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| - | <h2>Introduction</h2>
| + | <p align="center"><img src="https://static.igem.org/mediawiki/2010/e/ef/DTU_BB_Repressor1.png" width="570px" align="center"> </img></p> |
| - | <p align="justify">Mainly the hard control of the switch is due to a double regulation system build on a both terminator-anti-terminator and repressor anti-repressor regulation. It was out of the scope of this project to construct the entire theoretical developed switch, and characterize the fully constructed switch. Have focused on characterizing the two regulatory systems individually. This was done in order to investigate if the responses were satisfactory to use in a future complete switch construction. (???? By getting the regulatory mechanism of the subparts we further, by modeling, could conclude constraints for successful function of the system and other subparts. | + | <p align="center"><img src="https://static.igem.org/mediawiki/2010/7/7c/DTU_BB_Repressor2.png" width="570px" align="center"> </img></p> |
| - | In this section we describe the design of and the experimental setup used to characterize the subparts of the system and our bio-bricks.</p>
| + | <p align="center"><img src="https://static.igem.org/mediawiki/2010/3/34/DTU_BB_Repressor3_graph.png" width="570px" align="center"> </img></p> |
| - | <h2>Anti-Terminator</h2>
| + | <p align="center"><img src="https://static.igem.org/mediawiki/2010/f/ff/DTU_Project_illustration_1.png" width="570px" align="center"> </img></p> |
| - | <p align="justify">Introduction to this part</p>
| + | <p align="center"><img src="https://static.igem.org/mediawiki/2010/a/a0/DTU_BB_AntiT1.png" width="570px" align="center"> </img></p> |
| - | <h3>Selecting subparts</h3>
| + | <p align="center"><img src="https://static.igem.org/mediawiki/2010/7/7b/DTU_BB_AntiT2.png" width="570px" align="center"> </img></p> |
| - | <p align="justify">Why were these subparts chosen ?<br> | + | <p align="center"><img src="https://static.igem.org/mediawiki/2010/8/84/DTU_BB_AntiT3_graph.png" width="570px" align="center"> </img></p> |
| - | AIM</p>
| + | |
| - | <h3>Design and experimental setup</h3>
| + | |
| - | <p align="justify">presentation - Figure of setup and explanation</p>
| + | |
| - | <h3>Materials and methods</h3>
| + | |
| - | <p align="justify">HOW ? what plasmids and why, what measurering method and why?<br> | + | |
| - | refer to the notebook page with protocols - and actual info from lab.<br>
| + | |
| - | <b>mRNA-stability</b><br>
| + | |
| - | when introducing non-coding sequences problems will acour with rna-degredation of RNAP is not attracted to the area, to fast degredation, unwanted steam loops. (Reference to the terminator screening plasmids for BB)<br>
| + | |
| - | <h3>Results</h3> | + | |
| - | <p align="justify">comments to the results and reference to the BB pages with info and results.</p><br> | + | |
| - | | + | |
| - | <h2>Repressor function</h2>
| + | |
| - | <p align="justify">Introduction to this part</p>
| + | |
| - | <h3>Selecting subparts</h3>
| + | |
| - | <p align="justify">Why were these subparts chosen ?<br> | + | |
| - | AIM</p>
| + | |
| - | <h3>Design and experimental setup</h3>
| + | |
| - | <p align="justify">presentation - Figure of setup and explanation</p>
| + | |
| - | <h3>Materials and methods</h3>
| + | |
| - | <p align="justify">HOW ? what plasmids and why, what measurering method and why?<br> | + | |
| - | refer to the notebook page with protocols - and actual info from lab.<br>
| + | |
| - | <h3>Results</h3> | + | |
| - | <p align="justify">comments to the results and reference to the BB pages with info and results.</p><br> | + | |
| - | | + | |
| - | <h2>Synthetic promoter library (SPL)</h2>
| + | |
| - | How does it work, examples, what have it been used to characterize?<br>
| + | |
| - | how do you construct it? Figures and illustrations to explain.<br>
| + | |
| - | Figures to explain our use? And example on our specific design primer sequences illustration on the double stranded DNA, with BB - prefix suffix.<br></p>
| + | |
| | </td> | | </td> |
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