Team:DTU-Denmark/Parts

From 2010.igem.org

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<p align="justify">Gifsy1 is a temperate phage present in many of the Salmonella enterica serovar Typhimurium strains. This part contains the Gifsy1 promoters and the Gifsy1 repressor. The promoters are divergent; consisting of pR[Gifsy1] and pRM[Gifsy1]. Downstream of the pRM[Gifsy1] promoter is the repressor gogR. gogR binds to the operator regions located between the two divergent promoters and is predicted to act both as an activator of the pRM[Gifsy1] and a repressor of the pR[Gifsy1]. The regulatory system is similar to the one found in lambda phages but induction is repressor cleavage independent. Instead small lexA regulated anti-repressor proteins bind to the repressor and prevents its DNA activity. The part has been used and tested in <i>E. Coli</i>.</p>
<p align="justify">Gifsy1 is a temperate phage present in many of the Salmonella enterica serovar Typhimurium strains. This part contains the Gifsy1 promoters and the Gifsy1 repressor. The promoters are divergent; consisting of pR[Gifsy1] and pRM[Gifsy1]. Downstream of the pRM[Gifsy1] promoter is the repressor gogR. gogR binds to the operator regions located between the two divergent promoters and is predicted to act both as an activator of the pRM[Gifsy1] and a repressor of the pR[Gifsy1]. The regulatory system is similar to the one found in lambda phages but induction is repressor cleavage independent. Instead small lexA regulated anti-repressor proteins bind to the repressor and prevents its DNA activity. The part has been used and tested in <i>E. Coli</i>.</p>
Reference
Reference
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<p align="justify">BBa_K374009
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<p align="justify">BBa_K374009</p>
<font size="3"><b>Divergent promoters from Gifsy2 phage and Gifsy2 repressor GtgR</b></font><br>
<font size="3"><b>Divergent promoters from Gifsy2 phage and Gifsy2 repressor GtgR</b></font><br>
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<p align="justify"><i>This regulatory biobrick can be used in a biological circuit. Other BioBricks can be inserted downstream of the pR promoter, however, no expression will be allowed because of the GtgR repressor. BBa_K374009 can be combined with XXX hence allowing expression of your inserted BioBrick.</i></p>
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</i></p>
<p align="justify">Gifsy2 is a temperate phage present in many of the Salmonella enterica serovar Typhimurium strains. This part contains the Gifsy2 promoters and the Gifsy2 repressor. The promoters are divergent; consisting of pR[Gifsy2] and pRM[Gifsy2]. Downstream of the pRM[Gifsy2] promoter is the repressor gtgR. gtgR binds to the operator regions located between the two divergent promoters and is predicted to act both as an activator of the pRM[Gifsy2] and a repressor of the pR[Gifsy2]. The regulatory system is similar to the one found in lambda phages but induction is repressor cleavage independent. Instead small lexA regulated anti-repressor proteins bind to the repressor and prevents its DNA activity. The part has been used and tested in <i>E. Coli</i>.</p>
<p align="justify">Gifsy2 is a temperate phage present in many of the Salmonella enterica serovar Typhimurium strains. This part contains the Gifsy2 promoters and the Gifsy2 repressor. The promoters are divergent; consisting of pR[Gifsy2] and pRM[Gifsy2]. Downstream of the pRM[Gifsy2] promoter is the repressor gtgR. gtgR binds to the operator regions located between the two divergent promoters and is predicted to act both as an activator of the pRM[Gifsy2] and a repressor of the pR[Gifsy2]. The regulatory system is similar to the one found in lambda phages but induction is repressor cleavage independent. Instead small lexA regulated anti-repressor proteins bind to the repressor and prevents its DNA activity. The part has been used and tested in <i>E. Coli</i>.</p>
</p>
</p>

Revision as of 09:54, 27 October 2010

Welcome to the DTU iGEM wiki!

BioBricks submitted

BBa_K374008

Divergent promoters from Gifsy1 phage and Gifsy1 repressor GogR

This regulatory biobrick can be used in a biological circuit. Other BioBricks can be inserted downstream of the pR promoter, however, no expression will be allowed because of the GogR repressor. BBa_K374008 can be combined with BBa_K3740010 hence allowing expression of your inserted BioBrick.

Gifsy1 is a temperate phage present in many of the Salmonella enterica serovar Typhimurium strains. This part contains the Gifsy1 promoters and the Gifsy1 repressor. The promoters are divergent; consisting of pR[Gifsy1] and pRM[Gifsy1]. Downstream of the pRM[Gifsy1] promoter is the repressor gogR. gogR binds to the operator regions located between the two divergent promoters and is predicted to act both as an activator of the pRM[Gifsy1] and a repressor of the pR[Gifsy1]. The regulatory system is similar to the one found in lambda phages but induction is repressor cleavage independent. Instead small lexA regulated anti-repressor proteins bind to the repressor and prevents its DNA activity. The part has been used and tested in E. Coli.

Reference

BBa_K374009

Divergent promoters from Gifsy2 phage and Gifsy2 repressor GtgR

This regulatory biobrick can be used in a biological circuit. Other BioBricks can be inserted downstream of the pR promoter, however, no expression will be allowed because of the GtgR repressor. BBa_K374009 can be combined with XXX hence allowing expression of your inserted BioBrick.

Gifsy2 is a temperate phage present in many of the Salmonella enterica serovar Typhimurium strains. This part contains the Gifsy2 promoters and the Gifsy2 repressor. The promoters are divergent; consisting of pR[Gifsy2] and pRM[Gifsy2]. Downstream of the pRM[Gifsy2] promoter is the repressor gtgR. gtgR binds to the operator regions located between the two divergent promoters and is predicted to act both as an activator of the pRM[Gifsy2] and a repressor of the pR[Gifsy2]. The regulatory system is similar to the one found in lambda phages but induction is repressor cleavage independent. Instead small lexA regulated anti-repressor proteins bind to the repressor and prevents its DNA activity. The part has been used and tested in E. Coli.


Parts

New for iGEM 2010 is the groupparts tag. This tag will generate a table with all of the parts that your team adds to your team sandbox. Note that if you want to document a part you need to document it on the Registry, not on your team wiki.

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