Team:DTU-Denmark

From 2010.igem.org

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Revision as of 08:04, 5 October 2010

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Welcome to the DTU iGEM wiki!


Sponsors and acknowledgements

- List of sponsors
- Acknowledgements


Also check out our Danish website

- DTU iGEM 2010


Also check us out on:

- Facebook
- Twitter


Our Galllery:

Picture gallery!

DTU DENMARK

The aim of this project is to engineer a genetic bi-stable switch that produces two different, mutually exclusive outputs when given two different inputs. The switch is based on the repressor-anti-repressor system of the salmonella phages Gifsy1 and Gifsy2 and the λ-phage anti-termination system. The latest induced output will remain stable through generations, even once the input ceases, due to the phage regulatory systems.

We present the framework for this development and characterize the regulatory mechanisms by using fluorescent proteins as the reporter (outputs). The dynamics of the system have been modeled and we have also attempted to characterize and submit the promoters, repressors and anti-repressors from the salmonella phages, as well as the two anti-terminator proteins from the lambda phage, as BioBricks.

Basic Concept

Figure 1


Figure 1 shows the basic concept of our project:
Inducer 1 will activate Promoter 1 thereby start up the production of Repressor 1, switching off the other part of our switch by repressing Promoter 2. This will essentially make the cells change from red to green!

This means that when the cells are exposed to Inducer 2, the cell culture will turn from green to red.


Quick Update

October 1st, 2010
Check out our active blog: now with picture of the day!

August 14th, 2010
Pictures have been posted so check out our picture gallery!

Comments or questions to the team? Please