"
Team:Chiba/System 1/Testing components
From 2010.igem.org
(→Explanation of Circuit) |
(→Testing individual parts) |
||
| Line 366: | Line 366: | ||
For Pulse generator, the parts shown below were checked. | For Pulse generator, the parts shown below were checked. | ||
| + | |||
1. Lux/CI434 hybrid promoter<br> | 1. Lux/CI434 hybrid promoter<br> | ||
2. Pulse generator | 2. Pulse generator | ||
Also, we checked if there were no crosstalk between CI/CI434 and CI promoter/CI434 promoter. | Also, we checked if there were no crosstalk between CI/CI434 and CI promoter/CI434 promoter. | ||
Revision as of 20:01, 27 October 2010
Testing individual parts
To realize the genetic double click system, we made two plasmids. First one is “GFP generator”, which generates the output (GFP expression) on condition that T7 RNAP activates the GFP upstream promoter and also CI protein does not repress that promoter. Second one is “Pulse generator”, which generate the pulse of T7 RNAP in response to the 1st and 2nd input. The subparts of those plasmids are shown in Fig.1.
Regarding with those two plasmids, we tested the function of each parts in the plasmids.
For GFP generator, the parts shown below were checked.
1. Constitutive promoter
2. LuxR
3. T7/CI-OR1 hybrid promoter
For Pulse generator, the parts shown below were checked.
1. Lux/CI434 hybrid promoter
2. Pulse generator
Also, we checked if there were no crosstalk between CI/CI434 and CI promoter/CI434 promoter.
