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| - | <a href="https://2010.igem.org/Team:Calgary/Project">Overview</a>
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| - |
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| - | <div class="twitterTicker">
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| - | <p><a href="http://twitter.com/iGEMCalgary">Follow us on Twitter!</a></p>
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| - | <div class="boxContainer">
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| |
| - | <img src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Modellingtxt.png"></img>
| |
| - | <p>Our modelling project description will arrive soon! Keep checking back!</p>
| |
| - | </div>
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| - |
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| - | <div class="boxSpacer"></div>
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| - | <img src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Circuit1.png"></img>
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| - | <p>Description of Circuit 1 goes here.</p>
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| - | </div>
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| - | <img src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Circuit2.png"></img>
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| - | <div class="projectDescContainer">
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| - | <img class="descTitle" title="Project Description"
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| - |
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| - | src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/ProjectDescription.png"></img>
| |
| - | <p>Many synthetic biology projects involve the expression of recombinant proteins in microorganisms such as E. coli. The problems
| |
| - |
| |
| - | encountered with many synthetic biology projects often involve problems with protein expression. It is often very difficult to recognize the
| |
| - |
| |
| - | problem and pinpoint where it lies. The goal of the University of Calgary 2010 iGEM team is to build a protein expression "troubleshooting kit".
| |
| - |
| |
| - | This kit will contain two systems with which target genes can be inserted. In the resulting cell growth, fluorescent protein production will be
| |
| - |
| |
| - | used to determine whether there is a problem with protein expression as well as indicate where the protein expression is failing.</p>
| |
| - | <p>Protein expression happens in three steps: the transcription of the DNA to mRNA, the translation of mRNA into an amino acid
| |
| - |
| |
| - | sequence, and the folding of that amino acid sequence into a protein. Our system detects possible errors in these three steps using two circuits.
| |
| - |
| |
| - | The first circuit has a fluorescent reporter that is produced when DNA is transcribed into mRNA and another that is produced when mRNA is
| |
| - |
| |
| - | translated into a functional protein. When both reporter proteins are expressed in the cell, it indicates both transcription and translation are
| |
| - |
| |
| - | successful. The second circuit involves reporter systems that are activated as a result of protein misfolding. Two native stress-activated
| |
| - |
| |
| - | promoters from E. coli were engineered upstream to fluorescent reporters that will respond to periplasmic and cytoplasmic protein misfolding. If
| |
| - |
| |
| - | the protein of interest misfolds in either area of the cell, one of the promoters will be activated and the corresponding fluorescence will be
| |
| - |
| |
| - | observed.</p>
| |
| - | <p><a href="https://2010.igem.org/Team:Calgary/Project">Read more...</a></p>
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| - | <table>
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| - | <td><a href="#"><img src="http://www.sciencemediacentre.ca/smc/images/stories/logos/AI%
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