Team:Chiba/Parts/test

From 2010.igem.org

(Difference between revisions)
(Hybrid promoter)
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!align="center"|[[Team:Chiba/Project|The Project]]
!align="center"|[[Team:Chiba/Project|The Project]]
!align="center"|[[Team:Chiba/Parts|Parts Submitted to the Registry]]
!align="center"|[[Team:Chiba/Parts|Parts Submitted to the Registry]]
-
!align="center"|[[Team:Chiba/Reference|Reference]]
+
!align="center"|[[Team:Chiba/Results|Result]]
!align="center"|[[Team:Chiba/Notebook|Notebook]]
!align="center"|[[Team:Chiba/Notebook|Notebook]]
-
!align="center"|[[Team:Chiba/Acknowledgements|Acknowledgements]]
+
!align="center"|[[Team:Chiba/support|support]]
|}
|}
{| align="justify"
{| align="justify"
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!align="center"|[[Team:Chiba/Project|The Project]]
!align="center"|[[Team:Chiba/Project|The Project]]
!align="center"|[[Team:Chiba/Parts|Parts Submitted to the Registry]]
!align="center"|[[Team:Chiba/Parts|Parts Submitted to the Registry]]
-
!align="center"|[[Team:Chiba/Reference|Reference]]
+
!align="center"|[[Team:Chiba/Results|Result]]
!align="center"|[[Team:Chiba/Notebook|Notebook]]
!align="center"|[[Team:Chiba/Notebook|Notebook]]
-
!align="center"|[[Team:Chiba/Acknowledgements|Acknowledgements]]
+
!align="center"|[[Team:Chiba/support|support]]
|}
|}
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===Hybrid promoter===
===Hybrid promoter===
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<td width="200">Sequenced<td>
<td width="200">Sequenced<td>
<td width="200">Sent<td>
<td width="200">Sent<td>
-
<tr>
+
</tr>
<tr>
<tr>
<td width="200">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396002 BBa_K396002]</td>
<td width="200">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K396002 BBa_K396002]</td>
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<td width="200">Sequenced<td>
<td width="200">Sequenced<td>
<td width="200">Sent<td>
<td width="200">Sent<td>
-
 
</tr>
</tr>
<tr>
<tr>
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<td width="50">ok<td>
<td width="50">ok<td>
<td width="50">ok<td>
<td width="50">ok<td>
-
 
</tr>
</tr>
<tr>
<tr>
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<td width="200">2886<td>
<td width="200">2886<td>
<td width="200">Cm<td>
<td width="200">Cm<td>
-
<td width="350">80/miniprepped<td>
+
<td width="350">miniprepped<td>
<td width="50">ok<td>
<td width="50">ok<td>
<td width="50">ok<td>
<td width="50">ok<td>
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<td width="50">OK<td>
<td width="50">OK<td>
</tr>
</tr>
-
 
-
===Receiver protein===
 
-
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000" center>
 
-
<tr>
 
-
<td width="200">Part Name</td>
 
-
<td width="200">Sub Parts</td>
 
-
<td width="200">Part Length(bp)<td>
 
-
<td width="200">Plasmid Vector<td>
 
-
<td width="200">Origin<td>
 
-
<td width="300">Part Length(bp)<td>
 
-
<td width="200">Part and vector length(bp)<td>
 
-
<td width="200">Resistance<td>
 
-
<td width="200">Status<td>
 
-
<td width="200">Sequenced<td>
 
-
<td width="200">Sent<td>
 
-
</tr>
 
-
<tr>
 
-
<td width="200">[http://partsregistry.org/Part:BBa_K084004 BBa_K084004]</td>
 
-
<td width="300">ptet+rbs+RhlR</td>
 
-
<td width="200">867<td>
 
-
<td width="200">pSB1A2<td>
 
-
<td width="200">pMB1<td>
 
-
<td width="200">2079<td>
 
-
<td width="200">2946<td>
 
-
<td width="200">Amp<td>
 
-
<td width="350">miniprepped<td>
 
-
<td width="50">ok<td>
 
-
<td width="50">[[Image:Chiba check.png]]<td>
 
-
</tr>
 
-
<tr>
 
-
<td width="200">[http://partsregistry.org/Part:BBa_K084004 BBa_K084004]</td>
 
-
<td width="300">ptet+rbs+RhlR</td>
 
-
<td width="200">867<td>
 
-
<td width="200">pSB3K3<td>
 
-
<td width="200">p15A<td>
 
-
<td width="200">2750<td>
 
-
<td width="200">3617<td>
 
-
<td width="200">Kan<td>
 
-
<td width="350">miniprepped<td>
 
-
<td width="50">Not yet<td>
 
-
<td width="50">[[Image:Chiba check.png]]<td>
 
-
</tr>
 
-
<tr>
 
-
<td width="200">[http://partsregistry.org/Part:BBa_K084005 BBa_K084005]</td>
 
-
<td width="300">ptet+rbs+LasR</td>
 
-
<td width="200">861<td>
 
-
<td width="200">pSB1A2<td>
 
-
<td width="200">pMB1<td>
 
-
<td width="200">2079<td>
 
-
<td width="200">2940<td>
 
-
<td width="200">Amp<td>
 
-
<td width="350">miniprepped<td>
 
-
<td width="50">ok<td>
 
-
<td width="50">[[Image:Chiba check.png]]<td>
 
-
</tr>
 
-
<tr>
 
-
<td width="200">[http://partsregistry.org/Part:BBa_K084005 BBa_K084005]</td>
 
-
<td width="300">ptet+rbs+LasR</td>
 
-
<td width="200">861<td>
 
-
<td width="200">pSB3K3<td>
 
-
<td width="200">p15A<td>
 
-
<td width="200">2750<td>
 
-
<td width="200">3611<td>
 
-
<td width="200">Kan<td>
 
-
<td width="350">miniprepped<td>
 
-
<td width="50">Not yet<td>
 
-
<td width="50">[[Image:Chiba check.png]]<td>
 
-
</tr>
 
-
<tr>
 
-
<td width="200">[http://partsregistry.org/Part:BBa_K084006 BBa_K084006]</td>
 
-
<td width="300">ptet+rbs+CinR</td>
 
-
<td width="200">867<td>
 
-
<td width="200">pSB1A2<td>
 
-
<td width="200">pMB1<td>
 
-
<td width="200">2079<td>
 
-
<td width="200">2946<td>
 
-
<td width="200">Amp<td>
 
-
<td width="350">miniprepped<td>
 
-
<td width="50">ok<td>
 
-
<td width="50">[[Image:Chiba check.png]]<td>
 
-
</tr>
 
-
<tr>
 
-
<td width="200">[http://partsregistry.org/Part:BBa_K084006 BBa_K084006]</td>
 
-
<td width="300">ptet+rbs+CinR</td>
 
-
<td width="200">867<td>
 
-
<td width="200">pSB3K3<td>
 
-
<td width="200">p15A<td>
 
-
<td width="200">2750<td>
 
-
<td width="200">3617<td>
 
-
<td width="200">Kan<td>
 
-
<td width="350">miniprepped<td>
 
-
<td width="50">Not yet<td>
 
-
<td width="50">[[Image:Chiba check.png]]<td>
 
-
</tr>
 
-
</table>
 
-
 
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===Reporters===
 
-
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000" center>
 
-
<tr>
 
-
<td width="200">Part Name</td>
 
-
<td width="200">Sub Parts</td>
 
-
<td width="200">Part Length(bp)<td>
 
-
<td width="200">Plasmid Vector<td>
 
-
<td width="200">Origin<td>
 
-
<td width="300">Part Length(bp)<td>
 
-
<td width="200">Part and vector length(bp)<td>
 
-
<td width="200">Resistance<td>
 
-
<td width="200">Status<td>
 
-
<td width="200">Sequenced<td>
 
-
<td width="200">Sent<td>
 
-
</tr>
 
-
<tr>
 
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<td width="200">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084002 BBa_K084002]</td>
 
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<td width="300">rbs+venusYFP(yeast optimized)</td>
 
-
<td width="200">767<td>
 
-
<td width="200">pSB1A2<td>
 
-
<td width="200">pMB1 <td>
 
-
<td width="200">2079<td>
 
-
<td width="200">3915<td>
 
-
<td width="200">Amp<td>
 
-
<td width="350">miniprepped<td>
 
-
<td width="50">no<td>
 
-
<td width="50">no<td>
 
-
</tr>
 
-
<tr>
 
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<td width="200">[http://partsregistry.org/Part:BBa_K084003 BBa_K084003]</td>
 
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<td width="300">ptet+LuxR+plux+venusYFP(yeast optimized)</td>
 
-
<td width="200">1836<td>
 
-
<td width="200">pSB1A2<td>
 
-
<td width="200">pMB1<td>
 
-
<td width="200">2079<td>
 
-
<td width="200">3915<td>
 
-
<td width="200">Amp<td>
 
-
<td width="350">miniprepped<td>
 
-
<td width="50">OK<td>
 
-
<td width="50">[[Image:Chiba check.png]]<td>
 
-
</tr>
 
-
</table>
 
-
==Parts Status(Parts used by Chiba)==
 
-
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000" center>
 
-
<tr>
 
-
<td width="200">Part Name</td>
 
-
<td width="200">Sub Parts</td>
 
-
<td width="200">Part Length(bp)<td>
 
-
<td width="200">Plasmid Vector<td>
 
-
<td width="200">Origin<td>
 
-
<td width="300">Part Length(bp)<td>
 
-
<td width="200">Part and vector length(bp)<td>
 
-
<td width="200">Resistance<td>
 
-
<td width="200">Status<td>
 
-
<td width="200">Sequenced<td>
 
-
</tr>
 
-
<tr>
 
-
<td width="200">[http://partsregistry.org/Part:BBa_T9002 BBa_T9002]</td>
 
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<td width="300">ptet+LuxR+plux+GFP</td>
 
-
<td width="200">1945<td>
 
-
<td width="200">pSB1A3<td>
 
-
<td width="200">pMB1<td>
 
-
<td width="200">2257<td>
 
-
<td width="200">4102<td>
 
-
<td width="200">Amp<td>
 
-
<td width="350">Glycerol stock in -80/miniprepped<td>
 
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<td width="50">OK<td>
 
-
</tr>
 
-
<tr>
 
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<td width="200">[http://partsregistry.org/Part:BBa_S03623 BBa_S03623]</td>
 
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<td width="300">ptet+LuxI</td>
 
-
<td width="200">723<td>
 
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<td width="200">pSB1AC3<td>
 
-
<td width="200">pMB1<td>
 
-
<td width="200">3005<td>
 
-
<td width="200">3778<td>
 
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<td width="200">Amp/Cm<td>
 
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<td width="350">Glycerol stock in -80/miniprepped<td>
 
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<td width="50">OK<td>
 
-
</tr>
 
-
</table>
 
-
 
-
 
-
{| style="color:white;" cellpadding="3" cellspacing="3" border="0" width="100%" align="center" class="menu" |
 
-
!align="center"|[[Team:Chiba|Home]]
 
-
!align="center"|[[Team:Chiba/Team|The Team]]
 
-
!align="center"|[[Team:Chiba/Project|The Project]]
 
-
!align="center"|[[Team:Chiba/Parts|Parts Submitted to the Registry]]
 
-
!align="center"|[[Team:Chiba/Reference|Reference]]
 
-
!align="center"|[[Team:Chiba/Notebook|Notebook]]
 
-
!align="center"|[[Team:Chiba/Acknowledgements|Acknowledgements]]
 
-
|}
 

Revision as of 18:40, 26 October 2010

Contents

Parts Status(Parts created by Chiba)

Hybrid promoter

Reporters

Part Name Sub Parts Part Length(bp) Plasmid Vector Origin Part Length(bp) Part and vector length(bp) Resistance Status Sequenced Sent
BBa_K396002 pLux/cI434 Hybrid promoter 61 pSB1C3 pMB1 2072 2133 Cm miniprepped ok no

Generator

Part Name Sub Parts Part Length(bp) Plasmid Vector Origin Part Length(bp) Part and vector length(bp) Resistance Status Sequenced Sent
BBa_K396003 UV light GFP with LVA tag 723 pSB1C3 pMB1 2072 2795 Cm miniprepped ok no

Parts Status(Parts used by Chiba)

Part Name Sub Parts Part Length(bp) Plasmid Vector Origin Part Length(bp) Part and vector length(bp) Resistance Status Sequenced Sent
BBa_K396001 pLux/cI434 hybrid promoter+RBS weak+GFPuv LVA tag 806 pSB1C3 pMB1 2072 2878 Cm miniprepped ok ok
BBa_K396011 Prom-luxR 814 pSB1C3 pMB1 2072 2886 Cm miniprepped ok ok
Part Name Sub Parts Part Length(bp) Plasmid Vector Origin Part Length(bp) Part and vector length(bp) Resistance Status Sequenced
BBa_R0061 Promoter (HSL-mediated luxR repressor) 30 pSB2K3 F and P1 lytic 4425 4455 Kan miniprepped OK
BBa_J01101 ptet+cI 992 pSB1A2 pMB1 2079 3071 Amp Glycerol stock in -80/miniprepped OK