Team:DTU-Denmark/Switch

From 2010.igem.org

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<li><a href="https://2010.igem.org/Team:DTU-Denmark/Background">Background</a></li><br>
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<li><a href="https://2010.igem.org/Team:DTU-Denmark/Background">Synthetic Biology</a></li><br>
<li><a href="https://2010.igem.org/Team:DTU-Denmark/Regulatory_sytems">Regulatory Systems</a></li>
<li><a href="https://2010.igem.org/Team:DTU-Denmark/Regulatory_sytems">Regulatory Systems</a></li>
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<li><a href="https://2010.igem.org/Team:DTU-Denmark/Switch#Biological_Switch">Biological Switches</a></li>
<li><a href="https://2010.igem.org/Team:DTU-Denmark/Switch#Biological_Switch">Biological Switches</a></li>
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<li><a href="https://2010.igem.org/Team:DTU-Denmark/Switch#Bistable_Switches">Bistable Switches</a></li>
<li><a href="https://2010.igem.org/Team:DTU-Denmark/Switch#Design">Design of our Bi[o]stable Switch</a></li>
<li><a href="https://2010.igem.org/Team:DTU-Denmark/Switch#Design">Design of our Bi[o]stable Switch</a></li>
<li><a href="https://2010.igem.org/Team:DTU-Denmark/Switch#Engineering">Step-wise Engineering of the Switch</a></li>
<li><a href="https://2010.igem.org/Team:DTU-Denmark/Switch#Engineering">Step-wise Engineering of the Switch</a></li>
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is triggered in response to fluctuations of a stimulus above or below threshold level, which is
is triggered in response to fluctuations of a stimulus above or below threshold level, which is
frequently mediated by a regulatory circuit with positive and/or negative feedback mechanisms.</p>
frequently mediated by a regulatory circuit with positive and/or negative feedback mechanisms.</p>
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<p align="justify">Some biological switches enable cells to “ remember” a state set by transient signals. Temperate
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<p align="justify">Some biological switches enable cells to “remember” a state set by transient signals. Temperate
bacteriophages are classic examples of such natural genetic switches as they can choose between
bacteriophages are classic examples of such natural genetic switches as they can choose between
two distinct life cycles, namely lytic and lysogenic. For more information please click <a href="https://2010.igem.org/Team:DTU-Denmark/Regulatory_sytems#lambda" target="_blank">here</a>.</p>
two distinct life cycles, namely lytic and lysogenic. For more information please click <a href="https://2010.igem.org/Team:DTU-Denmark/Regulatory_sytems#lambda" target="_blank">here</a>.</p>
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<a href="Bistable Switches"></a><h1>Bistable Switches</h1>
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<a name="Bistable_Switches"></a><h1>Bistable Switches</h1>
<p align="justify">Now, taking an idea from the world of electronics, we define a bistable switch with a memory
<p align="justify">Now, taking an idea from the world of electronics, we define a bistable switch with a memory
function and with two mutually exclusive steady states. Once stability in one state is reached; only
function and with two mutually exclusive steady states. Once stability in one state is reached; only

Revision as of 14:43, 24 October 2010

Welcome to the DTU iGEM wiki!


UNDER CONSTRUCTION

Biological switches

Many biological processes exist in mutually exclusive states. Cells or organisms exist in one of the distinct states depending on the level of stimulus input. A switch from one state to the other is triggered in response to fluctuations of a stimulus above or below threshold level, which is frequently mediated by a regulatory circuit with positive and/or negative feedback mechanisms.

Some biological switches enable cells to “remember” a state set by transient signals. Temperate bacteriophages are classic examples of such natural genetic switches as they can choose between two distinct life cycles, namely lytic and lysogenic. For more information please click here.

Bistable Switches

Now, taking an idea from the world of electronics, we define a bistable switch with a memory function and with two mutually exclusive steady states. Once stability in one state is reached; only stimulus above threshold level would switch the system towards reaching the other steady state. In bistable switches the threshold level of the stimulus input in forward reaction is different from the one in the reverse direction. Such behaviour is known as hysteresis.

Now, taking an idea from the world of electronics, we define a bistable switch with a memory function and with two mutually exclusive steady states. Once stability in one state is reached; only stimulus above threshold level would switch the system towards reaching the other steady state. In bistable switches the threshold level of the stimulus input in forward reaction is different from the one in the reverse direction. Such behaviour is known as hysteresis.

Design of our Bi[o]stable Switch

The simplest of such biological switches is one in which each of two repressor proteins represses the synthesis of the other. When both the repressor proteins are allowed to act, one of two stable states will be observed. In one steady state, the expression of repressor "one" is turned on and expression of repressor "two" is turned off. The repression of expression of repressor "two" is maintained by repressor "one", which means that the repressor "one" essentially acts as its own activator by inhibiting the expression of the repressor, repressor "two", that would repress its expression. In the other steady state, expression of repressor "two" is turned on and expression of repressor "one" is turned off.

In a system where the repressors can be controlled by outside input signals such as inducers or anti-repressor proteins, the system can be forced into its other stable state. This is illustrated in Figure 2.

Figure 2: Simple bistable switch.


We looked to nature for inspiration to design such a switch. The regulatory systems of the lambda phage as well as the Gifsy phages. The Gifsy phages are temperate phages found in Salmonella enterica that have an overall gene organisation typical of the lambdoid phage family (for more theory please see Regulatory Systems).

Step-wise Engineering of the Switch

The step-wise construction of our Bi[o]stable switch is demonstrated here, parts will be added to the switch as we build it up:

Step 1

The divergent promoters from both Gifsy1 and Gifsy2 phages are utilized in our system. The initial Gifsy1 and Gifsy2 constructs are illustrated below, Figure 3 and Figure 4, respectively.

Figure 3: The divergent promoters from the Gifsy 1 and Gifsy 2 phages have been highlighted. The divergent promoters from Gifsy 1 are pR1 and pRM1, whereas the Gifsy 2 divergent promoters are pR2 and pRM2.


Figure 4: Both sets of divergent promoters have been highlighted. As illustrated, GogR (Gifsy 1 phage repressor) will repress the pR1 promoter when it is expressed. Transcription of gtgR is still allowed to some degree due to the fact that GtgR does not also repress the pRM2 promoter.
Note:The strategy for the leakiness of pRM2 will be introduced later in Steps 3 and 4.


Figure 5: As similarly demonstrated in Figure 4, both sets of divergent promoters have been highlighted. GtgR (Gifsy 2 phage repressor) will repress the pR2 promoter when it is expressed. Transcription of gogR is still allowed to some degree due to fact that GtgR does not also repress the pRM1 promoter.
Note:The strategy for the leakiness of pRM1 will be introduced later in Steps 3 and 4.


Step 2

Figure 6: antO and antT are introduced in this image. antO codes for the anti-repressor protein responsible for preventing GogR from binding to and thereby repressing the pR1 promoter. antT codes for the anti-repressor protein responsible for preventing GtgR from binding to and thereby repressing the pR2 promoter.


Figure 7: The action of the anti-repressor protein, AntT from the Gifsy 2 phage is illustrated. AntT binds to repressor protein GtgR thereby preventing its repressor action. This means that GtgR is no longer able to bind to the pR2 promoter leaving the expressed GogR to bind to the pR1 promoter.
Note: The strategy for the leakiness of the pRM2 promoter will be introduced in Steps 3 and 4.


Figure 8: The action of the anti-repressor protein, AntO from the Gifsy 1 phage is illustrated. AntO binds to repressor protein GogR thereby preventing its repressor action. This means that GogR is no longer able to bind to the pR1 promoter leaving the expressed GtgR to bind to the pR2 promoter.
Note: The strategy for the leakiness of the pRM1 promoter will be introduced in Steps 3 and 4.


Step 3

Figure 9: The nut sites from p22 and lambda phages are introduced into the construct (for theory see Regulatory Systems). These nut sites will contribute to the robustness of the switch as described in Step 4.


Step 4

Figure 10: The anti-terminator proteins, Gp24 and GpN are introduced. In this image, Gp24 has been expressed and by binding to the p22 nut site, it enables RNA polymerase to bypass the terminator and continue transcription. This contributes to the robustness of the bistable switch as even though the pRM1 promoter allow transcription through them. Transcription through the terminator is prevented without the presence of the anti-terminator thereby not allowing spontaneous change of states.


Figure 11: In this image, GpN has been expressed and by binding to the lambda nut site, it enables RNA polymerase to bypass the terminator and continue transcription. Transcription through the terminator by GpN allows continuous expression of anti-terminator as well as anti-repressor, which will keep the cell in the induced state and thereby red. Even though transcription through pRM1 is permitted and GogR is expressed, it will be derepressed by the anti-repressor thus inactivating GogR. The state is maintained and the cell emits geen light.


In the switch design, each half switch contains a nut site followed by a terminator, as well as an antiterminator. The roles of these parts are to increase the stability of the current state of the switch. The pRM promoters are not very well repressed by the GogR/GtgR repressors and promotes transcription even in their presence. If transcription was allowed to continue to the antirepressor located on the inactive switch, the switch could change state spontaneously. The terminator ensures that this does not happen. The antiterminator of the active state is expressed, allowing continued transcription past the terminator.

Step 5

Now that we are able to keep the switch in each state stably, we also need a means to be able to switch the state of the switch at will. To do this we introduce two input plasmids illustrated in Figure 13. The plasmids contain the antiterminators and antirepressors of each state respectively, in front of two different inducible promoters.

Figure 13: Our final construct is illustrated.


When the plasmid corresponding to state 1 is induced, the switch is forced into state 1. This happens because the state’ s antiterminator and antirepressor are produced. The antirepressor inactivates state 2’ s repressor, resulting in increased transcription through state 1. At the same time, the antiterminator allows transcription to continue past state 1’ s terminator, where the genes for the same antirepressor and antiterminator are located. Thus, with a sufficient amount of induction, the switch will have switched states.

The Final Switch

Figure 13: Our final construct is illustrated.