequence. Each ''TetA'' variant is coupled with a distinctive fluorescent gene, and each pair of genes is flanked by ''lox'' sites. In the presence of Cre protein, the ''lox'' mechanism either inverts or excises the coding sequence, yielding different combinations of expressed ''TetA'' variants. An expressed variant corresponds to an item being placed in the knapsack. Over-expression of ''TetA'' results in cell death, which represents exceeding the capacity of the knapsack. Under-expression of ''TetA'' causes the cells to stop growing due to tetracycline in the growth medium, which represents not completely filling the knapsack. Surviving cells correspond to cells within a certain range of ''TetA'' production and the fluorescence tag allows for comparative measurement within this range.

The team is also working to develop software tools relevant to the specific project and applicable to projects in the wider synthetic biology community. Go to Project Abstract

Team

Team

View Team page

Tools

Tools

A repository of Tools that can be used....

Notebook

Notebook

View Notebook.

Parts

Parts

Our team parts.

Miscellaneous

For other project details go here






You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.

Project Description

The Davidson/Missouri Western multidisciplinary team is using synthetic biology to address a mathematical problem in Escherichia coli. Specifically, we are addressing the Knapsack Problem, an NP-complete problem that asks, “Given a finite number of weighted items, can one find a subset of these items that completely fills a knapsack of fixed capacity?”
In our design, weighted items are represented by versions of TetA genes that confer measurably distinct levels of tetracycline resistance. We have altered the codons of the wild type TetA gene, optimizing and de-optimizing several segments of the coding sequence. Each TetA variant is coupled with a distinctive fluorescent gene, and each pair of genes is flanked by lox sites. In the presence of Cre protein, the lox mechanism either inverts or excises the coding sequence, yielding different combinations of expressed TetA variants. An expressed variant corresponds to an item being placed in the knapsack. Over-expression of TetA results in cell death, which represents exceeding the capacity of the knapsack. Under-expression of TetA causes the cells to stop growing due to tetracycline in the growth medium, which represents not completely filling the knapsack. Surviving cells correspond to cells within a certain range of TetA production and the fluorescence tag allows for comparative measurement within this range.
The team is also working to develop software tools relevant to the specific project and applicable to projects in the wider synthetic biology community.
Davidson/Missouri Western Team


Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety