Team:DTU-Denmark/Switch

From 2010.igem.org

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<p align="center">UNDER CONSTRUCTION</p>
<p align="center">UNDER CONSTRUCTION</p>
<h1>What is a biological switch?</h1>
<h1>What is a biological switch?</h1>
 +
<p align="justify">A biological switch is a system that enables cells to "remember" a state set by transient signals. This is important biologically because in cases such as differentiation of cells during development, gene regulatory systems must hold the state set during development. This can be accomplished by a network of genes that regulate one another through repressor and activator protein that they encode.</p>
 +
<h1>Design of our Bi[o]stable Switch</h1>
 +
<p align="justify">The simplest of such biological switches is one in which each of two repressor proteins represses the synthesis of the other. When both the repressor proteins are allowed to act, one of two stable states will be observed. In one steady state, the expression of repressor "one" is turned on and expression of repressor "two" is turned off. The repression of expression of repressor "two" is maintained by repressor "one", which means that the repressor "one" essentially acts as its own activator by inhibiting the expression of the repressor, repressor "two", that would repress its expression. In the other steady state, expression of repressor "two" is turned on and expression of repressor "one" is turned off. </p>
 +
<p align="justify">In a system where the repressors can be controlled by outside input signals such as inducers or anti-repressor proteins, the system can be forced into its other stable state. This is illustrated in Figure 1.</p>
 +
<p align="justify"><img src="https://static.igem.org/mediawiki/2010/9/9e/DTU_modeling1.png" width="570px"  align="center"> </img></p>
 +
<p align="justify">We looked to nature for inspiration to design such a switch. The regulatory systems of the lambda phage as well as the Gifsy phages. The Gifsy phages are temperate phages found in <i>Salmonella enterica </i> that have an overall gene organisation typical of the lambdoid phage family (<a href="https://2010.igem.org/Team:DTU-Denmark/Regulatory_sytems" target="_blank" >Regulatory Systems</a>).</p>
 +
<h1>Step-wise Engineering of the Switch</h1>
 +
<p align="justify">The step-wise construction of our Bi[o]stable switch is demonstrated here. </p>
 +
<h2>The Basic building blocks</h2>
<p align="justify"><img src="https://static.igem.org/mediawiki/2010/2/24/DTU_step1.png" width="570px"  align="center"> </img></p>
<p align="justify"><img src="https://static.igem.org/mediawiki/2010/2/24/DTU_step1.png" width="570px"  align="center"> </img></p>
<p align="justify"><img src="https://static.igem.org/mediawiki/2010/c/ce/DTU_step2.png" width="570px"  align="center"> </img></p>
<p align="justify"><img src="https://static.igem.org/mediawiki/2010/c/ce/DTU_step2.png" width="570px"  align="center"> </img></p>
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<p align="justify"><img src="https://static.igem.org/mediawiki/2010/b/b4/DTU_step11.png" width="570px"  align="center"> </img></p>
<p align="justify"><img src="https://static.igem.org/mediawiki/2010/b/b4/DTU_step11.png" width="570px"  align="center"> </img></p>
<p align="justify"><img src="https://static.igem.org/mediawiki/2010/9/99/DTU_step12.png" width="570px"  align="center"> </img></p>
<p align="justify"><img src="https://static.igem.org/mediawiki/2010/9/99/DTU_step12.png" width="570px"  align="center"> </img></p>
-
 
-
<p align="justify">A biological switch is a system that enables cells to "remember" a state set by transient signals. This is important biologically because in cases such as differentiation of cells during development, gene regulatory systems must hold the state set during development. This can be accomplished by a network of genes that regulate one another through repressor and activator protein that they encode.</p>
 
-
<h1>Design of our Bi[o]stable Switch</h1>
 
-
<p align="justify">The simplest of such biological switches is one in which each of two repressor proteins represses the synthesis of the other. When both the repressor proteins are allowed to act, one of two stable states will be observed. In one steady state, the expression of repressor "one" is turned on and expression of repressor "two" is turned off. The repression of expression of repressor "two" is maintained by repressor "one", which means that the repressor "one" essentially acts as its own activator by inhibiting the expression of the repressor, repressor "two", that would repress its expression. In the other steady state, expression of repressor "two" is turned on and expression of repressor "one" is turned off. </p>
 
-
<p align="justify">In a system where the repressors can be controlled by outside input signals such as inducers or anti-repressor proteins, the system can be forced into its other stable state. This is illustrated in Figure 1.</p>
 
-
<p align="justify"><img src="https://static.igem.org/mediawiki/2010/9/9e/DTU_modeling1.png" width="570px"  align="center"> </img></p>
 
-
<p align="justify">We looked to nature for inspiration to design such a switch. The regulatory systems of the lambda phage as well as the Gifsy phages. The Gifsy phages are temperate phages found in <i>Salmonella enterica </i> that have an overall gene organisation typical of the lambdoid phage family (<a href="https://2010.igem.org/Team:DTU-Denmark/Regulatory_sytems" target="_blank" >Regulatory Systems</a>).</p>
 
-
<h1>Step-wise Engineering of the Switch</h1>
 
-
<p align="justify">The step-wise construction of our Bi[o]stable switch is demonstrated here. </p>
 
-
<h2>The Basic building blocks</h2>
 
<p align="justify">The divergent promoters from both Gifsy1 and Gifsy2 phages are utilized in our system. The initial Gifsy1 and Gifsy2 constructs are illustrated below:</p>
<p align="justify">The divergent promoters from both Gifsy1 and Gifsy2 phages are utilized in our system. The initial Gifsy1 and Gifsy2 constructs are illustrated below:</p>
<p align="justify"><img src="https://static.igem.org/mediawiki/2010/a/a5/DTU_basics_of_switch1.png" width="570px"  align="center"> </img></p>
<p align="justify"><img src="https://static.igem.org/mediawiki/2010/a/a5/DTU_basics_of_switch1.png" width="570px"  align="center"> </img></p>

Revision as of 10:47, 12 October 2010

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UNDER CONSTRUCTION

What is a biological switch?

A biological switch is a system that enables cells to "remember" a state set by transient signals. This is important biologically because in cases such as differentiation of cells during development, gene regulatory systems must hold the state set during development. This can be accomplished by a network of genes that regulate one another through repressor and activator protein that they encode.

Design of our Bi[o]stable Switch

The simplest of such biological switches is one in which each of two repressor proteins represses the synthesis of the other. When both the repressor proteins are allowed to act, one of two stable states will be observed. In one steady state, the expression of repressor "one" is turned on and expression of repressor "two" is turned off. The repression of expression of repressor "two" is maintained by repressor "one", which means that the repressor "one" essentially acts as its own activator by inhibiting the expression of the repressor, repressor "two", that would repress its expression. In the other steady state, expression of repressor "two" is turned on and expression of repressor "one" is turned off.

In a system where the repressors can be controlled by outside input signals such as inducers or anti-repressor proteins, the system can be forced into its other stable state. This is illustrated in Figure 1.

We looked to nature for inspiration to design such a switch. The regulatory systems of the lambda phage as well as the Gifsy phages. The Gifsy phages are temperate phages found in Salmonella enterica that have an overall gene organisation typical of the lambdoid phage family (Regulatory Systems).

Step-wise Engineering of the Switch

The step-wise construction of our Bi[o]stable switch is demonstrated here.

The Basic building blocks

The divergent promoters from both Gifsy1 and Gifsy2 phages are utilized in our system. The initial Gifsy1 and Gifsy2 constructs are illustrated below:

These constructs are similar to the half switches seen in Figure 1, the difference being the presence of an extra promoter in front of each regulatory region. The "regulation" of this extra promoter will be described below. The Gifsy1 and Gifsy2 constructs are joined to form the basis of our bistable switch:

The step-wise construction of our Bi[o]stable switch is demonstrated here.

The Final Switch

APPLICATIONS
how we designed our switch - selection of parts and parameters - and last presentation of our system.
Applications
Uncertainties and potential problems:
By designing the switch we did not know the exact distance needed from the nut-site to the terminator steam loop for proper function of anti-termination. We have taken the sequence form the natural seting and made it small enough to give sense as a biological building block.
We have tried to utilize the phage regulation to construct a biological switch that can be used in biological engineering.
When considering how to characterize the subparts of our system we looked at the work already done to characterize terminator efficiency. The screening plasmids made by (REFF) endy and XXX and XXX. The work clearly demonstrates the problem by creating a weldefined data sheet system, the data achived in terms of terminator efficiency is not consistent, and shows the complexity of biology.
It has not been possible for us do all the test needed to develop a wel defined switch system. Below is outlined our approach and in the end we suggest other approaches and possibilities for further work, and considerations in relation to this.

Selection of Parts

Requirements before a biological switch functions. On the paper and theoretically.
Components of the switch
We have decided not to use cI, why? The hong kong paper flaws!! (REF) we did not use UV-activation why? To have a stable system we did not what to use cI and UV-regulatory systems as they can impose problems with the genetic stability.

Modeling

(modeling)

Selecting N protein and nut site

In the end, after evaluating what component pair to use we selected λ N-protein and nut-site. Different nut-sites N-protein systems have been identified and investigated (REFFF), the nutsites for λ-phage and p21, p22, are the best described (REFFF) comparison of the antiterminator effect have not been charfully investigated, as emphasis have been on function and interacting parts. we wanted to selected the nutsite with a strong consistent anti-terminator effect. But as this was not well defined continues work was done with the lambda nut side because more articles and knowledge was available, for potential trouble shooting and improvement of the system interaction and dynamic.

What have been described is that the N-nut-site pair have specific function and thus the λ-N-protein was used for continues, construction of the switch.

Fluorescent Proteins

as we departed in the idea from the terminator screening plasmids described in the partsregistry.org (REFFF) we had a primary focus on fluorescence proteins as our reporter systems. Further we wanted to have high quality data, with a high resolution. We descided on the in-house expertice on using a continous microfermentor system that can measure two fluorescence proteins continuously (biolector) and a flow cytometer, also capable of measuring at two different wave length.