Team:DTU-Denmark/SPL
From 2010.igem.org
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<li><a href="https://2010.igem.org/Team:DTU-Denmark/Basics">Basics</a></li><br> | <li><a href="https://2010.igem.org/Team:DTU-Denmark/Basics">Basics</a></li><br> | ||
- | <li><a href="https://2010.igem.org/Team:DTU-Denmark/Regulatory_sytems">Regulatory Systems</a></li><br> | + | <li><a href="https://2010.igem.org/Team:DTU-Denmark/Regulatory_sytems">Regulatory Systems</a></li> |
- | <li><a href="https://2010.igem.org/Team:DTU-Denmark/Switch">The Switch</a></li>< | + | <ul><font size="2"> |
- | <li><a href="https://2010.igem.org/Team:DTU-Denmark/SPL">Synthetic Promoter Library</a></li><br> | + | <li><a href="https://2010.igem.org/Team:DTU-Denmark/Regulatory_sytems#lambda">Lambda Phage</a></li> |
- | <li ><a href="https://2010.igem.org/Team:DTU-Denmark/ | + | <li><a href="https://2010.igem.org/Team:DTU-Denmark/Regulatory_sytems#gifsy">Gifsy Phage</a></li> |
+ | </font></ul> | ||
+ | <br> | ||
+ | <li><a href="https://2010.igem.org/Team:DTU-Denmark/Switch">The Switch</a></li> | ||
+ | <ul><font size="2"> | ||
+ | <li><a href="https://2010.igem.org/Team:DTU-Denmark/Switch#Biological_Switch">What is a biological switch?</a></li> | ||
+ | <li><a href="https://2010.igem.org/Team:DTU-Denmark/Switch#Design">Design of our Bi[o]stable Switch</a></li> | ||
+ | <li><a href="https://2010.igem.org/Team:DTU-Denmark/Switch#Engineering">Step-wise Engineering of the Switch</a></li> | ||
+ | <li><a href="https://2010.igem.org/Team:DTU-Denmark/Switch#Applications_of_our_Bi[o]stable_switch">Applications of our Bi[o]stable switch</a></li> | ||
+ | </font></ul> | ||
+ | <br><li><a href="https://2010.igem.org/Team:DTU-Denmark/SPL">Synthetic Promoter Library</a></li><br> | ||
+ | <li ><a href="https://2010.igem.org/Team:DTU-Denmark/Modelling">Modeling</a></li><br> | ||
</ul> | </ul> | ||
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Revision as of 10:37, 24 October 2010
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Synthetic Promoter Library Modulation of gene expression such as of cellular enzyme activities[1] as well as regulation of transcription are amongst some of the areas where Synthetic Promoter Libraries (SPLs) are currently being used. SPL provides an alternative method for gene regulation compared to the old methods, namely those of gene knockout as well as strong over expression, these two usually executed on the basis of apparent rate limiting steps[1].
The spacer sequences that surround the consensus regions contribute significantly to the strengths of promoters[1]. In our design, we decided to both randomize the spacer sequences as well as randomize 2 bases in both of the consensus regions as seen in the provided diagram. N stands for 25% each of A, C, G and T, while R stands for 50% each of A and G, and W stands for 50% A and T. The point of randomizing both would be to obtain a promoter library that is not biased towards being all strong, by giving 2 bases within each of the consensus regions a 50% chance of being their original bases only 1/16 of all promoters will be strong, this being without taking into consideration the fraction of strong promoters obtainable from the randomized spacer sequences. |