Team:Chiba/System 2/Result

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<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Overall project</span></a></li>
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<li><a href="https://2010.igem.org/Team:Chiba/System_1"><span>System 1</span></a></li>
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<li><a href="https://2010.igem.org/Team:Chiba/System_2"><span>System 2</span></a></li>                        </ul>
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<li><a href="https://2010.igem.org/Team:Chiba/System_2/Testing_individual_parts"><span>Testing</span></a></li>
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<li><a href="https://2010.igem.org/Team:Chiba/System_2/Construction_Process"><span>Construction</span></a></li>
 +
<li><a href="https://2010.igem.org/Team:Chiba/System_2/Result"><span>Result</span></a></li>
 +
<li><a href="https://2010.igem.org/Team:Chiba/System_2/Evaluation_subsystem"><span>Evaluation</span></a></li>
 +
<li><a href="https://2010.igem.org/Team:Chiba/System_2/Remarks"><span>Remarks</span></a></li>
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<!--- 3rd tab End --->
==Abstract==
==Abstract==
BBa_R0061は-35および-10の間にlux box を含む配列である。継続的に下流遺伝子の転写を行う。しかしながら，LuxR-AHL複合体によって下流の遺伝子転写を抑制する。<br>
BBa_R0061は-35および-10の間にlux box を含む配列である。継続的に下流遺伝子の転写を行う。しかしながら，LuxR-AHL複合体によって下流の遺伝子転写を抑制する。<br>

Revision as of 18:56, 27 October 2010




 

 

 

Contents

Abstract

BBa_R0061は-35および-10の間にlux box を含む配列である。継続的に下流遺伝子の転写を行う。しかしながら，LuxR-AHL複合体によって下流の遺伝子転写を抑制する。
私たちは，Plux invの下流にRBSおよびGFP遺伝子を挿入したプラスミド (Plux inv-GFP) を作製し，抑制機能を確認した。
Biobrick number R0061 contains lux box between -35 and -10 and fuctions LuxR-AHL inverter.

We've prepare Plux inv-GFP and characterized about it.

Experiments

1.Preparing Plux inv-GFP

1．Plux inv－GFPの作製 クローニングのprocessをFig. 1に示す。作製したPlux inv-GFPを形質転換した株は緑色蛍光を示した。また，sequenceも正しいことを確認した。 Preparing Plux inv-GFP process is shown in Fig. 1.

Fig. 1 　Evaluation of LuxR Inverter

2.Characterization Plux inv-GFP

Plux inv-GFP およびLuxR generatorである Plac-LuxRをDH10B株に共形質転換した。コロニーをつついてLB液体培地(AHL0および1000 nM)で12 h培養し, スピンダウンしてペレットを観察した。

Fig. 2 assay protocol

Results

Fig. 3

Reference

  1. Egland.K.A, and Greenberg.E.P, Conversion of the Vibrio Fischeri Transcriptional Activator,LuxR, to a Repressor, J. Bacteriol., 182, P.805-811 (2000)
  2. Cox.R.S.3rd, Surette.M.G, Elowitz.M.B, Programming gene expression with combinatorial promoters, Mol Syst Biol, 3 ,145 (2007)
Retrieved from "http://2010.igem.org/Team:Chiba/System_2/Result"