Team:Chiba/aa

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<img src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Modellingtxt.png"></img>
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<p>Our modelling project description will arrive soon! Keep checking back!</p>
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<p>Many synthetic biology projects involve the expression of recombinant proteins in microorganisms such as E. coli. The problems
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encountered with many synthetic biology projects often involve problems with protein expression. It is often very difficult to recognize the
+
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problem and pinpoint where it lies. The goal of the University of Calgary 2010 iGEM team is to build a protein expression "troubleshooting kit".
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This kit will contain two systems with which target genes can be inserted. In the resulting cell growth, fluorescent protein production will be
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used to determine whether there is a problem with protein expression as well as indicate where the protein expression is failing.</p>
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<p>Protein expression happens in three steps: the transcription of the DNA to mRNA, the translation of mRNA into an amino acid
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sequence, and the folding of that amino acid sequence into a protein. Our system detects possible errors in these three steps using two circuits.
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The first circuit has a fluorescent reporter that is produced when DNA is transcribed into mRNA and another that is produced when mRNA is
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translated into a functional protein. When both reporter proteins are expressed in the cell, it indicates both transcription and translation are
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successful. The second circuit involves reporter systems that are activated as a result of protein misfolding. Two native stress-activated
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promoters from E. coli were engineered upstream to fluorescent reporters that will respond to periplasmic and cytoplasmic protein misfolding. If
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the protein of interest misfolds in either area of the cell, one of the promoters will be activated and the corresponding fluorescence will be
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observed.</p>
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<p><a href="https://2010.igem.org/Team:Calgary/Project">Read more...</a></p>
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Latest revision as of 07:27, 31 August 2010

Retrieved from "http://2010.igem.org/Team:Chiba/aa"